Neolignans from plants in northeastern Brazil (Lauraceae) with activity against Trypanosoma cruzi.

Abstract:

:Trypanosoma cruzi is the ethiological agent for Chagas disease in Latin America. This study aimed to test the trypanocidal effect of licarin A and burchellin isolated from plants in northeastern Brazil. These neolignans were tested on T. cruzi and on peritoneal macrophages, to evaluate drug toxicity. Epimastigote growth was inhibited in 45% with licarin A and 20% with burchellin with an IC(50)/96 h of 462.7 microM and 756 microM, respectively. Epimastigotes treated with licarin A presented swollen mitochondria and disorganized mitochondrial cristae, kDNA and Golgi complex. When treated with burchellin, they presented enormous autophagosomes and chromatin disorganization. Licarin A and burchellin were able to induce trypomastigote death with IC(50)/24 h of 960 microM and 520 microM, respectively. Although licarin A presented an IC(50) for trypomastigotes higher than for epimastigotes, both substances acted as therapeutic trypanocidal agents, because they were able to kill parasites without affecting macrophages. Due to our results, burchellin and licarin A need to be further analysed to observe if they may be used as alternative blood additive prophylaxis against Chagas disease, since it has been established that blood transfusion is an important mechanism in the transmission process.

journal_name

Exp Parasitol

authors

Cabral MM,Barbosa-Filho JM,Maia GL,Chaves MC,Braga MV,De Souza W,Soares RO

doi

10.1016/j.exppara.2009.11.007

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

319-24

issue

3

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(09)00311-7

journal_volume

124

pub_type

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