Abstract：
:A monoclonal antibody specific for the Theileria parva sporozoite, which recognizes a determinant on the surface coat and blocks sporozoite infectivity, was used to investigate the presence of the determinant on other stages of the parasite lifecycle. Immunofluorescence techniques did not demonstrate this determinant on the kinete, schizont, merozoite, or piroplasm stages of the parasite. Immunoautoradiography, using a tritiated form of the monoclonal antibody, on sections of infected salivary glands collected from ticks that had fed for 0, 1, 2, 3, or 4 days revealed that the determinant recognized was synthesized predominantly during sporogony, between 2 to 3 days after the tick started feeding. Immunoelectron microscopy was performed on ultrathin frozen sections of infected tick salivary glands incubated with the monoclonal antibody followed by Protein-A--colloidal gold. The antigen or its precursor could be detected in the developing parasite. In ticks fed 2 days, the sporoblast was labeled, both in the cytoplasm and on parasite membranes, often including the nuclear envelope. In sections from ticks fed 4 days, the sporozoite surface membrane was labeled, as were membrane-bounded sporozoite organelles identified as micronemes. Observation by immunofluorescence, on sporozoites incubated with bovine peripheral blood lymphocytes, suggested that the antigen recognized by the monoclonal antibody does not enter the lymphocyte during sporozoite endocytosis. We conclude that synthesis of the antigen or its precursor(s) occurs during sporogony in the feeding tick, at the time of maximal parasite proliferation, and precedes the formation of morphologically mature sporozoites; the antigen's role in the parasite life cycle also appears to be limited to events associated with the sporozoite entry process.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Dobbelaere DA,Webster P,Leitch BL,Voigt WP,Irvin ADdoi
10.1016/s0014-4894(85)80026-6subject
Has Abstractpub_date
1985-08-01 00:00:00pages
90-100issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(85)80026-6journal_volume
60pub_type
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更新日期：1993-12-01 00:00:00
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更新日期：1986-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：1994-11-01 00:00:00
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更新日期：2015-11-01 00:00:00
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