Susceptibility of clinical isolates of Leishmania aethiopica to miltefosine, paromomycin, amphotericin B and sodium stibogluconate using amastigote-macrophage in vitro model.

abstract：:In the search of new alternatives for neurocysticercosis treatment, the cysticidal activity of organic extracts of Teloxys graveolens was evaluated. The in vitro activity of hexane, ethyl acetate and methanol extracts against Taenia crassiceps cysts was tested and the selectivity index relative to human fibroblasts wa...

journal_title：Experimental parasitology

authors： Palomares-Alonso F,Rojas-Tomé IS,Juárez Rocha V,Palencia Hernández G,González-Maciel A,Ramos-Morales A,Santiago-Reyes R,González-Hernández IE,Jung-Cook H

更新日期：2015-09-01 00:00:00

abstract：:Nitric oxide (NO) has diverse biological functions. Numerous studies have documented NO's biosynthetic pathway in a wide variety of organisms. Little is known, however, about NO production in intraerythrocytic Plasmodium falciparum. Using diaminorhodamine-4-methyl acetoxymethylester (DAR-4M AM), a fluorescent indicato...

journal_title：Experimental parasitology

authors： Ostera G,Tokumasu F,Oliveira F,Sa J,Furuya T,Teixeira C,Dvorak J

更新日期：2008-09-01 00:00:00

abstract：:A method is described for the isolation of peroxisomes from mitochondria-enriched fractions obtained from both species of nematodes. The distributions of these organelles are characterized after density gradient centrifugation in sucrose or Percoll by urate oxidase and catalase activities. The possession of peroxisome...

journal_title：Experimental parasitology

authors： Paget TA,Fry M,Lloyd D

更新日期：1990-08-01 00:00:00

abstract：:Freeze-fracturing has been used to study the architecture of the pellicular complex of the gametocytes of Plasmodium falciparum. The gametocyte is surrounded by three membranes and a layer of subpellicular microtubules. During freeze-fracturing, each of the three membranes is split along its hydrophobic interior to yi...

journal_title：Experimental parasitology

authors： Meszoely CA,Erbe EF,Steere RL,Trosper J,Beaudoin RL

更新日期：1987-12-01 00:00:00

abstract：:A cell-free culture system for Cryptosporidium parvum was analysed using scanning electron microscopy (SEM) to characterise life cycle stages and compare gene expression in cell-free culture and cell culture using HCT-8 cells. Cryptosporidium parvum samples were harvested at 2 h, 8 h, 14 h, 26 h, 50 h, 74 h, 98 h, 122...

journal_title：Experimental parasitology

authors： Yang R,Elankumaran Y,Hijjawi N,Ryan U

更新日期：2015-06-01 00:00:00

abstract：:The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F. hepatica, rats were treated with a single 100mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after ...

journal_title：Experimental parasitology

authors： Keiser J,Morson G

更新日期：2008-05-01 00:00:00

abstract：:Though parasite cyclophilins are promising new drug targets, Eimeria tenella cyclophilins have not been characterized yet. Here, we describe an 89kDa cyclophilin, designated EtCYP89. It is expressed throughout the developmental cycle of E. tenella, both in the intracellular stages in chicken and in extracellular sporu...

journal_title：Experimental parasitology

authors： Hosse RJ,Krücken J,Bierbaum S,Greif G,Wunderlich F

更新日期：2008-02-01 00:00:00

abstract：:Giardia lamblia, an early diverging eukaryote that infects several species including humans and a major agent of water-borne diarrhea throughout the world, can be infected with a double-stranded RNA virus, giardiavirus (GLV). A chimeric GLV cDNA and green fluorescent protein (GFP) according to the cis-acting signals o...

journal_title：Experimental parasitology

authors： Liu Q,Zhang X,Li J,Ying J,Chen L,Zhao Y,Wei F,Wu T

更新日期：2005-03-01 00:00:00

abstract：:The yellow fever mosquito, Aedes aegypti, expresses a gene, Apyrase (Apy), that encodes an ATP-diphosphohydrolase. The product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. Apy gene expression is limited to the cells of the distal-lateral and medial lobe...

journal_title：Experimental parasitology

authors： Smartt CT,Kim AP,Grossman GL,James AA

更新日期：1995-11-01 00:00:00

abstract：:We report here the pre-mRNA 3' end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3' end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identifie...

journal_title：Experimental parasitology

authors： López-Camarillo C,Orozco E,Marchat LA

更新日期：2005-07-01 00:00:00

abstract：:The sequential changes in the histology in scabietic lesions were investigated for hosts (rabbits) during sensitization and reinfestation with Sarcoptes scabiei. Experimentally infested naive hosts developed heavy scabies infestations but 65% of the hosts showed induced immunity (resistance) to reinfestation. All host...

journal_title：Experimental parasitology

authors： Arlian LG,Rapp CM,Vyszenski-Moher DL,Morgan MS

更新日期：1994-02-01 00:00:00

abstract：:The compound 2-hydroxy-3-(1'-propen-3-phenyl)-1,4-naphthoquinone (PHNQ6) was evaluated for activity against Toxoplasma gondii, alone or combined with sulfadiazine. Treatment with PHNQ6 combined with sulfadiazine protected at least 70 and 90% of mice infected with RH and EGS strains, respectively. Mice were treated wit...

journal_title：Experimental parasitology

authors： Ferreira RA,Oliveira AB,Ribeiro MF,Tafuri WL,Vitor RW

更新日期：2006-06-01 00:00:00

abstract：:A molecular study was carried out to incriminate sand fly vectors of cutaneous leishmaniasis (CL) in rural areas of Sarakhs district, Khorassane-Razavi Province, northeastern Iran, in 2011. Sand flies of Sergentomyia with three species and Phlebotomus with six species respectively comprised 73.3% and 26.7% of the spec...

journal_title：Experimental parasitology

authors： Bakhshi H,Oshaghi MA,Abai MR,Rassi Y,Akhavan AA,Sheikh Z,Mohtarami F,Saidi Z,Mirzajani H,Anjomruz M

更新日期：2013-10-01 00:00:00

abstract：:Transforming growth factor-β (TGF-β) signaling pathway is documented to participate in liver fibrosis via multifactorial mechanisms. microRNA Let-7b (Let-7b) has been proved to alleviate cell fibrosis through regulating TGF-β receptor I (TβRI), but whether it is involved in Schistosomiasis liver fibrosis (SLF) has not...

journal_title：Experimental parasitology

authors： Tang N,Wu Y,Cao W,Liang Y,Gao Y,Hu L,Yang Q,Zhou Y,Tang F,Xiao J

更新日期：2017-11-01 00:00:00

abstract：:The infection with blood stages of Plasmodium chabaudi chabaudi (AS) was followed in BALB/c and DBA/2 mice. Both strains show a peak parasitemia by 7-9 days after infection, display splenic hypercellularity of T and B cells, thymic atrophy, nearly complete depletion of B cells in the bone marrow, and mount comparable ...

journal_title：Experimental parasitology

authors： Seixas E,Ostler D

更新日期：2005-08-01 00:00:00

abstract：:Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria par...

journal_title：Experimental parasitology

authors： Hillman AE,Yang R,Lymbery AJ,Thompson RCA

更新日期：2016-11-01 00:00:00

abstract：:A full-length cDNA encoding the 24-kDa flagellar Ca(2+)-binding protein (FCaBP) of the Dm28c clone of Trypanosoma cruzi was cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of other T. cruzi strains revealed greater than 97% sequence conservation. FCaBP-like genes are fo...

journal_title：Experimental parasitology

authors： Maldonado RA,Linss J,Thomaz N,Olson CL,Engman DM,Goldenberg S

更新日期：1997-07-01 00:00:00

abstract：:Cytoadherence of Plasmodium falciparum-infected erythrocytes to amelanotic melanoma cells was pH dependent; increased adherence was observed in the pH range of 6.1 to 6.8 and was greatest between pH 6.6 and 6.8 Ca2+ promoted cytoadherence, but at higher concentrations (40-50 mM) than is usually the case for cell-cell ...

journal_title：Experimental parasitology

authors： Crandall I,Smith H,Sherman IW

更新日期：1991-10-01 00:00:00

abstract：:A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtaine...

journal_title：Experimental parasitology

authors： Kumagai T,Maruyama H,Hato M,Ohmae H,Osada Y,Kanazawa T,Ohta N

更新日期：2005-01-01 00:00:00

abstract：:Previous results from our laboratory using pharmacological approaches suggested a role for nitric oxide (NO) in the host defense against the human filarial parasite, Brugia malayi. We sought to determine whether a complementary genetic approach, using mice homozygous for a targeted mutation in the gene encoding induci...

journal_title：Experimental parasitology

authors： Ganley L,Babu S,Rajan TV

更新日期：2001-05-01 00:00:00

abstract：:Trypanosoma cruzi is a rare example of an eukaryote that has genes for two threonine proteases: HslVU complex and 20S proteasome. HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase. In this study, we expressed and obtained specific antibodies to HslU and ...

journal_title：Experimental parasitology

authors： Barboza NR,Cardoso J,de Paula Lima CV,Soares MJ,Gradia DF,Hangai NS,Bahia MT,de Lana M,Krieger MA,Guerra de Sá R

更新日期：2012-02-01 00:00:00

abstract：:The aim of this study was to develop a simple and reliable method to determine the viability of Giardia intestinalis after incubation with an anti-giardial agent by using a colorimetric method. Factors that may affect the optical density value were systematically evaluated. The most suitable conditions were obtained w...

journal_title：Experimental parasitology

authors： Hounkong K,Sawangjaroen N,Phongpaichit S

更新日期：2011-02-01 00:00:00

abstract：:Using nucleotide variation in the first internal transcribed spacer of nuclear ribosomal DNA, five different genotypes (designated G1-G5) have been identified and the preponderance of genotype G1 in humans and of genotype G3 in pigs led to the proposal that parasites bearing the two genotypes have an affinity for a pa...

journal_title：Experimental parasitology

authors： Peng W,Yuan K,Peng G,Qiu L,Dai Z,Yuan F,Hu Y,Hu N

更新日期：2012-05-01 00:00:00

abstract：:Cryptosporidiosis, caused by the protozoan parasite Cryptosporidium, represents the major public health concern of water utilities in developed nations due to its small size, resistance to disinfection and ability to be shed in large numbers in faeces. In Australia, recreational access is not allowed on direct supply ...

journal_title：Experimental parasitology

authors： Loganthan S,Yang R,Bath A,Gordon C,Ryan U

更新日期：2012-08-01 00:00:00

abstract：:Cercariae of Schistosoma mansoni produce a wide variety of eicosanoids when stimulated by 3.3 mM linoleate. High-performance liquid chromatography indicated that 10(-5) M esculetin dramatically decreased eicosanoid production by cercariae. Ibuprofen (10(-4) M) also decreased eicosanoid production, but to a lesser exte...

journal_title：Experimental parasitology

authors： Salafsky B,Fusco AC

更新日期：1985-08-01 00:00:00

abstract：:Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from various part of the world, also in Poland, with increasing frequency, particularl...

journal_title：Experimental parasitology

authors： Chomicz L,Padzik M,Graczyk Z,Starosciak B,Graczyk TK,Naprawska A,Oledzka G,Szostakowska B

更新日期：2010-09-01 00:00:00

abstract：:This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...

journal_title：Experimental parasitology

authors： Ribeiro MF,Bastos CV,Vasconcelos MM,Passos LM

更新日期：2009-07-01 00:00:00

abstract：:Allelic variation in the Plasmodium falciparum merozoite surface protein 1 (MSP1) gene is expressed as an association of allelic types in variable blocks. In this study, a PCR strategy that can detect 24 different MSP1 association types was used to investigate allelic variation in the MSP1 gene. We identified 236 dist...

journal_title：Experimental parasitology

authors： Kaneko O,Kimura M,Kawamoto F,Ferreira MU,Tanabe K

更新日期：1997-05-01 00:00:00

abstract：:Resistance and sensitivity to normal human serum (NHS) of Trypanosoma congolense, a parasite believed to cause disease in animals only, were investigated in vivo as well as in vitro. Our results indicate that like Trypanosoma brucei, T. congolense can be grouped into three different phenotypes according to its resista...

journal_title：Experimental parasitology

authors： Xong HV,De Baetselier P,Pays E,Magez S

更新日期：2002-10-01 00:00:00

abstract：:There are alterations in the proteins synthesized during different stages of development of Schistosoma mansoni. The protein profiles at different stages were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When stained by Coomassie blue, no significant differences were seen in protein profiles ...

journal_title：Experimental parasitology

authors： Yuckenberg PD,Poupin F,Mansour TE

更新日期：1987-06-01 00:00:00