Abstract：
:Through a continuous in vivo drug pressure protocol, using mice as experimental model, we induced benznidazole resistance in Trypanosoma cruzi stocks. Full resistance was obtained for four out of five T. cruzi stocks analyzed. However, the number of benznidazole doses (40-180), as well as the time (4-18 months) necessary to induce resistance varied among the different T. cruzi stocks. The resistance phenotype remained stable after T. cruzi stocks has been maintained by 12 passages in mice (six months) and in acellular culture for the same time. However, the maintenance of resistant parasite for 12 months in acellular culture induces a reduction in its level of benznidazole resistance, while no alteration was detected in parasite maintained for the same time in mice. The data showed the stability of the resistance acquired by drug pressure, but suggest the possibility of reversible changes in the resistance levels after maintenance for long time in acellular culture.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Dos Santos FM,Caldas S,de Assis Cáu SB,Crepalde GP,de Lana M,Machado-Coelho GL,Veloso VM,Bahia MTdoi
10.1016/j.exppara.2008.09.007subject
Has Abstractpub_date
2008-12-01 00:00:00pages
385-90issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(08)00226-9journal_volume
120pub_type
杂志文章abstract：:Stable RNAs have regions of primary sequence that are nearly identical in every member of the Plasmodium genus and not found in the host or in other common pathogens. Several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal RNA of Plasmodium species. Pr...
journal_title：Experimental parasitology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.12.006
更新日期：2005-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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pub_type： 杂志文章
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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