Toxoplasma gondii: chimeric Dr fimbriae as a recombinant vaccine against toxoplasmosis.

abstract：:The anthelmintic effects of plumbagin (PB, 5-hydroxy-2-methyl-1,4-naphthoquinone) and praziquantel (PZQ) against adult Schistosoma mansoni in vitro were compared by estimating the relative motility (RM) values, survival indices (SI) and alterations of the tegument of flukes incubated in M-199 medium containing 1, 10 a...

journal_title：Experimental parasitology

authors： Lorsuwannarat N,Saowakon N,Ramasoota P,Wanichanon C,Sobhon P

更新日期：2013-01-01 00:00:00

abstract：:Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in bo...

journal_title：Experimental parasitology

authors： Jerez Puebla LE,Núñez Fernández FA,Fraga J,Rivero LR,Millán IA,Valdés LA,Silva IM,Müller N,Robertson LJ

更新日期：2020-02-01 00:00:00

abstract：:The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...

journal_title：Experimental parasitology

authors： Beck DL,Tanyuksel M,Mackey AJ,Haque R,Trapaidze N,Pearson WR,Loftus B,Petri WA

更新日期：2002-06-01 00:00:00

abstract：:Hyperinfective strongyloidiasis involving the threadworm , Strongyloides stercoralis, is well known in humans and primates. Although this nematode also frequently parasitizes dogs, canine hyperinfective strongyloidiasis has not been reported. To determine whether a fulminant pattern of nematode development can occur i...

journal_title：Experimental parasitology

authors： Schad GA,Hellman ME,Muncey DW

更新日期：1984-06-01 00:00:00

abstract：:The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F. hepatica, rats were treated with a single 100mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after ...

journal_title：Experimental parasitology

authors： Keiser J,Morson G

更新日期：2008-05-01 00:00:00

abstract：:Infection with Leishmania infantum causes the disease visceral leishmaniasis (VL), which is a serious clinical and veterinary problem. The drugs used to treat canine leishmaniasis (CanL) do not cause complete parasite clearance; they can be toxic, and emerging drug resistance in parasite populations limits their clini...

journal_title：Experimental parasitology

authors： Miret JA,Moreno J,Nieto J,Carter KC,Mullen AB,Ambros L,Rodríguez C,San Andrés MI,González F

更新日期：2021-01-01 00:00:00

abstract：:Six chimpanzees (Pan troglodytes) and six mangabey monkeys (Cercocebus atys) were inoculated with Onchocerca volvulus third-stage larvae (L3) of West African origin. Two chimpanzees each received 200, 300, or 400 L3, while three mangabeys each received either 50 or 250 L3. All six chimpanzees became microfilaria posit...

journal_title：Experimental parasitology

authors： Eberhard ML,Dickerson JW,Tsang VC,Walker EM,Ottesen EA,Chandrashekar R,Weil GJ,Trpis M,Strobert E,Constantinidis I

更新日期：1995-05-01 00:00:00

abstract：:A series of novel hybrid 4-aminoquinoline 1,3,5-triazine derivatives was synthesized in a five-steps reaction and evaluated for their in vitro antimalarial activity against chloroquine-sensitive (3D7) and chloroquine-resistant (RKL-2) strains of Plasmodium falciparum. Entire synthetic derivatives showed higher antimal...

journal_title：Experimental parasitology

authors： Bhat HR,Singh UP,Thakur A,Kumar Ghosh S,Gogoi K,Prakash A,Singh RK

更新日期：2015-10-01 00:00:00

abstract：:Leishmania parasites lack catalase and therefore, their anti-oxidant system hinges primarily upon non-protein thiols; accordingly, depletion of thiols could potentially serve as an effective drug target. We have developed a flow cytometry based assay using 5-chloromethyl fluorescein diacetate based upon its selective ...

journal_title：Experimental parasitology

authors： Sarkar A,Mandal G,Singh N,Sundar S,Chatterjee M

更新日期：2009-08-01 00:00:00

abstract：:Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C. sinensis. The primers designed from the nucleotide sequence data der...

journal_title：Experimental parasitology

authors： Parvathi A,Sanath Kumar H,Kenchanna Prakasha B,Lu J,Xu X,Hu W,Feng Z,Karunasagar I,Karunasagar I

更新日期：2007-03-01 00:00:00

abstract：:Through a continuous in vivo drug pressure protocol, using mice as experimental model, we induced benznidazole resistance in Trypanosoma cruzi stocks. Full resistance was obtained for four out of five T. cruzi stocks analyzed. However, the number of benznidazole doses (40-180), as well as the time (4-18 months) necess...

journal_title：Experimental parasitology

authors： Dos Santos FM,Caldas S,de Assis Cáu SB,Crepalde GP,de Lana M,Machado-Coelho GL,Veloso VM,Bahia MT

更新日期：2008-12-01 00:00:00

abstract：BACKGROUND:Among different leishmanial infections, visceral leishmaniasis (VL) if not treated is the most severe form with high mortality rates. In India, it is caused by the protozoan parasite Leishmania donovani. The therapy of visceral leishmaniasis is limited due to high toxicity, resistance to existing drugs and i...

journal_title：Experimental parasitology

authors： Singh N,Chauhan IS

更新日期：2018-10-01 00:00:00

abstract：:This study aimed to evaluate the susceptibility of Brazilian isolates of Trypanosoma evansi to suramin sodium. For this purpose, three isolates of T. evansi (LPV-2005, LPV-2009 and LPV-2010) and seventy mice were used, with the animals divided in 10 groups (A, B, C, D, E, F, G, H, I and J) with seven animals each grou...

journal_title：Experimental parasitology

authors： Faccio L,Da Silva AS,Gressler LT,Tonin AA,Lazzarotto CR,Miletti LC,Monteiro SG

更新日期：2013-07-01 00:00:00

abstract：:The anthelmintic fenbendazole (FBZ) induces nuclear DNA fragmentation (DF) in intestinal cells of Haemonchus contortus. The DNA fragments had 3'-OH, which suggests involvement of a neutral DNase. To identify candidate DNase(s) involved, DNase activity in H. contortus intestine and other worm fractions was characterize...

journal_title：Experimental parasitology

authors： Kwak D,Jasmer DP

更新日期：2003-07-01 00:00:00

abstract：:A polymerase chain reaction (PCR-based) assay was evaluated for detection of Trypanosoma evansi DNA in experimentally infected mice and naturally infected camels, sheep and goats using the set of primers TBr(1) & TBr(2) that amplified 164 bp DNA fragment. The results revealed that PCR-based assay was able to detect T....

journal_title：Experimental parasitology

authors： Ashour AA,Abou El-Naga TR,Barghash SM,Salama MS

更新日期：2013-05-01 00:00:00

abstract：:Susceptible A/J and more resistant C57BL/6J mice were infected with Plasmodium chabaudi chabaudi 54X, P.c. chabaudi AS and Plasmodium chabaudi adami 408XZ. As expected, most C57BL/6J mice survived the infections with the different isolates. But in contrast to previous observations, not all A/J mice succumbed to infect...

journal_title：Experimental parasitology

authors： Hernandez-Valladares M,Naessens J,Nagda S,Musoke AJ,Rihet P,Ole-Moiyoi OK,Iraqi FA

更新日期：2004-11-01 00:00:00

abstract：:Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antig...

journal_title：Experimental parasitology

authors： Thiangtrongjit T,Adisakwattana P,Limpanont Y,Dekumyoy P,Nuamtanong S,Chusongsang P,Chusongsang Y,Reamtong O

更新日期：2018-08-01 00:00:00

abstract：:A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicop...

journal_title：Experimental parasitology

authors： Wu L,Chen SX,Jiang XG,Cao JP

更新日期：2009-12-01 00:00:00

abstract：:The main goal of the present study was to characterise the course of infection and immunological responses developed by Leishmania infantum infected BALB/c mice. Parasite load was determined by Real-time TaqMan PCR while cytokine and Immunoglobulin G (IgG) production were assessed by ELISA. Leishmania DNA was detected...

journal_title：Experimental parasitology

authors： Rolão N,Cortes S,Gomes-Pereira S,Campino L

更新日期：2007-03-01 00:00:00

abstract：:Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...

journal_title：Experimental parasitology

authors： Ramírez JD,Duque MC,Montilla M,Cucunubá Z,Guhl F

更新日期：2012-12-01 00:00:00

abstract：:Aedes albopictus mosquito is an opportunistic blood feeder and has a broad host range. The feeding behavior and habits of this mosquito are liable to increase the transmission potential of arboviruses. The survival and fecundity in A. albopictus fed on different hosts and post-blood meal provision of sugar were invest...

journal_title：Experimental parasitology

authors： Xue RD,Ali A,Barnard DR

更新日期：2008-06-01 00:00:00

abstract：:A limiting dilution assay (LDA) was developed for the quantification of Trypanosoma cruzi in the heart and blood of infected mice. Three groups of swiss mice were injected ip with "CL", "Colombiana," and "Y" strains. At 1-day intervals after infection, blood and the heart were removed. Serial blood dilutions in LIT me...

journal_title：Experimental parasitology

authors： Nunes MP,Coutinho SG,Louis JA,Souza WJ

更新日期：1990-02-01 00:00:00

abstract：:Amoebae of the genus Acanthamoeba are free-living protozoa that can cause granulomatous encephalitis and keratitis in humans. In this study, four clinical and three household dust isolates obtained in Vitória, Espírito Santo, Brazil were characterized by their morphological, genotypic, and physiological properties. Al...

journal_title：Experimental parasitology

authors： Duarte JL,Furst C,Klisiowicz DR,Klassen G,Costa AO

更新日期：2013-09-01 00:00:00

abstract：:Since we had previously demonstrated the protective role played by Toxoplasma excreted-secreted antigens, the aim of the present work was to produce monoclonal antibodies directed against these antigens in order to determine if their localization in the parasite is compatible with a mechanism of excretion or secretion...

journal_title：Experimental parasitology

authors： Charif H,Darcy F,Torpier G,Cesbron-Delauw MF,Capron A

更新日期：1990-07-01 00:00:00

abstract：:Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the s...

journal_title：Experimental parasitology

authors： Nayak A,Akpunarlieva S,Barrett M,Burchmore R

更新日期：2018-02-01 00:00:00

abstract：:Living Leishmania amazonensis amastigotes were incubated with radioiodinated N-benzyloxycarbonyl-L-tyrosyl-L-alanyl diazomethane (Z-Tyr-AlaCHN2), an irreversible inhibitor of mammalian cathepsins B and L. Parasite lysates were subjected to electrophoresis in gelatin-containing sodium dodecyl sulfate-acrylamide gels to...

journal_title：Experimental parasitology

authors： Alfieri SC,Pral EM,Shaw E,Ramazeilles C,Rabinovitch M

更新日期：1991-11-01 00:00:00

abstract：:Basic aspects of the generation, structure and function of Echinococcus granulosus protoscoleces are unknown. We review the work done on the structure and ultrastructure of the E. granulosus protoscolex and provide new data together with a comprehensive view of this form of the parasite. The surface, as observed by sc...

journal_title：Experimental parasitology

authors： Galindo M,Schadebrodt G,Galanti N

更新日期：2008-08-01 00:00:00

abstract：:The biochemical properties of the enzyme responsible for nematode "activated L-serine sulphydrase" activity (L-cysteine + R-SH----cysteine thioether + H2S) have been investigated using primarily the gastro-intestinal nematodes Nippostrongylus brasiliensis and Haemonchus contortus. The activated L-serine sulphydrase en...

journal_title：Experimental parasitology

authors： Walker J,Barrett J

更新日期：1992-03-01 00:00:00

abstract：:As an approach to better understand the molecular mechanisms underlying the differential cellular response to larval Schistosoma mansoni observed in Biomphalaria glabrata, we have analyzed hemocyte membrane-associated polypeptides from inbred susceptible and resistant strains of B. glabrata. A combination of technique...

journal_title：Experimental parasitology

authors： Coustau C,Yoshino TP

更新日期：1994-08-01 00:00:00

abstract：:Two experiments were performed to determine whether oral administration of copper oxide capsules controlled helminthic infections in Lacaune sheep without acute collateral effects on animal health. In experiment 1, 48 multiparous lactating sheep (60.1 ± 8.5 kg) were stratified according to initial number of eggs (Haem...

journal_title：Experimental parasitology

authors： Campigotto G,Gebert RR,Santos DS,Dos Reis JH,Alba DF,Cazarotto CJ,Leal MLR,Baldissera MD,Lopes TF,Druzian LT,Morsch VM,Vedovatto M,Da Silva AS

更新日期：2019-10-01 00:00:00