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Acanthamoeba spp.: in vitro effects of clinical isolates on murine macrophages, osteosarcoma and HeLa cells.

Abstract：

:Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were carried out with these strains and the type strain Acanthamoeba castellanii Neff as a control. Briefly, the ability of these amoebae to bind to the three cell lines was calculated and supernatants were examined for cytotoxicity by measuring lactate dehydrogenase released as an estimate of cytotoxicity using a commercial detection kit. The three strains showed high adhesion and cytotoxicity levels when tested in the three cell lines. This study demonstrates the ability of these amoebae to degrade any of the tested cell lines. To the best of our knowledge, this is the first report of the in vitro effects of acanthamoebae on osteosarcoma cells.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Martín-Navarro CM,Lorenzo-Morales J,Machín RP,López-Arencibia A,Valladares B,Piñero JE

doi

10.1016/j.exppara.2009.10.011

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

85-8

issue

1

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(09)00299-9

journal_volume

126

pub_type

杂志文章

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