Abstract：
:In this work we demonstrated that promastigotes of Leishmania amazonensis exhibit an Mg-dependent ecto-ATPase activity, which is stimulated by heat shock. The Mg-dependent ATPase activity of cells grown at 22 and 28 degrees C was 41.0+/-5.2 nmol Pi/h x 10(7)cells and 184.2+/-21.0 nmol Pi/h x 10(7)cells, respectively. When both promastigotes were pre-incubated at 37 degrees C for 2h, the ATPase activity of cells grown at 22 degrees C was increased to 136.4+/-10.6 nmol Pi/h x 10(7) whereas that the ATPase activity of cells grown at 28 degrees C was not modified by the heat shock (189.8+/-10.3 nmol Pi/h x 10(7)cells). It was observed that Km of the enzyme from cells grown at 22 degrees C (Km=980.2+/-88.6 microM) was the same to the enzyme from cells grown at 28 degrees C (Km=901.4+/-91.9 microM). In addition, DIDS (4,4'-diisothiocyanatostilbene 2,2'-disulfonic acid) and suramin, two inhibitors of ecto-ATPases, also inhibited similarly the ATPase activities from promastigotes grown at 22 and 28 degrees C. We also observed that cells grown at 22 degrees C exhibit the same ecto-phosphatase and ecto 3'- and 5'-nucleotidase activities than cells grown at 28 degrees C. Interestingly, cycloheximide, an inhibitor of protein synthesis, suppressed the heat-shock effect on ecto-ATPase activity of cells grown at 22 degrees C were exposed at 37 degrees C for 2h. A comparison between the stimulation of the Mg-dependent ecto-ATPase activity of virulent and avirulent promastigotes by the heat shock showed that avirulent promastigotes had a higher stimulation than virulent promastigotes after heat stress.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Peres-Sampaio CE,de Almeida-Amaral EE,Giarola NL,Meyer-Fernandes JRdoi
10.1016/j.exppara.2008.01.003subject
Has Abstractpub_date
2008-05-01 00:00:00pages
135-43issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(08)00008-8journal_volume
119pub_type
杂志文章abstract：:Tapeworms Taenia solium and Taenia saginata are the causative agents of taeniasis/cysticercosis. These are diseases with high medical and veterinary importance due to their impact on public health and rural economy in tropical countries. The re-emergence of T. solium as a result of human migration, the economic burden...
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.09.018
更新日期：2012-12-01 00:00:00
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doi：10.1016/j.exppara.2011.06.002
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pub_type： 杂志文章,评审
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更新日期：2007-11-01 00:00:00
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doi：10.1016/j.exppara.2005.08.001
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0018
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journal_title：Experimental parasitology
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