Abstract:
:Clonorchis sinensis is a fish-borne trematode endemic to East Asia, which infects over 35 million people globally. In the study described here, we developed a nested polymerase chain reaction (PCR) method for the specific and reliable detection of C. sinensis. The primers designed from the nucleotide sequence data derived in this study were evaluated for their specificity and sensitivity for the detection of C. sinensis. The specific amplification products were obtained only with C. sinensis and no amplifications occurred with the DNA of closely related trematodes including Opisthorchis viverrini demonstrating the specificity of the assay. The novel PCR method described here will be useful for the quarantine of fishery products and evaluation of transmission status of clonorchiasis in the endemic areas.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Parvathi A,Sanath Kumar H,Kenchanna Prakasha B,Lu J,Xu X,Hu W,Feng Z,Karunasagar I,Karunasagar Idoi
10.1016/j.exppara.2006.09.010subject
Has Abstractpub_date
2007-03-01 00:00:00pages
291-5issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(06)00217-7journal_volume
115pub_type
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