Abstract:
:The intraerythrocytic malaria parasite digests haemoglobin to provide amino acids for metabolism and releases toxic haem that is sequestered into haemozoin, a non-toxic, insoluble, crystalline pigment. Following erythrocyte rupture, haemozoin is released into circulation and phagocytosed by monocytes. Phagocytosed haemozoin and antimalarial drugs have both been reported to modulate monocyte functions. This study determined the effects of therapeutic concentrations of seven antimalarial drugs; amodiaquine, artemisinin, chloroquine, doxycycline, primaquine, pyrimethamine and quinine, on the phagocytosis of β-haematin (synthetic haemozoin) by two monocytic cell lines, J774A.1 and U937, and human peripheral blood mononuclear cells. A novel spectrophotometric method based on the absorbance (O.D 400 nm) of alkali/SDS treated monocytes containing β-haematin was developed to complement counting phagocytosis with microscopy. The method has potential use for the large scale screening of monocyte phagocytic activity. Artemisinin, quinine, primaquine and pyrimethamine activated β-haematin phagocytosis by 12% or more, whereas amodiaquine, chloroquine and doxycyline inhibited β-haematin phagocytosis. In contrast, antimalarial drugs had minimal inhibitory effects on the phagocytosis of latex beads with only quinine resulting in more than 20% inhibition. Antimalarial drugs appear to alter monocyte phagocytic activity which has implications for the treatment, pathogenicity and adjunct therapies for malaria.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Cumming BM,Goldring JPDdoi
10.1016/j.exppara.2018.12.002subject
Has Abstractpub_date
2019-02-01 00:00:00pages
93-102eissn
0014-4894issn
1090-2449pii
S0014-4894(18)30119-Xjournal_volume
197pub_type
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doi:10.1016/j.exppara.2012.06.002
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2017.10.014
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journal_title:Experimental parasitology
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更新日期:2007-11-01 00:00:00
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journal_title:Experimental parasitology
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doi:10.1016/0014-4894(84)90023-7
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.11.007
更新日期:2006-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2019.02.003
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doi:10.1016/j.exppara.2012.05.005
更新日期:2012-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.12.007
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1045
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journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/0014-4894(91)90013-m
更新日期:1991-07-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.08.002
更新日期:2012-11-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.09.009
更新日期:2018-12-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1999.4448
更新日期:1999-10-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.06.014
更新日期:2016-11-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90036-6
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90116-1
更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章
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