Abstract:
:The main goal of the present study was to characterise the course of infection and immunological responses developed by Leishmania infantum infected BALB/c mice. Parasite load was determined by Real-time TaqMan PCR while cytokine and Immunoglobulin G (IgG) production were assessed by ELISA. Leishmania DNA was detected in spleen and liver as soon as day 1 post-inoculation (pi) and the parasitism was sustained until the end of the experiment. The cytokine kinetics in spleen and liver was generally associated with the oscillations of parasite load. Overall, it was not observed a distinct Th1 or Th2 pattern of cytokine production during the time of experiment. The infected mice developed a mixed immune response, with concomitant production of IFN-gamma, IL-4 and IL-10, both in spleen and liver, and both IgG isotypes. However, our results suggest that, compared to liver, the spleen is more susceptible to L. infantum infection.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Rolão N,Cortes S,Gomes-Pereira S,Campino Ldoi
10.1016/j.exppara.2006.09.013subject
Has Abstractpub_date
2007-03-01 00:00:00pages
270-6issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(06)00220-7journal_volume
115pub_type
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1091
更新日期:1994-11-01 00:00:00
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abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
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