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Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba.

Abstract：

:Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana, Cuba between 2010 and 2013, with the aim of comparing the results from molecular (PCR) approaches targeting different genes in order to assign with confidence 224 isolates of G. duodenalis to the correct assemblages. In each sub-study, following DNA isolation by the phenol/chloroform/isoamyl alcohol extraction method, PCR targeting the triose phosphate isomerase (tpi) gene was used for molecular characterization, as well as one additional PCR-method targeting another gene or pair of genes. DNA amplification was obtained in 87%, 83%, and 80% in the three sub-studies. Although excellent agreement (kappa index = 1) was recorded between results from some pairs of genes, for other combinations only moderate or substantial agreement was achieved. These results highlight the importance of interpretation of genotyping data, especially when single genetic markers are used. From the results of our studies, PCR targeting a combination of the tpi gene and the intergenic spacer region of rDNA may be a useful approach for the molecular characterization of G. duodenalis isolates.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Jerez Puebla LE,Núñez Fernández FA,Fraga J,Rivero LR,Millán IA,Valdés LA,Silva IM,Müller N,Robertson LJ

doi

10.1016/j.exppara.2019.107814

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

107814

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(19)30405-9

journal_volume

209

pub_type

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