Abstract:
:Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Azzouz S,Maache M,Osuna A,Lawton P,Pétavy AFdoi
10.1016/j.exppara.2011.09.012subject
Has Abstractpub_date
2012-01-01 00:00:00pages
63-8issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(11)00284-0journal_volume
130pub_type
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