Abstract:
:EhRabB is an Entamoeba histolytica protein involved in phagocytosis. However, proteins that regulate the EhRabB activity are unknown. Here, we report the identification of a putative G protein-coupled receptor of E. histolytica (EhGPCR-1) that binds to EhRabB. By two-hybrid screening, we found a 372-bp cDNA fragment that encodes the C-terminus of EhGPCR-1. The cloning and sequence of the full-length cDNA revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocytosis and seven transmembranal domains. These results suggest that EhGPCR-1 could be a GPCR involved in phagocytosis. EhGPCR-1 could be a member of the Rhodopsin family, characterized by a short N-terminus without cysteine residues.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Picazarri K,Luna-Arias JP,Carrillo E,Orozco E,Rodriguez MAdoi
10.1016/j.exppara.2005.02.014keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
253-8issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(05)00065-2journal_volume
110pub_type
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