Entamoeba histolytica: identification of EhGPCR-1, a novel putative G protein-coupled receptor that binds to EhRabB.

Abstract:

:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment that encodes the C-terminus of EhGPCR-1. The cloning and sequence of the full-length cDNA revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocytosis and seven transmembranal domains. These results suggest that EhGPCR-1 could be a GPCR involved in phagocytosis. EhGPCR-1 could be a member of the Rhodopsin family, characterized by a short N-terminus without cysteine residues.

journal_name

Exp Parasitol

authors

Picazarri K,Luna-Arias JP,Carrillo E,Orozco E,Rodriguez MA

doi

10.1016/j.exppara.2005.02.014

keywords:

subject

Has Abstract

pub_date

2005-07-01 00:00:00

pages

253-8

issue

3

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(05)00065-2

journal_volume

110

pub_type

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