Abstract：
:Triosephosphate isomerase (TIM) is a major enzyme in the glycolytic pathway, which catalyzes the interconversion of glyceraldehyde 3-phosphate to dihydroxyacetone phosphate. Here, we report cloning, expression and purification of a catalytically active recombinant TIM of Leishmania donovani (LdTIM). The recombinant LdTIM had a pH optimum in the range of 7.2-9.0, found stable at 25°C for 30 min and K(m) and V(max) for the substrate glyceraldehyde 3-phosphate was 0.328±0.02mM and 10.05mM/min/mg, respectively. The cysteine-reactive agent methylmethane thiosulphonate (MMTS) was used as probe, in order to test its effect on enzyme activity. The MMTS induced 75% enzyme inactivation within 15 min at 250 μM concentration. The biochemical characterization of LdTIM described in this work is the essential step towards deeper understanding of its role in parasite survival. The purification of LdTIM in bioactive form provides important tools for further functional and structural studies.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Kumar K,Bhargava P,Roy Udoi
10.1016/j.exppara.2012.01.016subject
Has Abstractpub_date
2012-04-01 00:00:00pages
430-6issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(12)00033-1journal_volume
130pub_type
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