Abstract:
:The transport of the megadalton protein Pf332 was studied during the asexual bloodstage development of Plasmodium falciparum. Four mouse monoclonal antibodies, produced against a recombinant polypeptide derived from the Pf332 protein, were used to analyze the kinetics of synthesis, the subcellular location, and transport of this giant molecule to the erythrocyte membrane. After parasite invasion of a red blood cell, the Pf332 antigen is first detected in young trophozoites at the parasitophorous vacuole membrane or in the cytoplasm of the erythrocyte as large vesicle-like structures. The number of vesicles increases during maturation of the parasite and thus forms a rim-like immunofluorescence pattern between the erythrocyte membrane and the parasitophorous vacuole at very late stages. The various anti-Pf332 antibodies react with the surface of erythrocytes infected with very mature parasites (segmenter stage 42-46 hr postinvasion). Immunoelectron microscopic analysis shows that the Pf332 antigen is transported in association with Maurer's clefts in the cytoplasm of the erythrocyte. This transport could be completely blocked by Brefeldin A, resulting in the accumulation of the antigen within the parasite. These data strongly suggest that the Pf332 antigen is exported to the erythrocyte cytoplasm via the classical Golgi secretory pathway.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Hinterberg K,Scherf A,Gysin J,Toyoshima T,Aikawa M,Mazie JC,da Silva LP,Mattei Ddoi
10.1006/expr.1994.1091subject
Has Abstractpub_date
1994-11-01 00:00:00pages
279-91issue
3eissn
0014-4894issn
1090-2449pii
S0014489484710915journal_volume
79pub_type
杂志文章abstract::Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in bo...
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abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
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journal_title:Experimental parasitology
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pub_type: 杂志文章
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.01.009
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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doi:10.1016/0014-4894(91)90096-f
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journal_title:Experimental parasitology
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pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::The protozoan parasite Trypanosoma cruzi, the etiologic agent of Chagas disease, is an obligate intracellular protozoan pathogen. Overlapping mechanisms ensure successful infection, yet the relationship between these cellular events and clinical disease remains obscure. This review explores the process of cell invasio...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
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更新日期:2010-11-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90151-l
更新日期:1991-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.01.009
更新日期:2018-02-01 00:00:00
abstract::We probed DNA from all trypanosomatid genera by slot blot hybridization with an oligonucleotide (SL3') complementary to a sequence of the Phytomonas spliced-leader or mini-exon RNA. The 19-nucleotide probe target site was previously shown to be highly conserved among a limited number of Phytomonas isolates, but diverg...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0119
更新日期:1996-12-01 00:00:00
abstract::The N-terminal region of the cathepsin D-like aspartic protease from the human filarial parasite Onchocerca volvulus was expressed as His-tag fusion protein. Light and electron microscopic immunohistology using antibodies against the recombinant protein showed labeling of lysosomes in the hypodermis and epithelia of t...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2004.06.006
更新日期:2004-07-01 00:00:00