Abstract:
:This report describes a molecular method for determining in a first step the generic composition of a nematode community and in a second step, the resistance of each species to benzimidazole (BZ). We first established a polymerase chain reaction (PCR) linked to a restriction fragment length polymorphism strategy using the isotype 1 beta-tubulin gene. This method overcame the limitations of morphological identification of larval stages of trichostrongylid nematode species. Geographically distant isolates from the three main gastrointestinal species in temperate zones, Teladorsagia circumcincta, Haemonchus contortus, and Trichostrongylus colubriformis, were distinguished using this method. We then used an allele-specific PCR (AS-PCR) to detect mutations of residue 200 of the beta-tubulin, which is implicated in BZ resistance. The sequences of several samples confirmed the BZ-resistance genotype determined by AS-PCR. The ability to process large numbers of samples simultaneously makes this PCR-based strategy particularly suitable for epidemiological studies. It may also be useful for monitoring the emergence of resistant alleles in nematode communities.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Silvestre A,Humbert JFdoi
10.1006/expr.2000.4542keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
271-6issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(00)94542-9journal_volume
95pub_type
杂志文章abstract:PURPOSE:Praziquantel (PZQ) is the conventional antibilharzial agent. Nevertheless, no antibilharzial prophylactic agents or 100% curable therapy approved and no reported data about use of human CD34+ Umbilical Cord Blood Stem Cells (CD34+UCBSCs) or Wharton Jelly Mesenchymal Stem Cells (WJMSCs) in prevention and/or comp...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/j.exppara.2020.107938
更新日期:2020-10-01 00:00:00
abstract::A Schistosoma mansoni G-protein coupled receptor (SmGPCR) was previously cloned and shown to be activated by the biogenic amine, histamine. Here we report a first investigation of the receptor's subunit organization, tissue distribution and expression levels in different stages of the parasite. A polyclonal antibody w...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.01.001
更新日期:2009-05-01 00:00:00
abstract::Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different classes of drugs, which were described to produce unpleasant side effects. Mentha x piperita, popularly known as peppermint, is a plant that is frequently used in the popular medicine to treat gast...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.05.001
更新日期:2007-01-01 00:00:00
abstract::Shiraz district in south of Iran is a classical focus of Cutaneous leishmaniasis (CL) and previous research has consistently documented the etiologic agent to be Leishmania tropica and Leishmania major in urban and rural areas, respectively. However, none of the Phlebotomus sergenti, a known vector for L. tropica, of ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.05.020
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Among different leishmanial infections, visceral leishmaniasis (VL) if not treated is the most severe form with high mortality rates. In India, it is caused by the protozoan parasite Leishmania donovani. The therapy of visceral leishmaniasis is limited due to high toxicity, resistance to existing drugs and i...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.07.018
更新日期:2018-10-01 00:00:00
abstract::Gastro-intestinal (GI) parasites are of great agricultural importance, annually costing the livestock industry vast amounts in resources to control parasitism. One such GI parasite, Haemonchus contortus, is principally pathogenic to sheep; with the parasite's blood-feeding behaviour causing effects ranging from mild a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.08.001
更新日期:2005-12-01 00:00:00
abstract::Six simple methods for extraction of ribosomal and mitochondrial DNA from Toxocara canis, Toxocara cati and Toxascaris leonina were compared by evaluating the presence, appearance and intensity of PCR products visualized on agarose gels and amplified from DNA extracted by each of the methods. For each species, two iso...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.02.008
更新日期:2013-06-01 00:00:00
abstract::Previous results from our laboratory using pharmacological approaches suggested a role for nitric oxide (NO) in the host defense against the human filarial parasite, Brugia malayi. We sought to determine whether a complementary genetic approach, using mice homozygous for a targeted mutation in the gene encoding induci...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2001.4611
更新日期:2001-05-01 00:00:00
abstract::Acanthamoeba infections cause serious humans diseases, such as amoebic keratitis and granulomatous amoebic encephalitis. Melaleuca essential oil has been reported to be effective in treating bacterial and fungal infections. However, the anti-parasitic effects of this essential oil are not well studied. In the present ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2017.10.014
更新日期:2017-12-01 00:00:00
abstract::The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1110
更新日期:1994-12-01 00:00:00
abstract::The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2001.4622
更新日期:2001-06-01 00:00:00
abstract::The contribution of mononuclear phagocytes to host resistance against acute Chagas disease has been studied in vivo in a rat model inoculated with Trypanosoma cruzi, Y strain. Acute T. cruzi infection triggered a dramatic increase (937%) in peripheral blood monocyte number at day 12 of infection. At this point, histol...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2000.4576
更新日期:2001-01-01 00:00:00
abstract::Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as ass...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.09.012
更新日期:2012-01-01 00:00:00
abstract::The present study aimed to evaluate the acaricidal efficacy of fluazuron (2.5 mg/kg), administered as a pour-on, in comparison to an injectable formulation containing fluazuron (1.6 mg/kg) + ivermectin (0.63 mg/kg), against Rhipicephalus (Boophilus) microplus in naturally and experimentally infested cattle. Two studie...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.02.004
更新日期:2015-06-01 00:00:00
abstract::A drug-resistant Trypanosoma congolense strain with predetermined curative doses (CD50 and CD90) of samorin at 13.9 +/- 1.02 and 20.3 +/- 1.13 mg/kg body weight, respectively, was cyclically transmitted through tsetse flies and by syringe passages in mice in the absence of drug pressure. The changing levels of drug se...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90085-4
更新日期:1989-11-01 00:00:00
abstract::Phlebotomus papatasi is susceptible to Leishmania major which it transmits in nature, but is resistant to L. donovani. The present study compares the effect of L. major and L. donovani on the proteolytic activity of P. papatasi gut enzymes. The experiments measured digestion of C14-labeled globin by gut homogenates of...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90045-7
更新日期:1986-12-01 00:00:00
abstract::Chemical acaricides, especially fipronil (active ingredient of Frontline®), are still the most effective method to control tick populations. In this study, the effectiveness of fipronil was assessed in semi-engorged females of the tick Rhipicephalus sanguineus. A protocol for an in vitro bioassay (AIT) was developed, ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.09.009
更新日期:2011-02-01 00:00:00
abstract::It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.02.013
更新日期:2007-08-01 00:00:00
abstract::Stable RNAs have regions of primary sequence that are nearly identical in every member of the Plasmodium genus and not found in the host or in other common pathogens. Several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal RNA of Plasmodium species. Pr...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1107
更新日期:1995-09-01 00:00:00
abstract::The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.03.002
更新日期:2018-04-01 00:00:00
abstract::Although macrophages have a microbicidal role in the immune system they themselves can be infected by pathogens. Often a simultaneous infection by more than one microbe may occur in a single cell. This is the first report of coinfection of macrophages with Toxoplasma gondii and Leishmania infantum, in vitro and in viv...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.02.022
更新日期:2011-07-01 00:00:00
abstract::Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stool...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.03.006
更新日期:2008-07-01 00:00:00
abstract::Kinetoplastid organisms possess a unique organelle, the glycosome, which compartmentalizes the Embden-Meyerhof segment of glycolysis and several other metabolic pathways. In Trypanosoma brucei many of the enzyme activities localized to the glycosome are stage regulated. Two-dimensional gel analysis was used to examine...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(90)90109-p
更新日期:1990-04-01 00:00:00
abstract::In this paper, the lytic activity of two variants of Serratia marcescens against promastigotes of Leishmania braziliensis was studied. In vitro assays showed that S. marcescens variant SM365 lyses L. braziliensis promastigotes, while the variant DB11 did not. Scanning electron microscopy (SEM) revealed that S. marcesc...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.03.004
更新日期:2009-06-01 00:00:00
abstract::Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.09.017
更新日期:2012-12-01 00:00:00
abstract::In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.03.014
更新日期:2007-09-01 00:00:00
abstract::Trichinella spiralis (T. spiralis) muscle larvae (ML) excretory/secretory products (ESPs) are antitumor substances extracted from the culture medium of T. spiralis ML. The ESPs inhibit tumor growth and induce tumor cell apoptosis. To explore the effects of these products on the non-small-cell lung cancer (NSCLC) line ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2020.107983
更新日期:2020-11-01 00:00:00
abstract::Phospholipid metabolism of the microsporidian Encephalitozoon cuniculi, an obligate intracellular parasite, has been investigated. Labeled precursor incorporation experiments have shown that phosphatidylserine decarboxylase and phosphatidylethanolamine N-methyltransferase are more active in cells infected by E. cunicu...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2001.4635
更新日期:2001-08-01 00:00:00
abstract::Whole cell extracts of 10 clones of bloodstream forms of African trypanosomes representing two strains of Trypanosoma brucei gambiense, one strain of T. b. rhodesiense and one strain of T. b. brucei were fractionated on sodium dodecyl sulfate-polyacrylamide gels, electrophoretically transferred to nitrocellulose paper...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(87)90075-0
更新日期:1987-02-01 00:00:00
abstract::Chlorination is a well-known disinfection method, used in water treatment to inactivate various microorganisms, it induces numerous cellular changes. Even though Acanthamoebae are frequently found in water, the cellular changes induced in Acanthamoebae have not been described in the literature. Acanthamoebae are patho...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.12.005
更新日期:2010-09-01 00:00:00