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Chryseobacterium indologenes and Chryseobacterium gleum interact and multiply intracellularly in Acanthamoeba castellanii.

Abstract：

:Chryseobacterium indologenes and Chryseobacterium gleum are Gram negative environmental bacteria that have been frequently reported to implicate in fatal nosocomial infections, such as bacteraemia and ventilator-associated pneumonia in immunocompromised individuals in the past decades. The interaction between Chryseobacterium spp. and Acanthamoeba castellanii, a free-living amoeba ubiquitous in the environment, has not been explored previously. In this study, C. indologenes and C. gleum were co-cultured with A. castellanii trophozoites and their interactions were evaluated. Our results showed that when co-cultured with A. castellanii, bacterial numbers of C. indologenes and C. gleum increased significantly (p < 0.05), indicating growth-supporting role of A. castellanii. Specifically, our findings showed that C. indologenes and C. gleum were able to associate, invade and/or taken up by A. castellani trophozoites, and multiply intracellularly at similar rates (p > 0.05). Interestingly, the two Chryseobacterium spp. associated, invaded and/or taken up by A. castellanii at significantly higher rates than Escherichia coli K1, a neuropathogenic bacterial strain known to interact and replicate intracellularly in A. castellanii (p < 0.05). However, the ability of both Chryseobacterium spp. to multiply in A. castellanii was significantly weaker than E. coli K1 (p < 0.001). This is the first time that Chryseobacterium spp. and A. castellanii were shown to interact with each other. The ability to survive intracellularly in A. castellanii may confer protection to C. indologenes and C. gleum and assist in the survival and transmission of Chryseobacterium spp. to susceptible hosts within a hospital setting. Future studies will determine the ability of C. indologenes and C. gleum survival in A. castellanii cysts and the possible molecular mechanisms involved in such interactions.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Lim WG,Tong T,Chew J

doi

10.1016/j.exppara.2020.107862

subject

Has Abstract

pub_date

2020-02-19 00:00:00

pages

107862

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(19)30435-7

journal_volume

211

pub_type

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Chryseobacterium indologenes and Chryseobacterium gleum interact and multiply intracellularly in Acanthamoeba castellanii.