Abstract：
:Under a variety of stress conditions, Leishmania species display some morphological and biochemical features characteristic of mammalian programmed cell death or necrosis. Nitroheteroaryl-1,3,4-thiadiazoles induce cell death in Leishmania major (L. major). Putative mechanisms of action of these compounds were investigated in vitro at cellular and molecular levels. We used colorimetric assay to measure acid phosphatase activity which is an indicator of cell viability in the promastigotes. The mode of toxicity was determined by detection of phosphatidylserine translocation to the surface, evaluation of cell membrane integrity, and in situ dUTP nick end-labeling assay. We also determined poly-ADP-ribose polymerase-like protein (PARP) level in the parasites after treatment. A significant reduction of acid phosphatase level, one of the most crucial and virulent factors of the parasite was found in parasites treated with 1,3,4-thiadiazole derivatives. In addition, 1,3,4-thiadiazole derivatives induced loss of plasma membrane integrity, DNA breakage, proteolysis of PARP and necrotic-like death in the parasites.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Ardestani SK,Poorrajab F,Razmi S,Foroumadi A,Ajdary S,Gharegozlou B,Behrouzi-Fardmoghadam M,Shafiee Adoi
10.1016/j.exppara.2012.06.002subject
Has Abstractpub_date
2012-10-01 00:00:00pages
116-22issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(12)00189-0journal_volume
132pub_type
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