Abstract：
:Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Essential Oils (EO) obtained from leaves and flowers and aerial parts of Ammoides pusilla by an alternative method "Hydrodistillation''. Identification and quantification were realized by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography with Flame Ionization Detection (GC-FID). The main components of leaves and flowers and aerials parts were thymol (39.6% and 33.05%), γ-terpinene (28.97% and 28.19%), p-cymene (13.69% and 15.31%) and thymol methyl ether (7.33% and 8.91%), respectively. The antiparasitic activity of the EO was evaluated against Acanthamoeba castellanii Neff by the Alamar Blue® assay. Results showed that Ammoides pusilla amoebicidal activity from leaves and flowers essential oil (IC50 = 65.32 ± 5.43 μg/mL) was more important than those of aerial parts EO (IC50 = 97.18 ± 1.43 μg/ml).
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Souhaiel N,Sifaoui I,Ben Hassine D,Bleton J,Bonose M,Moussa F,Piñero JE,Lorenzo-Morales J,Abderrabba Mdoi
10.1016/j.exppara.2017.10.011subject
Has Abstractpub_date
2017-12-01 00:00:00pages
99-103eissn
0014-4894issn
1090-2449pii
S0014-4894(17)30430-7journal_volume
183pub_type
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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