Abstract:
:In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus specific primer pair. The pathogenic potential of Acanthamoeba isolates was characterized using PCRs with two primer pairs related to Acanthamoeba pathogenesis and cytotoxicity assays using human corneal cells. Isolates genotypes were also determined after ribosomal DNA sequencing. These data revealed that the potentially pathogenic isolates belonged to T3 and T4. To our knowledge, this is the first report presenting the isolation of potentially pathogenic Acanthamoeba strains in wild squirrels, and it is also the first description of the pathogenic T3 and T4 Acanthamoeba genotypes in Fuerteventura and Morocco.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Lorenzo-Morales J,López-Darias M,Martínez-Carretero E,Valladares Bdoi
10.1016/j.exppara.2007.03.014subject
Has Abstractpub_date
2007-09-01 00:00:00pages
74-9issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(07)00088-4journal_volume
117pub_type
杂志文章abstract::Leishmania parasites lack catalase and therefore, their anti-oxidant system hinges primarily upon non-protein thiols; accordingly, depletion of thiols could potentially serve as an effective drug target. We have developed a flow cytometry based assay using 5-chloromethyl fluorescein diacetate based upon its selective ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.04.012
更新日期:2009-08-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
abstract::We have studied inflammatory reactions in the mesenteric tissue of mice infected with Schistosoma mansoni. Perivascular tissue contained diffuse infiltrates of macrophages, eosinophilic granulocytes and lymphocytes. Angiogenesis in the perivascular adipose tissue was associated with superficial plasmacytogenic foci. P...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90203-m
更新日期:1992-06-01 00:00:00
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journal_title:Experimental parasitology
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更新日期:1992-09-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1064
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::Large numbers (10(4) to greater than 10(5)/ml) of Type I water-resistant Giardia lamblia cysts were produced in vitro under conditions that are characteristic of the human intestinal lumen. We define Type I cyst morphology as oval shaped, smooth, and refractile, with cyst wall, axostyle, and median body visible in rel...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90185-9
更新日期:1989-08-01 00:00:00
abstract::This study examines the effects of ricinoleic acid esters from Ricinus communis castor oil on the vitellogenesis of Rhipicephalus sanguineus ticks attached to hosts that were fed with commercial rabbit food containing these esters. The oocytes of ticks from the treatment group (TG) showed cytoplasmic changes that inhi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.10.006
更新日期:2011-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1030
更新日期:1994-05-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1986-10-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90116-1
更新日期:1989-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.10.012
更新日期:2016-12-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.02.013
更新日期:2007-08-01 00:00:00
abstract::Aspartic proteases are important virulence factors for pathogens and are recognized as attractive drug targets. Seven aspartic proteases (ASPs) have been identified in Toxoplasma gondii genome. Bioinformatics and phylogenetic analyses regroup them into five monophyletic groups. Among them, TgASP1, a coccidian specific...
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pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
abstract::The aim of this study was to evaluate the effects of selenium and copper on oxidative stress and its performance in lambs experimentally infected with Haemonchus contortus. Twenty-eight five-months old lambs were experimentally infected by the oral route with 5000 third-stage infective larvae and allocated into four g...
journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
abstract::Visceral Leishmaniasis is an endemic disease in Brazil caused by Leishmania infantum chagasi and its main vector species is the sand fly Lutzomyia longipalpis. Epidemiological studies have used conventional PCR techniques to measure the rate of infection of sand flies collected in the field. However, real-time PCR can...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.08.010
更新日期:2011-11-01 00:00:00
abstract::M17 leucine aminopeptidase (LAP) is one of a family of metalloexopeptidases, of which short peptide fragments are cleaved from the N-terminals. In this study, the full length of cDNA encoding Fasciola gigantica LAP (FgLAP) was cloned from adult parasites. The amino acid sequences of FgLAP showed a high degree of ident...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.04.008
更新日期:2012-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.09.013
更新日期:2007-03-01 00:00:00
abstract::Interleukin-13 (IL-13) is a powerful anti-inflammatory cytokine that was previously shown to be a susceptibility factor for Leishmania major (L. major) infection. In this study we report a different role for IL-13 in rats infected with L. major; rIL-13 stimulates expression of pro-inflammatory cytokines and IL-12 whic...
journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2009-03-01 00:00:00
abstract:PURPOSE:Praziquantel (PZQ) is the conventional antibilharzial agent. Nevertheless, no antibilharzial prophylactic agents or 100% curable therapy approved and no reported data about use of human CD34+ Umbilical Cord Blood Stem Cells (CD34+UCBSCs) or Wharton Jelly Mesenchymal Stem Cells (WJMSCs) in prevention and/or comp...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
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更新日期:2020-10-01 00:00:00
abstract::Giardia lamblia undergo surface antigenic variation in vitro and in vivo. The presence of variant trophozoites can be detected in clones after exposure to cytotoxic monoclonal antibodies. Surviving Giardia (progeny) no longer possess the initial major surface antigen which is replaced by new antigens. Exposure of a cl...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/0014-4894(89)90104-5
更新日期:1989-02-01 00:00:00
abstract::Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplifica...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1108
更新日期:1993-12-01 00:00:00
abstract::The goal of this study was to compare two parasitological diagnostic techniques, such as by Micro-Haematocrit Centrifugation Technique (MHCT) and Direct Microscopic Examination (DME) with a serological method (iELISA), and a molecular procedure PCR, in rabbits experimentally infected with Trypanosoma evansi, in order ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.02.010
更新日期:2011-05-01 00:00:00