Abstract:
:Though parasite cyclophilins are promising new drug targets, Eimeria tenella cyclophilins have not been characterized yet. Here, we describe an 89kDa cyclophilin, designated EtCYP89. It is expressed throughout the developmental cycle of E. tenella, both in the intracellular stages in chicken and in extracellular sporulated oocysts and sporozoites. The EtCYP89 protein contains two Ser-rich domains in its NH2-terminus separated by a His-rich stretch. WD40 repeats are localized in the central part of the protein followed by a cyclophilin domain at the COOH-terminus. Both protein and genomic organization of EtCyp89 are conserved in comparison with its ortholog TgCyp81.6 in Toxoplasma gondii, except for the absence of a Ser- and His-rich NH2-terminus in TgCYP81.6. In particular, those 13 residues are conserved which are responsible for binding the anti-coccidial drug cyclosporine A.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Hosse RJ,Krücken J,Bierbaum S,Greif G,Wunderlich Fdoi
10.1016/j.exppara.2007.07.013subject
Has Abstractpub_date
2008-02-01 00:00:00pages
275-9issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(07)00204-4journal_volume
118pub_type
杂志文章abstract::A cell-free culture system for Cryptosporidium parvum was analysed using scanning electron microscopy (SEM) to characterise life cycle stages and compare gene expression in cell-free culture and cell culture using HCT-8 cells. Cryptosporidium parvum samples were harvested at 2 h, 8 h, 14 h, 26 h, 50 h, 74 h, 98 h, 122...
journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0068
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.4132
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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doi:10.1006/expr.1996.0036
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doi:10.1016/j.exppara.2009.01.001
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abstract::Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) a...
journal_title:Experimental parasitology
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更新日期:2009-12-01 00:00:00
abstract::Many methods have been proposed for removing contaminating host cells from mouse peritoneal exudates infected with Toxoplasma gondii tachyzoites. Of these, eight established methods were compared. They were density gradients, sonication and trypsin digestion, differential centrifugation, haemolysin digestion, filtrati...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(84)90080-8
更新日期:1984-04-01 00:00:00
abstract::Shigellosis is a serious public health problem in Korea, because large outbreaks of Shigella sonnei infections were recorded in many parts of the country during the period 1998-2000. However, the epidemiological features of shigellosis are not well known. In this study, we devised conditions suitable for the growth an...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.08.002
更新日期:2007-02-01 00:00:00
abstract::Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote grow...
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doi:10.1016/j.exppara.2009.11.007
更新日期:2010-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.08.003
更新日期:2008-02-01 00:00:00
abstract::A mode of action of the inducible treatment with trypsin for the development of Mesocestoides lineatus tetrathyridium to adult was analyzed by administering various agents effective on Ca2+-dependent metabolic pathways in the cells: protein kinase C activators such as a synthetic diacylglycerol, 1-oleoyl-2-acetylglyce...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90036-6
更新日期:1986-12-01 00:00:00
abstract::Investigation of protease activities during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigoes (metacyclo-genesis) revealed three major components with apparent molecular weights of 65, 52, and 40 kDa. The 65-kDa protease is a metacyclic trypomastigote stage-specific protease with an ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90006-i
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abstract::We performed a calibration of flotation in tube, McMaster and FLOTAC to determine the optimal flotation solution (FS) and the influence of faecal preservation for the diagnosis of Ancylostoma caninum in dogs, and compared the accuracy of the three copromicroscopic techniques. Among nine different FS, sodium chloride a...
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abstract::Two experiments were performed to determine whether oral administration of copper oxide capsules controlled helminthic infections in Lacaune sheep without acute collateral effects on animal health. In experiment 1, 48 multiparous lactating sheep (60.1 ± 8.5 kg) were stratified according to initial number of eggs (Haem...
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
abstract::The compound 2-hydroxy-3-(1'-propen-3-phenyl)-1,4-naphthoquinone (PHNQ6) was evaluated for activity against Toxoplasma gondii, alone or combined with sulfadiazine. Treatment with PHNQ6 combined with sulfadiazine protected at least 70 and 90% of mice infected with RH and EGS strains, respectively. Mice were treated wit...
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pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.12.024
更新日期:2010-06-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.04.010
更新日期:2006-12-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1997.4148
更新日期:1997-03-01 00:00:00
abstract::Amoebae from the genus Acanthamoeba are facultative pathogens of humans and other animals. In humans they most frequently infect the eye causing a sight threatening infection known as Acanthamoeba keratitis (AK), and also cause an often fatal encephalitis (GAE). A mannose-binding protein (MBP) has been identified as b...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.07.009
更新日期:2018-09-01 00:00:00
abstract::An efficient technique was developed for separating early and late stages of embryonic development in eggs of Heterodera glycines. This technique takes advantage of density changes that occur during embryogenesis in the developing embryo and egg to partition the egg within a sucrose step gradient. Sorted samples of eg...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2003.12.007
更新日期:2003-11-01 00:00:00
abstract::Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecifi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90130-x
更新日期:1986-02-01 00:00:00