Abstract:
:Molecular characterizations of Cryptosporidium spp. in dairy cattle in industrialized nations have mostly shown a dominance of Cryptosporidium parvum, especially its IIa subtypes in pre-weaned calves. Few studies, however, have been conducted on the distribution of Cryptosporidium species and C. parvum subtypes in various age groups of dairy cattle in developing countries. In this study, we examined the prevalence and molecular characteristics of Cryptosporidium in dairy cattle in four Nile River delta provinces in Egypt. Modified Ziehl-Neelsen acid-fast microscopy was used to screen for Cryptosporidium oocysts in 1974 fecal specimens from animals of different ages on 12 farms. Positive fecal specimens were identified from all studied farms with an overall prevalence of 13.6%. By age group, the infection rates were 12.5% in pre-weaned calves, 10.4% in post-weaned calves, 22.1% in heifers, and 10.7% in adults. PCR-RFLP and DNA sequence analyses of microscopy-positive fecal specimens revealed the presence of four major Cryptosporidium species. In pre-weaned calves, C. parvum was most common (30/69 or 43.5%), but Cryptosporidium ryanae (13/69 or 18.8%), Cryptosporidium bovis (7/69 or 10.2%), and Cryptosporidium andersoni (7/69 or 10.2%) were also present at much higher frequencies seen in most industrialized nations. Mixed infections were seen in 12/69 (17.4%) of genotyped specimens. In contrast, C. andersoni was the dominant species (193/195 or 99.0%) in post-weaned calves and older animals. Subtyping of C. parvum based on sequence analysis of the 60kDa glycoprotein gene showed the presence of subtypes IIdA20G1 in nine specimens, IIaA15G1R1 in 27 specimens, and a rare subtype IIaA14G1R1r1b in one specimen. The common occurrence of non-C. parvum species and IId subtypes in pre-weaned calves is a distinct feature of cryptosporidiosis transmission in dairy cattle in Egypt. The finding of the same two dominant IIa and IId C. parvum subtypes recently found in humans in Egypt suggests calves can be potential reservoirs of zoonotic cryptosporidiosis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Amer S,Zidan S,Adamu H,Ye J,Roellig D,Xiao L,Feng Ydoi
10.1016/j.exppara.2013.09.002subject
Has Abstractpub_date
2013-11-01 00:00:00pages
518-23issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(13)00240-3journal_volume
135pub_type
杂志文章abstract::This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.03.007
更新日期:2007-09-01 00:00:00
abstract::A new method for measuring the size of parasites and other objects using optical microscopy was developed using a specifically designed movable computer ruler (MCR) derived from digital images of a stage micrometer. Subsequently, MCR can be superimposed on images of parasites to measure their size. MCR derived from th...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.10.008
更新日期:2012-01-01 00:00:00
abstract::Hemocytes derived from a strain (13-16-R1) of Biomphalaria glabrata resistant to Schistosoma mansoni were significantly more likely to bind untreated latex beads than hemocytes from the schistosome-susceptible M line strain. Beads preincubated in 13-16-R1 plasma were more readily bound by both 13-16-R1 and M line hemo...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90182-a
更新日期:1992-09-01 00:00:00
abstract::The concentration of L-hydroxyproline in the liver of ICR female mice increased rapidly during the 8th to 11th weeks of Schistosoma mansoni infection. Free L-proline concentration began to increase about the 7th week and reached its maximum at the 8th to 9th weeks of the infection, when the granulomatous response to t...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90131-f
更新日期:1991-02-01 00:00:00
abstract::Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.06.010
更新日期:2016-09-01 00:00:00
abstract::Feeding behavior and some antihemostatic properties of the saliva of Rhodnius prolixus infected with Trypanosoma rangeli were studied and compared with those of uninfected controls. Insects with salivary gland infection pierced host skin more often and drew less blood and at a lower rate than controls when feeding on ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1030
更新日期:1994-05-01 00:00:00
abstract::The intraerythrocytic malaria parasite digests haemoglobin to provide amino acids for metabolism and releases toxic haem that is sequestered into haemozoin, a non-toxic, insoluble, crystalline pigment. Following erythrocyte rupture, haemozoin is released into circulation and phagocytosed by monocytes. Phagocytosed hae...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.12.002
更新日期:2019-02-01 00:00:00
abstract::Chromosome-sized DNA molecules from Trypanosoma cruzi clone Dm 28c were analyzed and compared with other T. cruzi strains and monogenetic trypanosomatids by orthogonal field alteration gel electrophoresis. The results showed that T. cruzi Dm 28c displays at least 18 chromosomes ranging from 550 to more than 1500 kb an...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90178-1
更新日期:1989-08-01 00:00:00
abstract::Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.03.026
更新日期:2005-08-01 00:00:00
abstract::Twenty-one Trypanosoma cruzi stocks isolated from Triatoma infestans and humans of the Chilean Andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. A phenotypical characterization was performed by prote...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1100
更新日期:1995-08-01 00:00:00
abstract::Under a variety of stress conditions, Leishmania species display some morphological and biochemical features characteristic of mammalian programmed cell death or necrosis. Nitroheteroaryl-1,3,4-thiadiazoles induce cell death in Leishmania major (L. major). Putative mechanisms of action of these compounds were investig...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.06.002
更新日期:2012-10-01 00:00:00
abstract::Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Es...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2017.10.011
更新日期:2017-12-01 00:00:00
abstract::Traditional classification of Demodex mites by hosts and phenotypic characteristics is defective because of environmental influences. In this study, we proposed molecular identification of four phenotypes of two human Demodex species based on mitochondrial cox1 fragments for the first time. Mites collected from suffer...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.04.003
更新日期:2014-07-01 00:00:00
abstract::Transforming growth factor-β (TGF-β) signaling pathway is documented to participate in liver fibrosis via multifactorial mechanisms. microRNA Let-7b (Let-7b) has been proved to alleviate cell fibrosis through regulating TGF-β receptor I (TβRI), but whether it is involved in Schistosomiasis liver fibrosis (SLF) has not...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2017.09.024
更新日期:2017-11-01 00:00:00
abstract::Strongyloidiasis is a parasitosis that represents a public health problem, in tropical regions. The present study aimed to investigate the anthelmintic effects of several extracts of Argemone mexicana, as well as its main component berberine (Ber) against the third-stage larvae (L3) of Strongyloides venezuelensis in-v...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2020.108043
更新日期:2021-01-01 00:00:00
abstract::Amoebae of the genus Acanthamoeba are free-living protozoa that can cause granulomatous encephalitis and keratitis in humans. In this study, four clinical and three household dust isolates obtained in Vitória, Espírito Santo, Brazil were characterized by their morphological, genotypic, and physiological properties. Al...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.05.013
更新日期:2013-09-01 00:00:00
abstract::Benznidazole is the first-line drug used in treating Chagas disease, which is caused by the parasite Trypanosoma cruzi (T. cruzi). However, benznidazole has limited efficacy and several adverse reactions. Pentamidine is an antiprotozoal drug used in the treatment of leishmaniasis and African trypanosomiasis. In T. cru...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.10.007
更新日期:2016-12-01 00:00:00
abstract::The compound 2-hydroxy-3-(1'-propen-3-phenyl)-1,4-naphthoquinone (PHNQ6) was evaluated for activity against Toxoplasma gondii, alone or combined with sulfadiazine. Treatment with PHNQ6 combined with sulfadiazine protected at least 70 and 90% of mice infected with RH and EGS strains, respectively. Mice were treated wit...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.12.006
更新日期:2006-06-01 00:00:00
abstract::The blood dwelling stages of schistosomes have acetylcholinesterase (AChE) and nicotinic-like acetylcholine receptors (nAChR) on their teguments. Both AChE and nAChR are concentrated on the dorsal surface of the adult male, a major surface for nutrient uptake for the worm pair. Exposure of tegumental AChE and nAChR to...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1152
更新日期:1995-12-01 00:00:00
abstract::A monoclonal antibody was generated against the large subunit of Schistosoma japonicum calpain to study the localization and possible function of the molecule in vivo. Mice were immunized with recombinant S. japonicum calpain and polyclonal antisera and a monoclonal antibody specific to schistosome calpain was obtaine...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2004.11.001
更新日期:2005-01-01 00:00:00
abstract::In the search of new alternatives for neurocysticercosis treatment, the cysticidal activity of organic extracts of Teloxys graveolens was evaluated. The in vitro activity of hexane, ethyl acetate and methanol extracts against Taenia crassiceps cysts was tested and the selectivity index relative to human fibroblasts wa...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.06.001
更新日期:2015-09-01 00:00:00
abstract::The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2001.4622
更新日期:2001-06-01 00:00:00
abstract::In March 2010, a 35 year-old HIV/AIDS female patient was admitted to hospital to start treatment with Highly Active Antiretroviral Therapy (HAART) since during a routine control a dramatic decrease in the CD4(+) levels was detected. At this stage, a nasal swab from each nostril was collected from the patient to includ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.02.014
更新日期:2014-11-01 00:00:00
abstract::Snails from a B. glabrata stock, selected for nonsusceptibility to the NMRI strain of S. mansoni, exhibited unusual schistosome infections, with multiple sporocysts prominent in the head-foot (foot-sporocysts). When F1 progeny from numerous crosses between well-defined susceptible (S) and resistant (R) parental snails...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1993.1092
更新日期:1993-11-01 00:00:00
abstract::Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as ass...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.09.012
更新日期:2012-01-01 00:00:00
abstract::Previously generated two auxotrophic genes knockout Edwardsiella tarda (Δalr Δasd E. tarda) was used as a delivery vehicle for CpG 1668 motif-enriched plasmids (pL-CpG30), and potential of the Δalr Δasd E. tarda-mediated delivery of CpG motifs in both prevention and therapeutic treatment of scuticociliatosis caused by...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.06.007
更新日期:2014-09-01 00:00:00
abstract::Environmental stress and endocrine control can affect pathogenesis of sexually transmitted diseases such as trichomoniasis. Acute Tritrichomonas foetus infection was compared in female BALB/c mice to infections in mice treated with high doses of estradiol or housed in constant bright illumination (stressed). In untrea...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.07.009
更新日期:2007-02-01 00:00:00
abstract::The Gal/GalNAc lectin gene of Entamoeba histolytica is a major amebic virulence protein responsible for interaction with host tissues. We investigated sequence differences in the Gal/GalNAc lectin heavy subunit in three isolates from Bangladesh and one isolate from Georgia, each of which was determined to be genetical...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(02)00113-3
更新日期:2002-06-01 00:00:00
abstract::Entamoeba histolytica programmed cell death (PCD) induced by G418 is characterized by the release of important amounts of intracellular calcium from reservoirs. Nevertheless, no typical caspases have been detected in the parasite, the PCD phenotype is inhibited by the cysteine protease inhibitor E-64. These results st...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2015.10.005
更新日期:2015-12-01 00:00:00
abstract::Six chimpanzees (Pan troglodytes) and six mangabey monkeys (Cercocebus atys) were inoculated with Onchocerca volvulus third-stage larvae (L3) of West African origin. Two chimpanzees each received 200, 300, or 400 L3, while three mangabeys each received either 50 or 250 L3. All six chimpanzees became microfilaria posit...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1057
更新日期:1995-05-01 00:00:00