Abstract:
:This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating among Didelphis albiventris as reservoir host in Brazil while heterogeneous populations were found in different regions of Colombia. KP1(+) minicircles were found in 100% isolates from Brazil and in 36.4% of the Colombian samples, whereas the KP2 and KP3 minicircles were detected in both groups. RAPD-PCR and LSSP-PCR profiles revealed a polymorphism within KP1(+) and KP1(-) T. rangeli populations and allowed the division of T. rangeli in two branches. The Brazilian KP1(+) isolates were more homogenous than the KP1(+) isolates from Colombia. The RAPD-PCR were entirely consistent with the distribution of KP1 minicircles while those obtained by LSSP-PCR were associated in 88.9% and 71.4% with KP1(+) and KP1(-) populations, respectively.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Marquez DS,Ramírez LE,Moreno J,Pedrosa AL,Lages-Silva Edoi
10.1016/j.exppara.2007.03.007subject
Has Abstractpub_date
2007-09-01 00:00:00pages
35-42issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(07)00081-1journal_volume
117pub_type
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