Abstract:
:While in medium containing glucose, schistosomes exhibit homolactic fermentation. Accumulation of lactate acid in tissue fluid causes lowering of pH and a resultant inhibition of metabolic pathways. This requires lactate transporter protein in homolactic fermentors to facilitate the translocation of lactate(-) and [H(+)] across their plasma membrane. The ex-vivo experiment assessed lactic acid secretion by adult worms in absence and the presence of lactic acid transporter protein inhibitors. Phloretin and alpha-cyano-4-hydroxycinnamate caused a combined 25-35% inhibition of lactic acid secretion and probenecid increased this inhibition to 65% of control values. The removal of inhibitors resulted in 80% recovery of lactic acid secretion. In the in-vitro studies using vesicles isolated from adult worms and from schistosomula, the effects of phloretin and alpha-cyano-4-hydroxycinnamate were greater, each causing approximately 80% inhibition independently. The data obtained in this study demonstrate the presence of lactic acid transporters or similar proteins in Schistosoma mansoni.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Githui EK,Damian RT,Aman RAdoi
10.1016/j.exppara.2006.03.007subject
Has Abstractpub_date
2006-11-01 00:00:00pages
180-8issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(06)00069-5journal_volume
114pub_type
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journal_title:Experimental parasitology
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doi:10.1016/j.exppara.2009.02.009
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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