Abstract:
:Mitochondria from a rodent malarial parasite (Plasmodium berghei) were successfully purified by differential centrifugation and 22% Percoll density gradient separation. The purified mitochondria from the erythrocytic stages of the parasite had a density of 1.05 and were found to be heterogeneous by transmission electron microscopy and rhodamine 123 fluorescence microscopy. Three marker enzymes, dihydroorotate dehydrogenase, cytochrome c reductase, and cytochrome c oxidase, were assessed during the organelle separation. Purification of cytochrome c oxidase was carried out from the purified mitochondria by using combination techniques of detergent solubilization and reduced cytochrome c-agarose affinity chromatography. The 560-fold purified enzyme with 3.6% yield was obtained and it had low catalytic efficiency with a kcat/Km of 5.9 x 10(-5) M-1 x min-1. The native form of the enzyme, determined by a gel filtration column on fast protein liquid chromatography, was found to be an oligomeric structure with a minimal molecular weight of 670 kDa. The malarial enzyme was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then compared to the enzyme obtained from host liver cells. These results suggested that the partially purified enzyme from the parasite was not different from its host mammalian cells. The importance of the enzyme in the erythrocytic phase of the parasite is discussed as a part of a simple electron transport system in mitochondrion linked to limited oxygen utilization and pyrimidine de novo biosynthesis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Krungkrai J,Krungkrai SR,Bhumiratana Adoi
10.1006/expr.1993.1070subject
Has Abstractpub_date
1993-09-01 00:00:00pages
136-46issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(83)71070-2journal_volume
77pub_type
杂志文章abstract::Schistosoma mansoni muscle fibers contract in response to L-glutamate in a dose-dependent manner (10(-6)-10(-3) M). L-aspartate and D-aspartate are likewise effective in eliciting contraction of the fibers. Mammalian glutamate receptor agonists produce little or no contraction at concentrations as high as 1 mM. In add...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0129
更新日期:1996-12-01 00:00:00
abstract::A murine model system was developed to study the induction and mechanism of protective immunity to L3 of Strongyloides stercoralis. L3 were implanted in BALB/cByJ mice in diffusion chambers constructed with 0.1- or 2.0-microns-pore-size membranes. Parasites survived equally well regardless or membrane type for 7 days,...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1036
更新日期:1995-03-01 00:00:00
abstract::This study aimed to investigate the effects of diphenyl diselenide (PhSe)2 to treat mice experimentally infected by Toxoplasma gondii on seric biomarkers of cardiac function (creatine kinase, creatine kinase MB, troponin, and myoglobin), and lactate dehydrogenase, as well as to evaluate the enzymatic activity of creat...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.04.014
更新日期:2016-08-01 00:00:00
abstract::We have studied inflammatory reactions in the mesenteric tissue of mice infected with Schistosoma mansoni. Perivascular tissue contained diffuse infiltrates of macrophages, eosinophilic granulocytes and lymphocytes. Angiogenesis in the perivascular adipose tissue was associated with superficial plasmacytogenic foci. P...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90203-m
更新日期:1992-06-01 00:00:00
abstract::Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.12.001
更新日期:2014-02-01 00:00:00
abstract::Only a few drugs are available for chemotherapy of African trypanosomiasis and there is an urgent need for the development of new anti-trypanosomal agents. In this study, the anti-helminthic drug niclosamide was tested for its trypanocidal activity in vitro using culture-adapted bloodstream forms of Trypanosoma brucei...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.12.001
更新日期:2008-04-01 00:00:00
abstract::In mice concurrently infected with Hymenolepis citelli and Nematospiroides dubius, survival of the tapeworm was prolonged, and there was an impairment of the efferent arm of the response to the cestode. The immunological rejection of a six cysticercoid primary H. citelli infection was delayed by the N. dubius infectio...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(85)90043-8
更新日期:1985-12-01 00:00:00
abstract::Early diagnosis and determining the infective stage are critical for effective therapy of toxoplasmosis. Owing to the progress in biotechnology, commonly used native, non-standardized diagnostic antigens should be replaced by genetically engineered antigens. The recombinant proteins are also promising components of su...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.01.006
更新日期:2015-03-01 00:00:00
abstract::Malaria caused by the protozoan parasite Plasmodium falciparum, is a major health problem of the developing world. In the present study medicinal plants from Eastern Ghats of South India have been extracted with ethyl acetate and assayed for growth inhibition of asexual erythrocytic stages of chloroquine (CQ)-sensitiv...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.01.021
更新日期:2013-05-01 00:00:00
abstract::A comparative study of cell surface characteristics of pathogenic and nonpathogenic promastigotes of Leishmania braziliensis, NR and LBY strains, respectively, was carried out by means of concanavalin A agglutination and labeling with concanavalin A-fluorescein isothiocyanate, concanavalin A-ferritin, and cationized f...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(85)90071-2
更新日期:1985-04-01 00:00:00
abstract::Factors which control the expression of the refractory or susceptible condition to infection with Dirofilaria immitis in the mosquito. Aedes aegypti, were investigated using three protocols. (1) Microfilariae and prelarvae were injected into the hemocoel of susceptible A. aegypti. Some microfilariae and prelarvae deve...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(88)90057-4
更新日期:1988-06-01 00:00:00
abstract::Schistosoma mansoni cercariae are stimulated by 2-tetradecenoic acid (TDA) to penetrate agar substrates. TDA simultaneously causes tegumental transformation similar to that seen when cercariae transform to schistosomula, reduces the Cercarienhüllen reaction in immune human serum, and reduces larval tolerance to water....
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(84)90037-7
更新日期:1984-10-01 00:00:00
abstract::Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantita...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.01.002
更新日期:2011-04-01 00:00:00
abstract::Infective larvae of Strongyloides spp. have been shown to contain azocollytic enzymes which may aid in host skin penetration. Attempts to demonstrate classical, neutral pH-active collagenase activity in Strongyloides ratti were unsuccessful. In the current study, we investigated the presence of acidic collagenolytic a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(87)90037-3
更新日期:1987-12-01 00:00:00
abstract::Murine malarial parasites have long been characterized by their requirement for either antibody-mediated immunity (AMI) or cell-mediated immunity (CMI) for suppression of acute parasitemia, with Plasmodium yoelii reportedly requiring AMI for suppression and P. chabaudi requiring CMI. To assess this characterization in...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.4132
更新日期:1997-02-01 00:00:00
abstract::Antimony unresponsiveness in mucocutaneous and visceral leishmaniasis is a serious clinical problem. Information on the mechanisms and characteristics of drug resistance in parasites that suggest chemotherapeutic strategies to overcome resistance is of practical importance. We developed nine lines of Leishmania resist...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90173-2
更新日期:1989-07-01 00:00:00
abstract::Cardiac beta-adrenergic receptors' function was studied in the acute phase of Chagas' disease in mice reinfected with Trypanosoma cruzi Tulahuen strain (Tul) and with parasites isolated from an infected patient (SGO-Z12). Genetic characterization of SGO-Z12 isolates demonstrated that it belongs to the zimodeme Z12, on...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(03)00096-1
更新日期:2003-03-01 00:00:00
abstract::Trophozoites and cysts of free-living Acanthamoeba castellanii present a serious risk to human health as causative agents of human diseases such as fatal granulomatous amoebic encephalitis and Acanthamoeba keratitis that is reported from various part of the world, also in Poland, with increasing frequency, particularl...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.01.025
更新日期:2010-09-01 00:00:00
abstract::Toxoplasma gondii is an intra-cellular parasite that infects humans through vertical and horizontal transmission. The cysts remain dormant in the brain of infected humans and can reactivate in immunocompromised hosts resulting in acute toxoplasmic encephalitis which may be fatal. We determined the onset and progressio...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.10.004
更新日期:2012-01-01 00:00:00
abstract::During its life cycle the malarial parasite Plasmodium forms three invasive stages which have to invade different and specific cells for replication to ensue. Invasion is vital to parasite survival and consequently proteins responsible for invasion are considered to be candidate vaccine/drug targets. Plasmodium perfor...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.01.015
更新日期:2007-08-01 00:00:00
abstract::Nine of 18 chimpanzees inoculated with 250 infective third-stage larvae (L3) each developed patent (i.e., positive for microfilariae) Onchocerca volvulus infection. Four of 6 infected chimpanzees that received 200 micrograms/kg ivermectin at 28 days postinfection (pi) became patent, whereas, when ivermectin was given ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90199-k
更新日期:1992-06-01 00:00:00
abstract::Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the para...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.03.020
更新日期:2013-06-01 00:00:00
abstract::The cDNA of a Schistosoma japonicum myophilin-like protein was cloned, sequenced, and expressed in Escherichia coli as a recombined protein (rSj myophilin-like protein), and the protein was purified by affinity chromatography. The deduced amino acid sequences of the Sj myophilin-like protein showed significant homolog...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.01.017
更新日期:2008-05-01 00:00:00
abstract::Amphotericin B (Amp) has been well-successfully used to treat against Leishmania infection, although high toxicity has been found in patients. In the present study, Amp was administered in Leishmania infantum-infected BALB/c mice by three distinct delivery systems aiming to compare their efficacy against challenge inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.02.003
更新日期:2018-03-01 00:00:00
abstract::We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens-Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)-and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.03.024
更新日期:2016-07-01 00:00:00
abstract::Nitric oxide (NO), derived from catalysis of inducible NO synthase (iNOS), limits malaria parasite growth in mammals. Transforming growth factor (TGF)-beta1 suppresses iNOS in cells in vitro as well as in vivo in mice, but paradoxically severe malaria in humans is associated with low levels of TGF-beta1. We hypothesiz...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.08.013
更新日期:2008-02-01 00:00:00
abstract::Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.04.016
更新日期:2015-07-01 00:00:00
abstract::Snails from a B. glabrata stock, selected for nonsusceptibility to the NMRI strain of S. mansoni, exhibited unusual schistosome infections, with multiple sporocysts prominent in the head-foot (foot-sporocysts). When F1 progeny from numerous crosses between well-defined susceptible (S) and resistant (R) parental snails...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1092
更新日期:1993-11-01 00:00:00
abstract::Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(90)90108-o
更新日期:1990-04-01 00:00:00
abstract::Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 f...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0047
更新日期:1996-06-01 00:00:00