Abstract：
:Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inflammation and complement activation, some of which are considered the host's first line of defense to hematophagous and/or blood dueling parasites. Thus, it is hypothesized that ticks use serpins to evade host defense, facilitating parasitism. This study describes eighteen full-length cDNA sequences encoding serpins identified in Rhipicephalus (Boophilus) microplus, here named RmS 1-18 (R. microplus serpin). Spatial and temporal transcriptional profiling demonstrated that R. microplus serpins are transcribed during feeding, suggesting their participation in tick physiology regulation. We speculate that the majority of R. microplus serpins are conserved in other ticks, as indicated by phylogeny analysis. Over half of the 18 RmSs are putatively functional in the extracellular environment, as indicated by putative signal peptides on 11 of 18 serpins. Comparative modeling and structural-based alignment revealed that R. microplus serpins in this study retain the consensus secondary of typical serpins. This descriptive study enlarges the knowledge on the molecular biology of R. microplus, an important tick species.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Tirloni L,Seixas A,Mulenga A,Vaz Ida S Jr,Termignoni Cdoi
10.1016/j.exppara.2013.12.001subject
Has Abstractpub_date
2014-02-01 00:00:00pages
25-34eissn
0014-4894issn
1090-2449pii
S0014-4894(13)00304-4journal_volume
137pub_type
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pub_type： 杂志文章,评审
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4318
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journal_title：Experimental parasitology
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pub_type： 杂志文章
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更新日期：1986-02-01 00:00:00
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journal_title：Experimental parasitology
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