Trypanosoma evansi: detection of Trypanosoma evansi DNA in naturally and experimentally infected animals using TBR(1) & TBR(2) primers.

abstract：:Visceral Leishmaniasis is an endemic disease in Brazil caused by Leishmania infantum chagasi and its main vector species is the sand fly Lutzomyia longipalpis. Epidemiological studies have used conventional PCR techniques to measure the rate of infection of sand flies collected in the field. However, real-time PCR can...

journal_title：Experimental parasitology

authors： Bezerra-Vasconcelos DR,Melo LM,Albuquerque ÉS,Luciano MC,Bevilaqua CM

更新日期：2011-11-01 00:00:00

abstract：:Serine proteases have been identified as important molecules that are involved in many parasitic infections, and these molecules have also been suggested to play important roles in Trichinella spiralis infections. In the present study, the antigenic serine protease gene Ts-ADSp-7, which was screened from a cDNA librar...

journal_title：Experimental parasitology

authors： Gao H,Tang B,Bai X,Wang L,Wu X,Shi H,Wang X,Liu X,Liu M

更新日期：2018-12-01 00:00:00

abstract：:Amoebae of the genus Acanthamoeba are free-living protozoa that can cause granulomatous encephalitis and keratitis in humans. In this study, four clinical and three household dust isolates obtained in Vitória, Espírito Santo, Brazil were characterized by their morphological, genotypic, and physiological properties. Al...

journal_title：Experimental parasitology

authors： Duarte JL,Furst C,Klisiowicz DR,Klassen G,Costa AO

更新日期：2013-09-01 00:00:00

abstract：:Parasites of the Leishmania genus require for the growth and viability the de novo synthesis of specific sterols as such as episterol and 5-dehydroepisterol because cholesterol, which is abundant in their mammalian hosts, does not fulfill the parasite sterol requirements. Squalene synthase catalyzes the first committe...

journal_title：Experimental parasitology

authors： Rodrigues JC,Urbina JA,de Souza W

更新日期：2005-12-01 00:00:00

abstract：:The N-terminal region of the cathepsin D-like aspartic protease from the human filarial parasite Onchocerca volvulus was expressed as His-tag fusion protein. Light and electron microscopic immunohistology using antibodies against the recombinant protein showed labeling of lysosomes in the hypodermis and epithelia of t...

journal_title：Experimental parasitology

authors： Jolodar A,Fischer P,Büttner DW,Miller DJ,Schmetz C,Brattig NW

更新日期：2004-07-01 00:00:00

abstract：:According to the World Health Organization, lymphatic filariasis (LF), a mosquito-borne neglected tropical disease (NTD), should be eliminated as a public health concern by the end of 2020. To this end, the goals of the Global Programme to Eliminate Lymphatic Filariasis (GPELF) include interrupting transmission throug...

journal_title：Experimental parasitology

authors： Albuquerque AL,Araújo TA,Melo DCTV,Paiva MHS,Melo FL,Oliveira CMF,Ayres CFJ

更新日期：2020-08-01 00:00:00

abstract：:Calfluxin (CaFl), one of the gonadotropic hormones of Lymnaea stagnalis, stimulates the influx of Ca2+ into the mitochondria of the cells of the albumen gland, one of the accessory sex organs of the snail. This effect is suppressed in glands of noninfected snails by an agent (schistosomin) present in the hemolymph of ...

journal_title：Experimental parasitology

authors： de Jong-Brink M,Elsaadany MM,Boer HH

更新日期：1988-02-01 00:00:00

abstract：:Extracting genomic DNA of pathogenic agents from formalin-fixed specimens is inherently difficult. Storage of samples in formalin results in nucleic acid cross-linking and DNA fragmentation. In this study, DNA was extracted from 45 Giardia-positive stool samples stored in formalin and subjected to PCR amplification ta...

journal_title：Experimental parasitology

authors： Lee MF,Lindo JF,Auer H,Walochnik J

更新日期：2019-03-01 00:00:00

abstract：:The study of the encystation process of Entamoeba histolytica has been hampered by the lack of experimental means of inducing mature cysts in vitro. Previously we have found that cytoplasmic vesicles similar to the encystation vesicles of Entamoeba invadens are present in E. histolytica trophozoites only in amebas rec...

journal_title：Experimental parasitology

authors： Segovia-Gamboa NC,Talamás-Rohana P,Ángel-Martínez A,Cázares-Raga FE,González-Robles A,Hernández-Ramírez VI,Martínez-Palomo A,Chávez-Munguía B

更新日期：2011-09-01 00:00:00

abstract：:Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...

journal_title：Experimental parasitology

authors： Ramírez JD,Duque MC,Montilla M,Cucunubá Z,Guhl F

更新日期：2012-12-01 00:00:00

abstract：:The yellow fever mosquito, Aedes aegypti, expresses a gene, Apyrase (Apy), that encodes an ATP-diphosphohydrolase. The product of this gene is a secreted enzyme that facilitates hematophagy by preventing platelet aggregation in the host. Apy gene expression is limited to the cells of the distal-lateral and medial lobe...

journal_title：Experimental parasitology

authors： Smartt CT,Kim AP,Grossman GL,James AA

更新日期：1995-11-01 00:00:00

abstract：:At least eight protein members of HSP70 and three of the HSP60 families have been identified in Trypanosoma cruzi; of these, five HSP70 isoforms and one HSP60 isoform were respectively induced by a 2-hr heat-shock treatment at 37 degrees C. Immunoelectronmicroscopy of epimastigote, spheromastigote, and metacyclic cell...

journal_title：Experimental parasitology

authors： Giambiagi-deMarval M,Souto-Padrón T,Rondinelli E

更新日期：1996-08-01 00:00:00

abstract：:Rhoptries are involved in host cell invasion and rhoptry polypeptides, including the Babesia bigemina rhoptry-associated protein-1 (RAP-1), are targets for protective immune responses. Polyclonal antisera produced against isolated rhoptries is directed predominantly against RAP-1 and reacts with both the merozoite and...

journal_title：Experimental parasitology

authors： Vidotto O,McElwain TF,Machado RZ,Perryman LE,Suarez CE,Palmer GH

更新日期：1995-12-01 00:00:00

abstract：:The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...

journal_title：Experimental parasitology

authors： Yoder BJ,Goodrum KJ

更新日期：2001-06-01 00:00:00

abstract：:Whole cell extracts of 10 clones of bloodstream forms of African trypanosomes representing two strains of Trypanosoma brucei gambiense, one strain of T. b. rhodesiense and one strain of T. b. brucei were fractionated on sodium dodecyl sulfate-polyacrylamide gels, electrophoretically transferred to nitrocellulose paper...

journal_title：Experimental parasitology

authors： Frommel TO,Balber AE

更新日期：1987-02-01 00:00:00

abstract：:Treatment with mannosidase or sialidase completely inhibited chemotactic responses of Caenorhabditis elegans wild type, C. elegans mutants CB1377 (daf-6)X and CB1379 (che-3)I, and Panagrellus redivivus to a source of attractants. Trypsin (EC3.4.21.4) caused a partial reduction in the level of chemoresponse. Normal che...

journal_title：Experimental parasitology

authors： Jansson HB,Jeyaprakash A,Damon RA Jr,Zuckerman BM

更新日期：1984-12-01 00:00:00

abstract：:A mode of action of the inducible treatment with trypsin for the development of Mesocestoides lineatus tetrathyridium to adult was analyzed by administering various agents effective on Ca2+-dependent metabolic pathways in the cells: protein kinase C activators such as a synthetic diacylglycerol, 1-oleoyl-2-acetylglyce...

journal_title：Experimental parasitology

authors： Kawamoto F,Fujioka H,Kumada N,Kojima K

更新日期：1986-12-01 00:00:00

abstract：:During its parasitic life stages, the marine ectoparasitic copepod Lepeophtheirus salmonis ingests large amounts of host blood, which contains high amounts of iron. Iron is an essential micronutrient, but also toxic in high dosages, and blood-feeding parasites like the salmon louse must thus possess an efficient syste...

journal_title：Experimental parasitology

authors： Tröße C,Kongshaug H,Dondrup M,Nilsen F

更新日期：2015-10-01 00:00:00

abstract：:Giardia lamblia is the most commonly detected parasite in the intestinal tract of humans and other mammals causing giardiasis. Giardia presents several cytoskeletal structures with microtubules as major components such as the ventral adhesive disk, eight flagella axonemes, the median body and funis. Many drugs have al...

journal_title：Experimental parasitology

authors： Mariante RM,Vancini RG,Melo AL,Benchimol M

更新日期：2005-05-01 00:00:00

abstract：:A monoclonal antibody specific for the Theileria parva sporozoite, which recognizes a determinant on the surface coat and blocks sporozoite infectivity, was used to investigate the presence of the determinant on other stages of the parasite lifecycle. Immunofluorescence techniques did not demonstrate this determinant ...

journal_title：Experimental parasitology

authors： Dobbelaere DA,Webster P,Leitch BL,Voigt WP,Irvin AD

更新日期：1985-08-01 00:00:00

abstract：:Only two drugs are currently available for the treatment of Chagas disease and their effectiveness are unsatisfactory. Photorhabdus luminescens and Xenorhabdus nematophila, two enteric bacteria highly pathogenic to a broad range of insects, have been studied as potential source for bioactive metabolites against protoz...

journal_title：Experimental parasitology

authors： Antonello AM,Sartori T,Silva MB,Prophiro JS,Pinge-Filho P,Heermann R,da Silva OS,Romão PRT

更新日期：2019-09-01 00:00:00

abstract：:There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...

journal_title：Experimental parasitology

authors： Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KD

更新日期：1995-11-01 00:00:00

abstract：:We show that a purified preparation of the prominent tartrate-resistant acid phosphatase (E.C.3.1.3.2), isolated from the external surface of the intracellular parasite Leishmania donovani (promastigote form), inhibits toxic oxidative metabolite production of neutrophils. Preincubation of a neutrophil suspension (2.5 ...

journal_title：Experimental parasitology

authors： Remaley AT,Glew RH,Kuhns DB,Basford RE,Waggoner AS,Ernst LA,Pope M

更新日期：1985-12-01 00:00:00

abstract：:We investigated the effects of the anti-malarials mefloquine and primaquine against the juvenile and adult life stages of Schistosoma mansoniin vitro. Cercariae were incubated with 0.5 μg/ml, 1 μg/ml and 2 μg/ml mefloquine or primaquine and with 1 μg/ml praziquantel for 12h. Schistosomula, pre-adults and adults were i...

journal_title：Experimental parasitology

authors： Holtfreter MC,Loebermann M,Klammt S,Sombetzki M,Bodammer P,Riebold D,Kinzelbach R,Reisinger EC

更新日期：2011-01-01 00:00:00

abstract：:Balamuthia mandrillaris is a free-living ameba (FLA) that has been isolated or its DNA identified in soil, dust and water. It causes a fatal central nervous system infection in humans and animals. Although it is environmental as Acanthamoeba and Naegleria fowleri, the two other free-living amebae that also cause CNS i...

journal_title：Experimental parasitology

authors： Yera H,Dupouy-Camet J,Jackson JW,Sriram R,Sweat S,Goldstein JM,Visvesvara GS

更新日期：2015-09-01 00:00:00

abstract：:Toxoplasma gondii, an intracellular parasite, has two distinctive growth stages, namely rapidly growing tachyzoites and slowly growing bradyzoites. Here we report a unique physiological function of the last committed glycolytic enzyme of T. gondii, lactate dehydrogenase (TgLDH), which is present in two isoforms and ex...

journal_title：Experimental parasitology

authors： Liwak U,Ananvoranich S

更新日期：2009-06-01 00:00:00

abstract：:The sphingolipids biosynthesis pathway generates bioactive molecules crucial to the regulation of physiological processes. We have recently reported that DAG (diacylglycerol) generated during sphingomyelin synthesis, plays an important role in PKC (protein kinase C) activation, necessary for the transit through the ce...

journal_title：Experimental parasitology

authors： Cerbon J,Olguin T,Alvarez-Grave PR,López-Sánchez RC

更新日期：2009-06-01 00:00:00

abstract：:Acanthamoeba are free-living amoebae found in most environments that can cause brain and corneal infections. To infect humans, these pathogens must interact with host cells and the extracellular matrix (ECM). In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyze...

journal_title：Experimental parasitology

authors： Rocha-Azevedo Bd,Jamerson M,Cabral GA,Marciano-Cabral F

更新日期：2010-09-01 00:00:00

abstract：:Parasites of wildlife inhabiting urbanised and peri-urban environments are of interest regarding wildlife population health, and also veterinary public health in the case of parasites that can also infect humans and domestic animals. This study aimed to: identify, and estimate the prevalence of, species of Eimeria par...

journal_title：Experimental parasitology

authors： Hillman AE,Yang R,Lymbery AJ,Thompson RCA

更新日期：2016-11-01 00:00:00

abstract：:In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more str...

journal_title：Experimental parasitology

authors： Cardona N,de-la-Torre A,Siachoque H,Patarroyo MA,Gomez-Marin JE

更新日期：2009-10-01 00:00:00