Abstract：
:Spent mushroom compost (SMC) is a residue generated in edible mushrooms production, such as Hypsizygus marmoreus. Its genome was recently sequenced, demonstrating cuticle-degrading protease genes. The present work aims to investigate the proteases from H. marmoreus spent mushroom compost (SMC) by verifying its action on nematode larvae. The extraction of the crude extract directly with water from H. marmoreus SMC proved to be efficient for proteases obtainment, with proteolytic activity of 195.36 ± 18.38 U g-1 of compound. Moreover, the zymogram and SDS-PAGE indicated the presence of two proteases with estimated molecular weights of 30.2 and 33.7 kDa. Due to the protease activity present in H. marmoreus SMC extract, there was a significant reduction in the number of Panagrellus redivivus and L3 in treated group compared to control group (p < 0.01), with 52% and 26% of reduction, respectively. A0A151VWY3 mature protein is composed of 296 amino acid residues, exhibiting molecular weight and pI of 29.5 kDa and 6.72. A0A151WD28 mature protein is composed of 343 amino acid residues, exhibiting molecular weight and pI of 34.4 kDa and 8.04. In the present work it was demonstrated that SMC from H. marmoreus has easily extracted protease content, presenting two proteases, possibly with cuticle-degrading activity, which had significant nematicidal effect on P. redivivus and bovine infective larvae.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Soares FEF,Nakajima VM,Sufiate BL,Satiro LAS,Gomes EH,Fróes FV,Sena FP,Braga FR,de Queiroz JHdoi
10.1016/j.exppara.2018.12.006subject
Has Abstractpub_date
2019-02-01 00:00:00pages
16-19eissn
0014-4894issn
1090-2449pii
S0014-4894(18)30083-3journal_volume
197pub_type
杂志文章abstract：:Transforming growth factor-β (TGF-β) signaling pathway is documented to participate in liver fibrosis via multifactorial mechanisms. microRNA Let-7b (Let-7b) has been proved to alleviate cell fibrosis through regulating TGF-β receptor I (TβRI), but whether it is involved in Schistosomiasis liver fibrosis (SLF) has not...
journal_title：Experimental parasitology
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abstract：:Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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abstract：:Trypanosoma cruzi is a rare example of an eukaryote that has genes for two threonine proteases: HslVU complex and 20S proteasome. HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase. In this study, we expressed and obtained specific antibodies to HslU and ...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2017.02.002
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(83)90008-5
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abstract：:The infection with blood stages of Plasmodium chabaudi chabaudi (AS) was followed in BALB/c and DBA/2 mice. Both strains show a peak parasitemia by 7-9 days after infection, display splenic hypercellularity of T and B cells, thymic atrophy, nearly complete depletion of B cells in the bone marrow, and mount comparable ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.03.024
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.09.014
更新日期：2013-11-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0119
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.09.012
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(85)90039-6
更新日期：1985-12-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.04.005
更新日期：2014-07-01 00:00:00
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2005.03.009
更新日期：2005-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exppara.2007.02.013
更新日期：2007-08-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1996.0036
更新日期：1996-04-01 00:00:00
abstract：:Protein tyrosine phosphorylation (PY) is a well-known signalling mechanism which is also involved in host-parasite interactions. Despite its transcendence, PY has been poorly studied in parasitic helminths. The aim of this study is to examine the effect of bile salts on the PY pattern in parasitic trematodes. Two dist...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.01.006
更新日期：2004-01-01 00:00:00
abstract：:Trypanosoma congolense is an important pathogen that wreaks havoc in the livestock industry of the African continent. This study evaluated the in vivo antitrypanosomal activity of geranylacetone and its ameliorative effect on the disease-induced anaemia and organ damages as well as its inhibitory effects against trypa...
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2005.07.006
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pub_type： 杂志文章
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doi：10.1016/j.exppara.2012.06.002
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journal_title：Experimental parasitology
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doi：10.1016/j.exppara.2012.06.009
更新日期：2012-10-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2004.03.012
更新日期：2004-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2007.01.015
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.01.016
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.11.007
更新日期：2006-04-01 00:00:00
abstract：:The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(02)00113-3
更新日期：2002-06-01 00:00:00
abstract：:Cytoadherence of Plasmodium falciparum-infected erythrocytes to amelanotic melanoma cells was pH dependent; increased adherence was observed in the pH range of 6.1 to 6.8 and was greatest between pH 6.6 and 6.8 Ca2+ promoted cytoadherence, but at higher concentrations (40-50 mM) than is usually the case for cell-cell ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(91)90108-9
更新日期：1991-10-01 00:00:00
