Abstract:
:Advanced molecular biology techniques are currently used to develop new effective strategies against fasciolosis. Assessment of the quality of extracted total RNA is an important step prior to commencing many molecular biology methods such as transcriptomics. However, RNA quality assessment is complicated for some organisms, including Fasciola hepatica, by the absence of a 28S rRNA peak/band, when assessed with modern protocols. In this study, electrophoretic profiles of F. hepatica ribosomal RNAs were evaluated using microfluidics capillary based and conventional non-denaturing gel electrophoresis methods. An important modification to recommended protocols, the exclusion of heat-denaturation step, in the microfluidics capillary based electrophoresis is critical to visualise the expected 28S rRNA and obtain an RNA integrity number (RIN). The intensity of the 28S rRNA band is reduced by the effect of non-denaturing gel electrophoresis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Haçarız O,Sayers Gdoi
10.1016/j.exppara.2013.07.026subject
Has Abstractpub_date
2013-10-01 00:00:00pages
426-9issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(13)00212-9journal_volume
135pub_type
杂志文章abstract::The sphingolipids biosynthesis pathway generates bioactive molecules crucial to the regulation of physiological processes. We have recently reported that DAG (diacylglycerol) generated during sphingomyelin synthesis, plays an important role in PKC (protein kinase C) activation, necessary for the transit through the ce...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.02.011
更新日期:2009-06-01 00:00:00
abstract::In an attempt to identify the tissue sources of biochemically purified antigenic fractions of Fasciola hepatica and Schistosoma mansoni, antisera were tested against plastic-embedded sections of worms of various ages by an indirect fluorescent-antibody-labeling technique. Antibodies prepared against antigens purified ...
journal_title:Experimental parasitology
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abstract::One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...
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journal_title:Experimental parasitology
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doi:10.1016/j.exppara.2008.10.008
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abstract::Two species of macaques, including two Macaca fascicularis from the Philippines, two M. fascicularis from Mauritius, and one Macaca mulatta, were experimentally infected with blood stages of Plasmodium coatneyi and followed during their clinical, parasitological, biological, and immunological evolution. Plasma cytokin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1999.4342
更新日期:1999-01-01 00:00:00
abstract::Acanthamoeba spp., known to cause keratitis and granulomatous encephalitis in humans, are frequently isolated from a variety of water sources. Here we report for the first time the characterization of an Acanthamoeba sp. (ACC01) isolated from tap water in Brazil. This organism is currently being maintained in an axeni...
journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.12.001
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abstract::A drug-resistant Trypanosoma congolense strain with predetermined curative doses (CD50 and CD90) of samorin at 13.9 +/- 1.02 and 20.3 +/- 1.13 mg/kg body weight, respectively, was cyclically transmitted through tsetse flies and by syringe passages in mice in the absence of drug pressure. The changing levels of drug se...
journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.06.005
更新日期:2014-09-01 00:00:00
abstract::Cutaneous Leishmaniasis (CL) caused by Leishmania aethiopica is a public health and social problem with a sequel of severe and mutilating skin lesions. It is manifested in three forms: localized CL (LCL), mucosal CL (MCL) and diffuse CL (DCL). Unresponsiveness to sodium stibogluconate (Sb(V)) is common in Ethiopian CL...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.01.022
更新日期:2013-05-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.02.018
更新日期:2007-08-01 00:00:00
abstract::Traditional classification of Demodex mites by hosts and phenotypic characteristics is defective because of environmental influences. In this study, we proposed molecular identification of four phenotypes of two human Demodex species based on mitochondrial cox1 fragments for the first time. Mites collected from suffer...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.04.003
更新日期:2014-07-01 00:00:00
abstract::Giardia lamblia is a protozoan pathogen with distinct cytoskeletal structures, including median bodies and eight flagella. In this study, we examined components comprising G. lamblia flagella. Crude flagellar extracts were prepared from G. lamblia trophozoites, and analyzed by two-dimensional (2-D) gel electrophoresis...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.07.010
更新日期:2013-10-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.02.024
更新日期:2005-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.4115
更新日期:1997-01-01 00:00:00
abstract::The extracellular metalloproteinases of the insect trypanosomatid Crithidia guilhermei were characterized through the incorporation of different protein substrates (gelatin, casein, haemoglobin, and bovine serum albumin) into SDS-PAGE. Two gelatinases (60 and 80 kDa) showed ability to degrade casein as well and a 67-k...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1070
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.02.015
更新日期:2005-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(85)90024-4
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abstract::Resistance and sensitivity to normal human serum (NHS) of Trypanosoma congolense, a parasite believed to cause disease in animals only, were investigated in vivo as well as in vitro. Our results indicate that like Trypanosoma brucei, T. congolense can be grouped into three different phenotypes according to its resista...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2020.107919
更新日期:2020-08-01 00:00:00
abstract::In murine Schistosoma mansoni infections, fewer adult worms develop in male than in female mice infected with the same number of cercariae. To evaluate a potential role for testosterone in this phenomenon, testosterone levels were manipulated in groups of CBA/J mice that were then infected and monitored for survival r...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.12.002
更新日期:2012-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(90)90109-p
更新日期:1990-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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