Abstract：
:The study of the encystation process of Entamoeba histolytica has been hampered by the lack of experimental means of inducing mature cysts in vitro. Previously we have found that cytoplasmic vesicles similar to the encystation vesicles of Entamoeba invadens are present in E. histolytica trophozoites only in amebas recovered from experimental amebic liver abscesses. Here we report that a monoclonal antibody (B4F2) that recognizes the cyst wall of E. invadens also identifies a 48 kDa protein in vesicles of E. histolytica trophozoites recovered from hepatic lesions. This protein is less expressed in trophozoites continuously cultured in axenical conditions. As previously reported for E. invadens, the B4F2 specific antigen was identified as enolase in liver-recovered E. histolytica, by two-dimensional electrophoresis, Western blot and mass spectrometry. In addition, the E. histolytica enolase mRNA was detected by RT PCR. The antigen was localized by immunoelectron microscopy in cytoplasmic vesicles of liver-recovered amebas. The B4F2 antibody also recognized the wall of mature E. histolytica cysts obtained from human samples. These results suggest that the enolase-containing vesicles are produced by E. histolytica amebas, when placed in the unfavorable liver environment that could be interpreted as an attempt to initiate the encystation process.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Segovia-Gamboa NC,Talamás-Rohana P,Ángel-Martínez A,Cázares-Raga FE,González-Robles A,Hernández-Ramírez VI,Martínez-Palomo A,Chávez-Munguía Bdoi
10.1016/j.exppara.2011.05.001subject
Has Abstractpub_date
2011-09-01 00:00:00pages
65-71issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(11)00150-0journal_volume
129pub_type
杂志文章abstract：:A comparative study of cell surface characteristics of pathogenic and nonpathogenic promastigotes of Leishmania braziliensis, NR and LBY strains, respectively, was carried out by means of concanavalin A agglutination and labeling with concanavalin A-fluorescein isothiocyanate, concanavalin A-ferritin, and cationized f...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(85)90071-2
更新日期：1985-04-01 00:00:00
abstract：:Optimization of desferrioxamine B (DFO) delivery for the treatment of malaria was studied in Plasmodium vinckei infected mice. DFO was administered by three different treatment regimens: (1) multiple subcutaneous injections of free DFO, (2) intraperitoneal infusion of free DFO, or (3) multiple subcutaneous injections ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4282
更新日期：1998-07-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.11.024
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2020.107918
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(87)90075-0
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(02)00016-4
更新日期：2002-03-01 00:00:00
abstract：:Chagas' disease is caused by unicellular parasite Trypanosoma cruzi (T. cruzi). It is endemic throughout Latin America, but nowadays has become a global challenge due to tourism and migration. Non-treated infection may result in health-threatening complications and lead to death. Current medications for this infection...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.12.013
更新日期：2017-01-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0047
更新日期：1996-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.06.010
更新日期：2016-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2019-02-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.08.009
更新日期：2016-11-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(92)90250-e
更新日期：1992-12-01 00:00:00
abstract：:Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C. sinensis. The primers designed from the nucleotide sequence data der...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2006.09.010
更新日期：2007-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0096
更新日期：1996-11-01 00:00:00
abstract：:Trypanosoma congolense is an important pathogen that wreaks havoc in the livestock industry of the African continent. This study evaluated the in vivo antitrypanosomal activity of geranylacetone and its ameliorative effect on the disease-induced anaemia and organ damages as well as its inhibitory effects against trypa...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2019.04.011
更新日期：2019-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.06.001
更新日期：2012-10-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2010.11.002
更新日期：2011-02-01 00:00:00
abstract：:Mitochondria from a rodent malarial parasite (Plasmodium berghei) were successfully purified by differential centrifugation and 22% Percoll density gradient separation. The purified mitochondria from the erythrocytic stages of the parasite had a density of 1.05 and were found to be heterogeneous by transmission electr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1993.1070
更新日期：1993-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2009-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.11.004
更新日期：2009-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(90)90099-x
更新日期：1990-02-01 00:00:00
abstract：:Acanthamoeba spp., known to cause keratitis and granulomatous encephalitis in humans, are frequently isolated from a variety of water sources. Here we report for the first time the characterization of an Acanthamoeba sp. (ACC01) isolated from tap water in Brazil. This organism is currently being maintained in an axeni...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.07.006
更新日期：2009-11-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1075
更新日期：1995-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.08.004
更新日期：2010-09-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1994.1030
更新日期：1994-05-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4362
更新日期：1999-02-01 00:00:00
abstract：:In Trichostrongylus colubriformis-infected rabbits, epithelial cell migration rates and cell transit times along the villi were compared by radioautography on histological slides to normal values from noninfected small intestine. Regions of gut with high (upper jejunum) and low (ileum) burdens of worms were both exami...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(89)90116-1
更新日期：1989-04-01 00:00:00
