A sensitive method for assaying chemotaxic responses of Leishmania promastigotes.

Abstract:

:This article describes a sensitive, cheap, and easy method for assaying chemotaxic responses of Leishmania promastigotes. A gradient of the substance to be assayed was produced inside a series of commercially available capillary tubes submerged in a promastigote suspension. After an incubation period, the attractiveness of the substance under test was measured by counting the number of parasites in the capillaries in a Neubauer chamber. Different responses were detected in two strains of Leishmania amazonensis and one strain of L. chagasi after standardization of the method to assay attraction to carbohydrates. Very different responses were obtained when the test was performed using promastigotes of the same strain in two different physiological states (log and stationary phase). The stationary phase cells showed an enhanced chemotaxic capability, which can be explained by the fact that the metacyclic forms commonest in stationary phase cultures have greater mobility than other promastigotes. This method will permit studies to be made of the attractive response to different substances in Leishmania species and other trypanosomatids and facilitate characterization of the potential receptors involved in the chemotaxic response. An adaptation of the method to assay the response to repellent substances is also provided.

journal_name

Exp Parasitol

authors

Oliveira JS,Melo MN,Gontijo NF

doi

10.1006/expr.2000.4569

keywords:

subject

Has Abstract

pub_date

2000-11-01 00:00:00

pages

187-9

issue

3

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(00)94569-7

journal_volume

96

pub_type

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