Giardia lamblia: stable expression of green fluorescent protein mediated by giardiavirus.

Abstract:

:Giardia lamblia, an early diverging eukaryote that infects several species including humans and a major agent of water-borne diarrhea throughout the world, can be infected with a double-stranded RNA virus, giardiavirus (GLV). A chimeric GLV cDNA and green fluorescent protein (GFP) according to the cis-acting signals of the GLV genome required for expression of foreign gene was constructed and its in vitro transcript was electroporated into GLV-infected G. lamblia trophozoites, GFP was expressed transiently. pGDH5/NEO/GLV was constructed by combining the neomycin resistance cassette in which the neomycin phosphotransferase gene was flanked by Giardia glutamate dehydrogenase (GDH) uncoding regions and the transcription cassette in which the chimera of GLV cDNA and GFP was located downstream from GDH gene promoter on a single plasmid. This plasmid was electroporated into G. lamblia and the transfectants persistently expressed GFP under G418 selection. This stable transfection system should provide a valuable tool for genetic study of G. lamblia.

journal_name

Exp Parasitol

authors

Liu Q,Zhang X,Li J,Ying J,Chen L,Zhao Y,Wei F,Wu T

doi

10.1016/j.exppara.2004.12.006

keywords:

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

181-7

issue

3

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(04)00204-8

journal_volume

109

pub_type

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