Abstract:
:Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species from other geographical areas using gene sequences encoding cytochrome b (cytb) and internal transcribed spacer 2 (its2). Based on cytochrome b sequence analysis, L. tropica strains from Pakistan and other geographical regions were differentiated into two genotype groups, A and B. Within the province, five distinct L. tropica genotypes were recognized; two in group A, three in group B. Two L. infantum isolates from the province were closely associated with both Afro-Eurasian and American species of the Leishmania donovani complex, including Leishmania chagasi, L. infantum and L. donovani from Sudan and Ethiopia; while a third L. infantum isolate could not be differentiated from visceralizing Kenyan and Indian L. donovani. We observed apposite phylogenetic placement of CL-causing L. tropica and L. infantum from Khyber Pakhtunkhwa. Affinities ascribed to Leishmania spp. From the region are valuable in tracing potential importation of leishmaniasis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Khan NH,Messenger LA,Wahid S,Sutherland CJdoi
10.1016/j.exppara.2016.05.006subject
Has Abstractpub_date
2016-08-01 00:00:00pages
61-6eissn
0014-4894issn
1090-2449pii
S0014-4894(16)30096-0journal_volume
167pub_type
杂志文章abstract::Antimony unresponsiveness in mucocutaneous and visceral leishmaniasis is a serious clinical problem. Information on the mechanisms and characteristics of drug resistance in parasites that suggest chemotherapeutic strategies to overcome resistance is of practical importance. We developed nine lines of Leishmania resist...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90173-2
更新日期:1989-07-01 00:00:00
abstract::The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.03.012
更新日期:2008-07-01 00:00:00
abstract::The biochemical properties of the enzyme responsible for nematode "activated L-serine sulphydrase" activity (L-cysteine + R-SH----cysteine thioether + H2S) have been investigated using primarily the gastro-intestinal nematodes Nippostrongylus brasiliensis and Haemonchus contortus. The activated L-serine sulphydrase en...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90048-f
更新日期:1992-03-01 00:00:00
abstract::In Trypanosoma brucei the GPI-anchored variant surface glycoprotein (VSG) represents approximately 90% of cell surface protein and a major proportion of endoplasmic reticulum (ER) biosynthetic output. We identified four trypanosomatid-specific genes encoding candidate ER-resident proteins; all were required for normal...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.01.015
更新日期:2010-07-01 00:00:00
abstract::The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(02)00113-3
更新日期:2002-06-01 00:00:00
abstract::The search for novel therapeutic candidates against animal trypanosomiasis is an ongoing scientific endevour because of the negative impacts of the disease to the African livestock industry. In this study, the in vivo therapeutic potentials of phytol toward Trypanosoma congolense infection and the inhibitory effects o...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2020.107943
更新日期:2020-09-01 00:00:00
abstract::In March 2010, a 35 year-old HIV/AIDS female patient was admitted to hospital to start treatment with Highly Active Antiretroviral Therapy (HAART) since during a routine control a dramatic decrease in the CD4(+) levels was detected. At this stage, a nasal swab from each nostril was collected from the patient to includ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.02.014
更新日期:2014-11-01 00:00:00
abstract::We have studied inflammatory reactions in the mesenteric tissue of mice infected with Schistosoma mansoni. Perivascular tissue contained diffuse infiltrates of macrophages, eosinophilic granulocytes and lymphocytes. Angiogenesis in the perivascular adipose tissue was associated with superficial plasmacytogenic foci. P...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90203-m
更新日期:1992-06-01 00:00:00
abstract::Acquisition of iron from lactoferrin and transferrin by a parasitic protozoon Tritrichomonas foetus has been studied in vitro. Specific, time-dependent, and saturable binding of iodinated ligands to the outer membrane of T. foetus at 4 degrees C was demonstrated for 125I-labeled lactoferrin only. About 1.7 x 10(5) bin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0068
更新日期:1996-07-01 00:00:00
abstract::Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecifi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90130-x
更新日期:1986-02-01 00:00:00
abstract::The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5' domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90133-h
更新日期:1991-02-01 00:00:00
abstract::Trypanosoma rangeli is a parasite of a numerous wild and domestic animals, presenting wide geographical distribution and high immunological cross-reactivity with Trypanosoma cruzi, which may lead to misdiagnosis. T. rangeli has a complex life cycle, involving distinct morphological and functional forms in the vector. ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.11.015
更新日期:2006-04-01 00:00:00
abstract::In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival r...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1997.4148
更新日期:1997-03-01 00:00:00
abstract::The chemical properties of the primary antileishmanial agent sodium stibogluconate (Pentostam), and the interaction of Pentostam with Leishmania mexicana amastigotes, have been investigated with the aid of [125Sb]Pentostam. The molecular weight by P2 chromatography showed [125Sb]Pentostam to be of multiple species of ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(88)90012-4
更新日期:1988-10-01 00:00:00
abstract::Trypanosoma cruzi infection in humans and experimental animals often results in a chronic heart and gut inflammation and a dysfunction known as Chagas' disease. Previous studies have shown that the cellular infiltrate in the hearts of animals with chronic Chagas' disease consists mainly of CD8+ T cells. In this study,...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0106
更新日期:1996-11-01 00:00:00
abstract::Hyperinfective strongyloidiasis involving the threadworm , Strongyloides stercoralis, is well known in humans and primates. Although this nematode also frequently parasitizes dogs, canine hyperinfective strongyloidiasis has not been reported. To determine whether a fulminant pattern of nematode development can occur i...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(84)90103-6
更新日期:1984-06-01 00:00:00
abstract::In this work CD4-knockout mice were used as a model to analyse the role of CD4+ T cells in the antibody response against Echinococcus granulosus immunization or experimental infection. Results obtained with mice immunized with protoscolex antigens indicated that these contain T-independent antigens. After infection, C...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.04.024
更新日期:2008-08-01 00:00:00
abstract::In 1912 Blastocystis hominis was identified as a new species and classified as a yeast (Brumpt 1912). In the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus Schizosaccharomyces (discussed by Zierdt et al. 1967). Apart from an occasional case report, the classifica...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90110-0
更新日期:1989-04-01 00:00:00
abstract::Shigellosis is a serious public health problem in Korea, because large outbreaks of Shigella sonnei infections were recorded in many parts of the country during the period 1998-2000. However, the epidemiological features of shigellosis are not well known. In this study, we devised conditions suitable for the growth an...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.08.002
更新日期:2007-02-01 00:00:00
abstract::The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1110
更新日期:1994-12-01 00:00:00
abstract::By using a sensitive fluorometric method with Val-Leu-Gly-Arg-3-amino-9-ethylcarbazole (VLGR-AEC) as a substrate, two endopeptidase activities were identified in two fractions of Sephacryl S-200 gel filtration from soluble P. berghei and P. chabaudi extracts. Controls with normal mouse erythrocytes, with leukocytes, a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(87)90013-0
更新日期:1987-08-01 00:00:00
abstract::The effect of experimental exposure of Bradybaena similaris by Eurytrema coelomaticum on the cholesterol and triglycerides total levels circulating in the hemolymph and the neutral lipids in the digestive gland-gonad (DGG) complex of the host snail were studied. In this study, snails were dissected after one, two, and...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.06.010
更新日期:2014-09-01 00:00:00
abstract::The differential effect of fox and pig bile and its corresponding low molecular weight fraction (LMW) was investigated on the in vitro invasion of MDCK-AA7 epithelial cell monolayers by Trichinella spiralis muscle larvae. Seven invasion experiments were performed and a total of 274 cell monolayers were examined. Fox a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.05.013
更新日期:2010-12-01 00:00:00
abstract::Leishmaniasis is one of the most neglected tropical infectious diseases in the world. The emergence of drug resistance and toxicity and the high cost of the available drugs with a lack of new anti-leishmanial drugs highlight the need to search for newer therapies with anti-leishmanial activities. Due to the mesenchyma...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2020.108063
更新日期:2021-01-04 00:00:00
abstract::Trichomonas vaginalis is a human urogenital pathogen that causes trichomoniasis, the most common nonviral, parasitic sexually transmitted infection in the world. At present, little is known regarding the degree of strain variability of T. vaginalis. A classification method for T. vaginalis strains would be a useful to...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.10.011
更新日期:2015-12-01 00:00:00
abstract::The ring stages of Plasmodium falciparum within red blood cells cultured with complete medium stop growing when transferred to a basic medium containing RPMI plus fatty acid-free bovine serum albumin and dialyzable factors from human serum. Growth and multiplication can be partially restored by the addition of lipopro...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(83)90008-5
更新日期:1983-02-01 00:00:00
abstract::Spleen cells from BALB/c mice were fused with myeloma cells following infection of the mice with Trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. A monoclonal antibody (Mab), designated as TS 3G6 which did not react with sera or tissue extracts from noninfected mice, rats, and gu...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90018-r
更新日期:1991-08-01 00:00:00
abstract::Given that signal sequences between secreted proteins of different species can be interchanged, it is reasonable to expect that both mammalian and yeast signal sequence trapping (SST) systems would secrete Haemonchus contortus proteins with similar efficiency and quality. To determine if H. contortus cDNAs that contai...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.12.017
更新日期:2008-05-01 00:00:00
abstract::The role of oxidative stress in the pathogenicity of acanthamoebiasis is an important aspect of the intricate and complex host-parasite relationship. The aim of this experimental study was to determine oxidative stress through the assessment of lipid peroxidation product (LPO) levels and antioxidant defense mechanism ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.09.002
更新日期:2018-10-01 00:00:00
abstract::Immunity to T. cruzi is complex, involving among other components, antibody production, CD4+ helper cells, CD8+ T cells as both regulators and effectors of immunity, and possibly, double-negative T cells. In addition, several of these components have been implicated in pathogenesis in the chronic infection. Although t...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/0014-4894(91)90013-m
更新日期:1991-07-01 00:00:00