Trypanosomatidae: a spliced-leader-derived probe specific for the genus Phytomonas.

Abstract:

:We probed DNA from all trypanosomatid genera by slot blot hybridization with an oligonucleotide (SL3') complementary to a sequence of the Phytomonas spliced-leader or mini-exon RNA. The 19-nucleotide probe target site was previously shown to be highly conserved among a limited number of Phytomonas isolates, but diverges in other kinetoplastid genera. Our examination of 84 isolates of various genera of trypanosomatids showed hybridization of this probe exclusively with isolates from plants or insects which could, by morphological, biochemical, and molecular criteria, be considered to belong to the genus Phytomonas. In contrast, no hybridization was observed with flagellates of the genera Blastocrithidia, Crithidia, Endotrypanum, Herpetomonas, Leptomonas, Leishmania, and Trypanosoma. The method detected DNA quantities as low as 50 ng using either radioactive or nonradioactive probes, and was effective with as few as 10(4) intact flagellates. Together, these results suggest that this probe will serve as a convenient marker for taxonomic and epidemiological studies requiring reliable identification of Phytomonas spp. in plants or in putative insect vectors.

journal_name

Exp Parasitol

authors

Teixeira MM,Serrano MG,Nunes LR,Campaner M,Buck GA,Camargo EP

doi

10.1006/expr.1996.0119

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

311-9

issue

3

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(96)90119-8

journal_volume

84

pub_type

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