Abstract:
:In the present study, the gene expression of three multidrug resistance (MDR) and resistance-associated protein (MRP) transport proteins or efflux pumps was characterized and the phenotypic evidence for such pumps was demonstrated in cultured Madin-Darby canine kidney (MDCK) cells. A gradient for the fluorescent probe calcein was established between parasite and host cell suggestive of a parasite extrusion pump at the parasite-host interface. This gradient was decreased in a glucose-free medium containing 2-deoxyglucose or 3-O-methylglucose, by probenecid, and by the isoflavonoid, narigenin, suggesting that the calcein extrusion was energy-dependent and involved an MRP-like pump. While neither MDR or MRP inhibiters significantly affected transcript levels of any of the ABC transporters, transcript levels of the Cryptosporidium parvum ABC protein (CpABC1), an MRP transporter, were consistently expressed 4 logs higher than either CpABC3 or CpABC2, suggesting a prominent role in the intracellular stages of the parasite.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Bonafonte MT,Romagnoli PA,McNair N,Shaw AP,Scanlon M,Leitch GJ,Mead JRdoi
10.1016/j.exppara.2004.03.012keywords:
subject
Has Abstractpub_date
2004-03-01 00:00:00pages
126-34issue
3-4eissn
0014-4894issn
1090-2449pii
S001448940400058Xjournal_volume
106pub_type
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