Anti-Trypanosoma activity of bioactive metabolites from Photorhabdus luminescens and Xenorhabdus nematophila.

Abstract:

:Only two drugs are currently available for the treatment of Chagas disease and their effectiveness are unsatisfactory. Photorhabdus luminescens and Xenorhabdus nematophila, two enteric bacteria highly pathogenic to a broad range of insects, have been studied as potential source for bioactive metabolites against protozoa causing neglected tropical diseases. Therefore, we tested the in vitro anti-Trypanosoma cruzi activity of secreted metabolites from these bacteria. The conditioned medium of X. nematophila and P. luminescens showed significant parasiticidal activity in a concentration-dependent manner (IC50XN = 0.34 mg/mL, IC50PL = 1.0 mg/mL). The parasiticidal compound was identified as a small molecule stable to heating and pH changes ranging from 2 to 12. Moreover, anti-Trypanosoma molecules secreted by both bacteria stimulate the trypanocidal activity of macrophages by a mechanism independent of nitric oxide. Summarizing, our studies reveal that P. luminescens and X. nematophila are potential sources of putative novel drugs against Chagas disease.

journal_name

Exp Parasitol

authors

Antonello AM,Sartori T,Silva MB,Prophiro JS,Pinge-Filho P,Heermann R,da Silva OS,Romão PRT

doi

10.1016/j.exppara.2019.107724

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

107724

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(19)30016-5

journal_volume

204

pub_type

杂志文章
  • An experimental model of ameboma in guinea pig.

    abstract::Among the wide variety of clinicopathological manifestations of intestinal amebiasis, amebomas occur rarely and their pathogenesis is not well understood. When cholesterol-fed, 2- to 4-week-old guinea pigs were infected intracecally with a virulent, monoaxenic strain of Entamoeba histolytica, gross and histologically ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(92)90151-y

    authors: Bhatti HS,Bhushnurmath S,Mahajan RC,Ganguly NK,Sehgal R

    更新日期:1992-05-01 00:00:00

  • Morphological and quantitative aspects of nodule formation in hemolymph of the blowfly Chrysomya megacephala (Fabricius, 1794).

    abstract::Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The anal...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.09.009

    authors: Faraldo AC,Gregório EA,Lello E

    更新日期:2008-03-01 00:00:00

  • Trypanosoma cruzi: effects of repetitive stress during the development of experimental infection.

    abstract::Activation of the hypothalamus-pituitary-adrenal axis plays a major role in the suppression of the immune system. We have investigated the effects of repetitive stress on Wistar rats infected with the Y strain of Trypanosoma cruzi and a control group that underwent stressor stimuli by exposure to ether vapor for one m...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.03.009

    authors: Santos CD,Caldeira JC,Toldo MP,Prado JC

    更新日期:2005-06-01 00:00:00

  • Antigenic variation in Giardia lamblia.

    abstract::Giardia lamblia undergo surface antigenic variation in vitro and in vivo. The presence of variant trophozoites can be detected in clones after exposure to cytotoxic monoclonal antibodies. Surviving Giardia (progeny) no longer possess the initial major surface antigen which is replaced by new antigens. Exposure of a cl...

    journal_title:Experimental parasitology

    pub_type: 杂志文章,评审

    doi:10.1016/0014-4894(89)90104-5

    authors: Nash TE

    更新日期:1989-02-01 00:00:00

  • Antiprotozoal drug nitazoxanide enhances parasitemia, tissue lesions and mortality caused by Trypanosoma cruzi in murine model.

    abstract::Chagas' disease is caused by unicellular parasite Trypanosoma cruzi (T. cruzi). It is endemic throughout Latin America, but nowadays has become a global challenge due to tourism and migration. Non-treated infection may result in health-threatening complications and lead to death. Current medications for this infection...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.12.013

    authors: Valle-Reyes JS,Melnikov V,Dobrovinskaya O,Rodriguez-Hernández A,Wookee-Zea C,Pimientel-Rodrigez V,Rueda-Valdovinos G,Delgado-Enciso I,López-Lemus UA,Espinoza-Gómez F

    更新日期:2017-01-01 00:00:00

  • Plasmodium berghei: recombinant interferon-gamma and the development of parasitemia and cerebral lesions in malaria-infected mice.

    abstract::Mice infected with Plasmodium berghei K173-parasitized erythrocytes develop severe hypothermia followed by death as a consequence of murine cerebral malaria early in the second week after infection. A single intraperitoneal injection of 10(5) Units of IFN-gamma given between Day 4 and Day 6 postinfection results in a ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1993.1078

    authors: Curfs JH,van der Meide PH,Billiau A,Meuwissen JH,Eling WM

    更新日期:1993-09-01 00:00:00

  • Low levels of mammalian TGF-beta1 are protective against malaria parasite infection, a paradox clarified in the mosquito host.

    abstract::Nitric oxide (NO), derived from catalysis of inducible NO synthase (iNOS), limits malaria parasite growth in mammals. Transforming growth factor (TGF)-beta1 suppresses iNOS in cells in vitro as well as in vivo in mice, but paradoxically severe malaria in humans is associated with low levels of TGF-beta1. We hypothesiz...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.08.013

    authors: Luckhart S,Lieber MJ,Singh N,Zamora R,Vodovotz Y

    更新日期:2008-02-01 00:00:00

  • Involvement of the Leishmania donovani virulence factor A2 in protection against heat and oxidative stress.

    abstract::Leishmania is an obligate intracellular protozoan parasite that infects cells of the reticulo-endothelial system. Host defences against Leishmania include fever and oxidant production, and the parasite has developed a number of defence mechanisms to neutralize the host response. The Leishmania donovani A2 family of pr...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.06.001

    authors: McCall LI,Matlashewski G

    更新日期:2012-10-01 00:00:00

  • Antiproliferative and ultrastructural effects of BPQ-OH, a specific inhibitor of squalene synthase, on Leishmania amazonensis.

    abstract::Parasites of the Leishmania genus require for the growth and viability the de novo synthesis of specific sterols as such as episterol and 5-dehydroepisterol because cholesterol, which is abundant in their mammalian hosts, does not fulfill the parasite sterol requirements. Squalene synthase catalyzes the first committe...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.08.006

    authors: Rodrigues JC,Urbina JA,de Souza W

    更新日期:2005-12-01 00:00:00

  • Trypanosoma cruzi: the Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor.

    abstract::Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedd...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0036

    authors: Abuin G,Couto AS,de Lederkremer RM,Casal OL,Galli C,Colli W,Alves MJ

    更新日期:1996-04-01 00:00:00

  • Glycosidases in Leishmania and their importance for Leishmania in phlebotomine sandflies with special reference to purification and characterization of a sucrase.

    abstract::Culture forms of Leishmania (Leishmania) amazonensis (IFLA/BR/67/PH8) produce an extracellular enzyme that hydrolyzes sucrose molecules into their component monosaccharides. This is important because phlebotomine sand flies, the invertebrate hosts of Leishmania, ingest plant sap or aphid and coccid honeydew rich in su...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0055

    authors: Gontijo NF,Melo MN,Riani EB,Almeida-Silva S,Mares-Guia ML

    更新日期:1996-06-01 00:00:00

  • Trypanosoma cruzi: quantification in tissues of experimentally infected mice by limiting dilution analysis.

    abstract::A limiting dilution assay (LDA) was developed for the quantification of Trypanosoma cruzi in the heart and blood of infected mice. Three groups of swiss mice were injected ip with "CL", "Colombiana," and "Y" strains. At 1-day intervals after infection, blood and the heart were removed. Serial blood dilutions in LIT me...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(90)90099-x

    authors: Nunes MP,Coutinho SG,Louis JA,Souza WJ

    更新日期:1990-02-01 00:00:00

  • Giardia lamblia: the effects of extracts and fractions from Mentha x piperita Lin. (Lamiaceae) on trophozoites.

    abstract::Giardia lamblia is a parasite that causes giardiasis in humans and other mammals. The common treatment includes different classes of drugs, which were described to produce unpleasant side effects. Mentha x piperita, popularly known as peppermint, is a plant that is frequently used in the popular medicine to treat gast...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2006.05.001

    authors: Vidal F,Vidal JC,Gadelha AP,Lopes CS,Coelho MG,Monteiro-Leal LH

    更新日期:2007-01-01 00:00:00

  • Plasmodium coatneyi: differential clinical and immune responses of two populations of Macaca fascicularis from different origins.

    abstract::Two species of macaques, including two Macaca fascicularis from the Philippines, two M. fascicularis from Mauritius, and one Macaca mulatta, were experimentally infected with blood stages of Plasmodium coatneyi and followed during their clinical, parasitological, biological, and immunological evolution. Plasma cytokin...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1999.4342

    authors: Migot-Nabias F,Ollomo B,Dubreuil G,Morelli A,Domarle O,Nabias R,Georges AJ,Millet P

    更新日期:1999-01-01 00:00:00

  • Plasmodium falciparum: development and validation of a measure of intraerythrocytic growth using SYBR Green I in a flow cytometer.

    abstract::Reliable analytical techniques to test growth-promoting and antimalarial efficacy on plasmodia are very important. Flow cytometry (FCM) offers the possibility to study developmental stages of intraerythrocytic growth of malaria parasites using nucleic acid staining. To analyze the growth of Plasmodium falciparum SYBR ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.10.008

    authors: Izumiyama S,Omura M,Takasaki T,Ohmae H,Asahi H

    更新日期:2009-02-01 00:00:00

  • Plasmodium yoelii: contribution of oocysts melanization to natural refractoriness in Anopheles dirus.

    abstract::It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.02.013

    authors: Wen-Yue X,Jian Z,Tao-Li Z,Fu-Sheng H,Jian-Hua D,Ying W,Zhong-Wen Q,Li-Sha X

    更新日期:2007-08-01 00:00:00

  • Entamoeba histolytica: sequence conservation of the Gal/GalNAc lectin from clinical isolates.

    abstract::The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/s0014-4894(02)00113-3

    authors: Beck DL,Tanyuksel M,Mackey AJ,Haque R,Trapaidze N,Pearson WR,Loftus B,Petri WA

    更新日期:2002-06-01 00:00:00

  • Acanthamoeba: could it be an environmental host of Shigella?

    abstract::Shigellosis is a serious public health problem in Korea, because large outbreaks of Shigella sonnei infections were recorded in many parts of the country during the period 1998-2000. However, the epidemiological features of shigellosis are not well known. In this study, we devised conditions suitable for the growth an...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2006.08.002

    authors: Jeong HJ,Jang ES,Han BI,Lee KH,Ock MS,Kong HH,Chung DI,Seol SY,Cho DT,Yu HS

    更新日期:2007-02-01 00:00:00

  • Toxoplasma gondii aspartic protease 1 is not essential in tachyzoites.

    abstract::Aspartic proteases are important virulence factors for pathogens and are recognized as attractive drug targets. Seven aspartic proteases (ASPs) have been identified in Toxoplasma gondii genome. Bioinformatics and phylogenetic analyses regroup them into five monophyletic groups. Among them, TgASP1, a coccidian specific...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.05.003

    authors: Polonais V,Shea M,Soldati-Favre D

    更新日期:2011-08-01 00:00:00

  • Development of a molecular xenomonitoring protocol to assess filariasis transmission.

    abstract::According to the World Health Organization, lymphatic filariasis (LF), a mosquito-borne neglected tropical disease (NTD), should be eliminated as a public health concern by the end of 2020. To this end, the goals of the Global Programme to Eliminate Lymphatic Filariasis (GPELF) include interrupting transmission throug...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107918

    authors: Albuquerque AL,Araújo TA,Melo DCTV,Paiva MHS,Melo FL,Oliveira CMF,Ayres CFJ

    更新日期:2020-08-01 00:00:00

  • Brachymeria lasus: effects of nutrient level on in vitro larval growth of a chalcid insect parasite.

    abstract::Exponential weight gain equations and growth rates were determined during larval development of Brachymeria lasus reared aseptically on chemically defined media with varying amino acid and glucose levels. The rate of weight gain increased from 0 to 2% dietary glucose and mean growth rate increased from 160 to 260 micr...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(83)90028-0

    authors: Thompson SN

    更新日期:1983-06-01 00:00:00

  • Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni.

    abstract::Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the para...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.03.020

    authors: Faro MJ,Perazzini M,Corrêa Ldos R,Mello-Silva CC,Pinheiro J,Mota EM,de Souza S,de Andrade Z,Júnior AM

    更新日期:2013-06-01 00:00:00

  • Susceptibility of clinical isolates of Leishmania aethiopica to miltefosine, paromomycin, amphotericin B and sodium stibogluconate using amastigote-macrophage in vitro model.

    abstract::Cutaneous Leishmaniasis (CL) caused by Leishmania aethiopica is a public health and social problem with a sequel of severe and mutilating skin lesions. It is manifested in three forms: localized CL (LCL), mucosal CL (MCL) and diffuse CL (DCL). Unresponsiveness to sodium stibogluconate (Sb(V)) is common in Ethiopian CL...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.01.022

    authors: Utaile M,Kassahun A,Abebe T,Hailu A

    更新日期:2013-05-01 00:00:00

  • Schistosoma mansoni: influence of infection on mouse behavior.

    abstract::Schistosoma mansoni infection in humans and animals induces abnormal neurobehavioral responses following granuloma formation. In mice, granulomas in the liver are observed 8 weeks after infection, while after 15-20 weeks, the presence of eggs and granulomas in the brain has been reported. In this study, outbred CD-1 f...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0047

    authors: Fiore M,Moroni R,Alleva E,Aloe L

    更新日期:1996-06-01 00:00:00

  • Light and scanning electron microscopy study of in vitro effects of artesunate in newly excysted metacercariae of Echinostoma paraensei (Trematoda: Digenea).

    abstract::Chemotherapy of food-borne trematodes relies on two drugs, praziquantel and tricabendazole, and there is growing interest in finding alternative therapies. Plant oil extracts have long been used in traditional Chinese medicine as sources of bioactive compounds with antiparasitic activity. Species of the genus Echinost...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2017.01.003

    authors: Souza JG,Lopes Torres EJ,Garcia JS,Gomes AP,Rodrigues-Silva R,Maldonado A Jr,Machado-Silva JR

    更新日期:2017-03-01 00:00:00

  • A new species and record of branchial parasitic isopods (Crustacea: Isopoda: Bopyridae: Pseudioninae) of porcellanid crabs from the Philippines.

    abstract::Branchial bopyrids infesting porcellanid crabs from the Philippines were investigated based on intertidal collections made in 1999-2000. Crabs of the genus Petrolisthes collected from sites in the northern Philippines were examined and two parasite species were found. One new pseudionine species found infesting Petrol...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.09.012

    authors: Williams JD,Madad AZ

    更新日期:2010-05-01 00:00:00

  • Behavioral changes in host foraging: Experiments with Clinostomum (Trematoda, Digenea) parasitizing Loricariichthys platymetopon (Loricariidae).

    abstract::Complex life cycle parasites can manipulate the behavior of intermediate hosts in order to reduce their fitness and increase the chance of completing life cycle. In order to understand the effects of the trematode parasites of the genus Clinostomum on host fish Loricariichthys platymetopon, a filmed experiment was car...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107916

    authors: Nicola DN,Affonso IP,Takemoto RM

    更新日期:2020-09-01 00:00:00

  • Grillotia erinaceus (Cestoda, Trypanorhyncha): localization of neuroactive substances in the plerocercoid, using confocal and electron-microscopic immunocytochemistry.

    abstract::Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1103

    authors: Halton DW,Maule AG,Brennan GP,Shaw C,Stoitsova SR,Johnston CF

    更新日期:1994-11-01 00:00:00

  • Schistosoma mansoni: protein phosphorylation during transformation of cercariae to schistosomula.

    abstract::Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90024-q

    authors: Wiest PM,Olds GR,Bowen WD

    更新日期:1991-08-01 00:00:00

  • Expression profile and subcellular localization of HslV, the proteasome related protease from Trypanosoma cruzi.

    abstract::Trypanosoma cruzi is a rare example of an eukaryote that has genes for two threonine proteases: HslVU complex and 20S proteasome. HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase. In this study, we expressed and obtained specific antibodies to HslU and ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.10.011

    authors: Barboza NR,Cardoso J,de Paula Lima CV,Soares MJ,Gradia DF,Hangai NS,Bahia MT,de Lana M,Krieger MA,Guerra de Sá R

    更新日期:2012-02-01 00:00:00