Abstract:
:Transforming growth factor-β (TGF-β) signaling pathway is documented to participate in liver fibrosis via multifactorial mechanisms. microRNA Let-7b (Let-7b) has been proved to alleviate cell fibrosis through regulating TGF-β receptor I (TβRI), but whether it is involved in Schistosomiasis liver fibrosis (SLF) has not been determined. In the present, SLF mice model was used to investigate Let-7b's function and mechanism in SLF. We found that hepatic let-7b expression was continuously declined in SLF, accompanied by the induction of TGF-β pathway molecules (TGF-β1, TβRI), profibrogenic mediators (α-SMA, colla I), and Th1/Th2 cells response factors (IFN-γ, IL-4). When recombinant Lentivirus of let-7b (Lenti-let-7b) was transfected into S. japonicum-infected mice, the mice hepatic fibrosis was distinctly ameliorated, and TGF-β1, TβRI, α-SMA, and colla I expressions were remarkly decreased, mice serum IL-4 and IFN-γ levels were reduced. Similarly, over-expression of let-7b down-regulated the expression of TβRI in THP-1 cells transfected with let-7b mimics, while TβRI was up-regulated after treated with let-7b inhibitor. These findings suggested that let-7b is a negative regulator to SLF through downregulating TβRI, and inhibits Th1 and Th2 type cell immune response. This provides a novel potential therapeutic strategy for SFL prevention.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Tang N,Wu Y,Cao W,Liang Y,Gao Y,Hu L,Yang Q,Zhou Y,Tang F,Xiao Jdoi
10.1016/j.exppara.2017.09.024subject
Has Abstractpub_date
2017-11-01 00:00:00pages
45-53eissn
0014-4894issn
1090-2449pii
S0014-4894(17)30200-Xjournal_volume
182pub_type
杂志文章abstract::The sequential changes in the histology in scabietic lesions were investigated for hosts (rabbits) during sensitization and reinfestation with Sarcoptes scabiei. Experimentally infested naive hosts developed heavy scabies infestations but 65% of the hosts showed induced immunity (resistance) to reinfestation. All host...
journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0036
更新日期:1996-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-06-01 00:00:00