Abstract:
:Entamoeba histolytica infection is associated with considerable morbidity and mortality in the form of intestinal and extraintestinal amoebiasis. No vaccine is yet available for amoebiasis. Heparan Sulphate Binding Proteins (HSBPs) from E. histolytica were evaluated for immunogenicity and protective efficacy in a Guinea pig model. Animals were immunized subcutaneously with 30μg of HSBP by three weekly inoculations. The immunogenicity of HSBP was determined by antibody response (IgG, IgM and IgA), splenocyte proliferation assay and in vitro direct amoebicidal assay with splenic lymphocytes and monocytes from vaccinated and control animals. The efficacy of the vaccine was evaluated by challenge infection to vaccinated and control animals by intra-caecal inoculation of E. histolytica trophozoites and comparing gross and histopathological findings in caeca of these animals. HSBP was found to induce specific anti-amoebic response as seen by specific antibody production and direct amoebicidal activity of splenocytes. The vaccine also showed partial protection against challenge infection in vaccinated animals as shown by mild/absent lesions and histopathological findings.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Kaur U,Khurana S,Saikia UN,Dubey MLdoi
10.1016/j.exppara.2013.08.011subject
Has Abstractpub_date
2013-11-01 00:00:00pages
486-96issue
3eissn
0014-4894issn
1090-2449pii
S0014-4894(13)00232-4journal_volume
135pub_type
杂志文章abstract::Tapeworms are pervasive and globally distributed parasites that infect millions of humans and livestock every year, and are the causative agents of two of the 17 neglected tropical diseases prioritized by the World Health Organization. Studies of tapeworm biology and pathology are often encumbered by the complex life ...
journal_title:Experimental parasitology
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abstract::Rabbit antisera were raised against an 18 amino acid-long peptide that corresponds to the predicted sequence of the carboxy-terminal, nontriple helical region of the Haemonchus contortus 3A3 collagen gene. This sequence is highly conserved and diagnostic for members of the col-l collagen family, which includes the 3A3...
journal_title:Experimental parasitology
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abstract::The search for novel therapeutic candidates against animal trypanosomiasis is an ongoing scientific endevour because of the negative impacts of the disease to the African livestock industry. In this study, the in vivo therapeutic potentials of phytol toward Trypanosoma congolense infection and the inhibitory effects o...
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.06.017
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1997.4159
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1110
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.04.002
更新日期:2005-08-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(89)90178-1
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1998.4318
更新日期:1998-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2010.09.004
更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exppara.2007.07.005
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abstract::Visceral Leishmaniasis is an endemic disease in Brazil caused by Leishmania infantum chagasi and its main vector species is the sand fly Lutzomyia longipalpis. Epidemiological studies have used conventional PCR techniques to measure the rate of infection of sand flies collected in the field. However, real-time PCR can...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00
abstract::Schistosomiasis remains a global health problem. In the Mekong river basin, approximately 80,000 people are at risk of infection by Schistosoma mekongi. The parasite's eggs become entrapped in the host's organs and induce massive inflammation, contributing to the pathogenesis of schistosomiasis. In addition, egg antig...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.07.002
更新日期:2018-08-01 00:00:00