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Toxoplasma gondii: over-expression of lactate dehydrogenase enhances differentiation under alkaline conditions.

Abstract：

:Toxoplasma gondii, an intracellular parasite, has two distinctive growth stages, namely rapidly growing tachyzoites and slowly growing bradyzoites. Here we report a unique physiological function of the last committed glycolytic enzyme of T. gondii, lactate dehydrogenase (TgLDH), which is present in two isoforms and expressed in a stage-specific manner. TgLDH1 is present in tachyzoites while TgLDH2 is found in bradyzoites. Using clonal transgenic parasites over-expressing either TgLDH1 or TgLDH2, we showed that the enzymatic activity, growth, and virulence of tachyzoites were unaffected by the presence of the recombinant protein. Interestingly, under alkaline conditions the presence of the recombinant TgLDH proteins increased the differentiation, as detected by the formation of cyst structures in vitro, while green fluorescent protein did not. The differentiation enhancement of the recombinant TgLDH1 and TgLDH2 strongly suggests that TgLDH1 and TgLDH2 have an important physiological function, in addition to being glycolytic enzymes and differentiation markers

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Liwak U,Ananvoranich S

doi

10.1016/j.exppara.2009.01.016

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

155-61

issue

2

eissn

0014-4894

issn

1090-2449

journal_volume

122

pub_type

杂志文章

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