Abstract：
:Kinetoplastid organisms possess a unique organelle, the glycosome, which compartmentalizes the Embden-Meyerhof segment of glycolysis and several other metabolic pathways. In Trypanosoma brucei many of the enzyme activities localized to the glycosome are stage regulated. Two-dimensional gel analysis was used to examine the characteristics, expression, and biosynthesis of the major glycosomal proteins. Two-dimensional gel maps of glycosomes from slender bloodforms and late intermediate-stumpy bloodforms (the precursors of procyclic forms) were indistinguishable, while those of procyclic form glycosomes showed extensive differences. Glycosomal phosphoenolpyruvate carboxykinase and malate dehydrogenase were identified to have subunit molecular weights of 60 and 34 kDa, respectively. We detected two hitherto undescribed glycosomal proteins, one of which is found only in bloodforms. All of the major proteins, except glucose phosphate isomerase, were highly basic. Stage regulation of glycosomal enzyme activities correlated with stage regulation of specific protein biosynthesis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Parsons M,Nielsen Bdoi
10.1016/0014-4894(90)90109-psubject
Has Abstractpub_date
1990-04-01 00:00:00pages
276-85issue
3eissn
0014-4894issn
1090-2449pii
0014-4894(90)90109-Pjournal_volume
70pub_type
杂志文章abstract：:How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations > or = 10(-10) M. Motil...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1064
更新日期：1993-08-01 00:00:00
abstract：:Three-day-old specific-pathogen-free chickens were subcutaneously immunized with Eimeria tenella recombinant 5401 antigen (100 microg per chicken) with (0.25, 0.5 or 1.0mg per dose) or without ginsenosides, and boosted with the same dosage 14 days later. The chickens were challenged with 6 x 10(4) homologous sporulate...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.07.006
更新日期：2005-11-01 00:00:00
abstract：:This article describes a sensitive, cheap, and easy method for assaying chemotaxic responses of Leishmania promastigotes. A gradient of the substance to be assayed was produced inside a series of commercially available capillary tubes submerged in a promastigote suspension. After an incubation period, the attractivene...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2000.4569
更新日期：2000-11-01 00:00:00
abstract：:The chemical properties of the primary antileishmanial agent sodium stibogluconate (Pentostam), and the interaction of Pentostam with Leishmania mexicana amastigotes, have been investigated with the aid of [125Sb]Pentostam. The molecular weight by P2 chromatography showed [125Sb]Pentostam to be of multiple species of ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(88)90012-4
更新日期：1988-10-01 00:00:00
abstract：:Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.08.003
更新日期：2009-12-01 00:00:00
abstract：:Trypanosoma rangeli is a parasite of a numerous wild and domestic animals, presenting wide geographical distribution and high immunological cross-reactivity with Trypanosoma cruzi, which may lead to misdiagnosis. T. rangeli has a complex life cycle, involving distinct morphological and functional forms in the vector. ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.11.015
更新日期：2006-04-01 00:00:00
abstract：:The infection with blood stages of Plasmodium chabaudi chabaudi (AS) was followed in BALB/c and DBA/2 mice. Both strains show a peak parasitemia by 7-9 days after infection, display splenic hypercellularity of T and B cells, thymic atrophy, nearly complete depletion of B cells in the bone marrow, and mount comparable ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.03.024
更新日期：2005-08-01 00:00:00
abstract：:Leishmania parasites lack catalase and therefore, their anti-oxidant system hinges primarily upon non-protein thiols; accordingly, depletion of thiols could potentially serve as an effective drug target. We have developed a flow cytometry based assay using 5-chloromethyl fluorescein diacetate based upon its selective ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.04.012
更新日期：2009-08-01 00:00:00
abstract：:Infection with Leishmania infantum causes the disease visceral leishmaniasis (VL), which is a serious clinical and veterinary problem. The drugs used to treat canine leishmaniasis (CanL) do not cause complete parasite clearance; they can be toxic, and emerging drug resistance in parasite populations limits their clini...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2020.108033
更新日期：2021-01-01 00:00:00
abstract：:Toxoplasma gondii is an intra-cellular parasite that infects humans through vertical and horizontal transmission. The cysts remain dormant in the brain of infected humans and can reactivate in immunocompromised hosts resulting in acute toxoplasmic encephalitis which may be fatal. We determined the onset and progressio...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.10.004
更新日期：2012-01-01 00:00:00
abstract：:Recombinant allergens are currently the best option for serodiagnosis of human anisakiasis in terms of sensitivity and specificity. However, previous reports showed high rates of anisakiasis patients who were negative to Ani s 7 and especially to Ani s 1. Recently, Anisakis haemoglobin was described as a major allerge...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.08.010
更新日期：2017-10-01 00:00:00
abstract：:The aim of this study was to evaluate the activity of purinergic enzymes in lymphocytes and cardiac tissue of mice experimentally infected by Trypanosoma cruzi. Twelve female mice were used, divided into two groups (n = 6): uninfected and infected. On day 12 post-infection (PI), the animals were anesthetized and after...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.02.002
更新日期：2017-04-01 00:00:00
abstract：:The anthelmintic effects of plumbagin (PB, 5-hydroxy-2-methyl-1,4-naphthoquinone) and praziquantel (PZQ) against adult Schistosoma mansoni in vitro were compared by estimating the relative motility (RM) values, survival indices (SI) and alterations of the tegument of flukes incubated in M-199 medium containing 1, 10 a...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.10.003
更新日期：2013-01-01 00:00:00
abstract：:Philasterides dicentrarchi is a histophagous scuticociliate infecting Korean olive flounder farms in Jeju Island, South Korea, where it causes significant economic losses. However, the route of entry of these parasites in olive flounder is currently unknown. In the present study, we attempted experimental infection wi...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.12.007
更新日期：2012-03-01 00:00:00
abstract：:Leishmaniasis is one of the most neglected tropical infectious diseases in the world. The emergence of drug resistance and toxicity and the high cost of the available drugs with a lack of new anti-leishmanial drugs highlight the need to search for newer therapies with anti-leishmanial activities. Due to the mesenchyma...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2020.108063
更新日期：2021-01-04 00:00:00
abstract：:Spleen cells from BALB/c mice were fused with myeloma cells following infection of the mice with Trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. A monoclonal antibody (Mab), designated as TS 3G6 which did not react with sera or tissue extracts from noninfected mice, rats, and gu...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90018-r
更新日期：1991-08-01 00:00:00
abstract：:This study aimed to investigate the effects of diphenyl diselenide (PhSe)2 to treat mice experimentally infected by Toxoplasma gondii on seric biomarkers of cardiac function (creatine kinase, creatine kinase MB, troponin, and myoglobin), and lactate dehydrogenase, as well as to evaluate the enzymatic activity of creat...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.04.014
更新日期：2016-08-01 00:00:00
abstract：:High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.06.007
更新日期：2010-12-01 00:00:00
abstract：:The intestine of Haemonchus contortus is hypersensitive to the effects of the anthelmintic fenbendazole (FBZ). The effects are postulated to stem from disruption of microtubules and interference with apical secretory vesicle transport, followed by release of digestive enzymes into the intestinal cell cytoplasm. Here, ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(02)00135-2
更新日期：2002-08-01 00:00:00
abstract：:Entomopathogenic nematodes complete their life cycles inside dead insects. The emergence of new infective juveniles from the cadaver has been attributed (but never demonstrated) to food depletion or to the accumulation of metabolites from the breakdown of the host's tissues. Here we give evidence that emergence is tri...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.01.008
更新日期：2008-05-01 00:00:00
abstract：:Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.05.006
更新日期：2016-08-01 00:00:00
abstract：:Trichinella spiralis (T. spiralis) muscle larvae (ML) excretory/secretory products (ESPs) are antitumor substances extracted from the culture medium of T. spiralis ML. The ESPs inhibit tumor growth and induce tumor cell apoptosis. To explore the effects of these products on the non-small-cell lung cancer (NSCLC) line ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2020.107983
更新日期：2020-11-01 00:00:00
abstract：:Propylene glycol used as an ophthalmic demulcent in certain contact-lens care systems has been included recently among factors responsible for increasing Acanthamoeba keratitis. In this study, we provide evidence that propylene glycol as well as examined contact-lens solutions containing it induce rapid differentiatio...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.08.014
更新日期：2011-01-01 00:00:00
abstract：:A monoclonal antibody specific for the Theileria parva sporozoite, which recognizes a determinant on the surface coat and blocks sporozoite infectivity, was used to investigate the presence of the determinant on other stages of the parasite lifecycle. Immunofluorescence techniques did not demonstrate this determinant ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(85)80026-6
更新日期：1985-08-01 00:00:00
abstract：:Feeding behavior and some antihemostatic properties of the saliva of Rhodnius prolixus infected with Trypanosoma rangeli were studied and compared with those of uninfected controls. Insects with salivary gland infection pierced host skin more often and drew less blood and at a lower rate than controls when feeding on ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1030
更新日期：1994-05-01 00:00:00
abstract：:Cardiac beta-adrenergic receptors' function was studied in the acute phase of Chagas' disease in mice reinfected with Trypanosoma cruzi Tulahuen strain (Tul) and with parasites isolated from an infected patient (SGO-Z12). Genetic characterization of SGO-Z12 isolates demonstrated that it belongs to the zimodeme Z12, on...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(03)00096-1
更新日期：2003-03-01 00:00:00
abstract：:A series of novel hybrid 4-aminoquinoline 1,3,5-triazine derivatives was synthesized in a five-steps reaction and evaluated for their in vitro antimalarial activity against chloroquine-sensitive (3D7) and chloroquine-resistant (RKL-2) strains of Plasmodium falciparum. Entire synthetic derivatives showed higher antimal...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.06.016
更新日期：2015-10-01 00:00:00
abstract：:Microtus fortis is a non-permissive host for Schistosoma japonicum. While M. fortis lymphocytes are known to provide natural resistance against S. japonicum, the specific mechanism remains unclear. A bone marrow transplantation (BMT) model was established using immunodeficient mice, either nude (experiment 1) or V(D)J...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.04.005
更新日期：2014-07-01 00:00:00
abstract：:Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1103
更新日期：1994-11-01 00:00:00
abstract：:In order to investigate the factors determining the expulsion of intestinal trematodes, we have analyzed the in vivo cytokine responses at several levels and the local responses against Echinostoma caproni (Trematoda) in two host species displaying different compatibility with the parasite. The response of the high co...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.09.004
更新日期：2011-02-01 00:00:00
