Abstract:
:Leishmania donovani, causative organism for visceral leishmaniasis, is responsible for considerable mortality and morbidity worldwide. Generation of drug-resistant variants continue to challenge the chemotherapy, the mainstay to fight the disease. The aim of current study was proteomic profiling of wild type (Ld-Wt) and arsenite-resistant (Ld-As20) L. donovani. Significant differences in protein profiles were observed between Ld-As20 and its parent Ld-Wt strain. Proteomic analysis of 158 spots from Ld-Wt and 144 spots from, Ld-As20 identified 77 and 74 protein entries, respectively, through MALDI-TOF/TOF based mass spectrometry and database search. A shift in the isoelectric point of few proteins was observed both in Ld-Wt and Ld-As20, which raises the possibility of continuous arsenite stress, resulting in the differences in the protein profiles of drug-resistant strain from its parent wild type strain. The comparative proteomic data holds the key for elucidation of the multifactorial and complex drug resistance mechanism, like arsenite resistance, in the parasite.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Sharma S,Singh G,Chavan HD,Dey CSdoi
10.1016/j.exppara.2009.08.003subject
Has Abstractpub_date
2009-12-01 00:00:00pages
369-76issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(09)00217-3journal_volume
123pub_type
杂志文章abstract::The mechanisms underlying metronidazole (MTZ) resistance in Giardia duodenalis have been associated with decreased activity of the enzymes implicated in its activation including nitroductase-1, thioredoxin reductase and pyruvate-ferredoxin oxidoreductase (PFOR). MTZ activation generates radicals that can form adducts ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.09.004
更新日期:2018-11-01 00:00:00
abstract::Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0096
更新日期:1996-11-01 00:00:00
abstract::Both the Weybridge strain and a mixture of six laboratory and field strains of Eimeria maxima have been attenuated by selection for early maturation of oocysts during serial passage in chickens. The prepatent times of the resultant precocious lines produces after selection were reduced from approximately 120 hr to les...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90152-9
更新日期:1986-04-01 00:00:00
abstract::The intestine of Haemonchus contortus is hypersensitive to the effects of the anthelmintic fenbendazole (FBZ). The effects are postulated to stem from disruption of microtubules and interference with apical secretory vesicle transport, followed by release of digestive enzymes into the intestinal cell cytoplasm. Here, ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(02)00135-2
更新日期:2002-08-01 00:00:00
abstract::Leishmaniasis, a neglected tropical disease (NTD) causes major health problems in the tropical and subtropical world. Most of the antileishmanial modern therapies with different formulations of pentavalent antimonials, Miltefosine, Amphotericin B etc. are not satisfactory in recent times due to high toxicity to the ho...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.08.009
更新日期:2014-11-01 00:00:00
abstract::Amoebae from the genus Acanthamoeba are facultative pathogens of humans and other animals. In humans they most frequently infect the eye causing a sight threatening infection known as Acanthamoeba keratitis (AK), and also cause an often fatal encephalitis (GAE). A mannose-binding protein (MBP) has been identified as b...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.07.009
更新日期:2018-09-01 00:00:00
abstract::The population structure of original Babesia bigemina isolates and reference strains with a defined phenotypic profile was assessed using 18S rRNA and rap-1c genes. Two reference strains, BbiS2P-c (virulent) and BbiS1A-c (low virulence), were biologically cloned in vitro. The virulence profile of the strains and clone...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2014.03.016
更新日期:2014-06-01 00:00:00
abstract::Nitric oxide (NO) has diverse biological functions. Numerous studies have documented NO's biosynthetic pathway in a wide variety of organisms. Little is known, however, about NO production in intraerythrocytic Plasmodium falciparum. Using diaminorhodamine-4-methyl acetoxymethylester (DAR-4M AM), a fluorescent indicato...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.04.014
更新日期:2008-09-01 00:00:00
abstract::Rabbit antisera were raised against an 18 amino acid-long peptide that corresponds to the predicted sequence of the carboxy-terminal, nontriple helical region of the Haemonchus contortus 3A3 collagen gene. This sequence is highly conserved and diagnostic for members of the col-l collagen family, which includes the 3A3...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(90)90098-w
更新日期:1990-02-01 00:00:00
abstract::Leishmania is an obligate intracellular protozoan parasite that infects cells of the reticulo-endothelial system. Host defences against Leishmania include fever and oxidant production, and the parasite has developed a number of defence mechanisms to neutralize the host response. The Leishmania donovani A2 family of pr...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.06.001
更新日期:2012-10-01 00:00:00
abstract::The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2001.4622
更新日期:2001-06-01 00:00:00
abstract::Though parasite cyclophilins are promising new drug targets, Eimeria tenella cyclophilins have not been characterized yet. Here, we describe an 89kDa cyclophilin, designated EtCYP89. It is expressed throughout the developmental cycle of E. tenella, both in the intracellular stages in chicken and in extracellular sporu...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.07.013
更新日期:2008-02-01 00:00:00
abstract::Here we describe the cloning and characterization of the Schistosoma mansoni Alkaline Phosphatase (SmAP), previously identified in the tegument of adult worms. SmAP encodes a complete sequence composed of 536 amino acids containing an N-terminal signal peptide, five N-glycosylation sites, and a GPI anchor signal, simi...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.07.008
更新日期:2011-11-01 00:00:00
abstract::Murine infection with Taenia crassiceps cysticerci is used as an experimental model for human and animal cysticercosis. In this infection parasites can be found associated with an inflammatory infiltrate enriched with macrophages. Experimental evidence exists supporting a role for either NO-producing classically activ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.09.014
更新日期:2013-11-01 00:00:00
abstract::Due to the occurrence of genetic recombination, a reliable and discriminatory method to genotype Cryptosporidium isolates at the intra-species level requires the analysis of multiple loci, but a standardised scheme is not currently available. A workshop was held at the Robert Koch Institute, Berlin in 2016 that gather...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2018.06.004
更新日期:2018-08-01 00:00:00
abstract::The extracellular metalloproteinases of the insect trypanosomatid Crithidia guilhermei were characterized through the incorporation of different protein substrates (gelatin, casein, haemoglobin, and bovine serum albumin) into SDS-PAGE. Two gelatinases (60 and 80 kDa) showed ability to degrade casein as well and a 67-k...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(03)00037-7
更新日期:2002-11-01 00:00:00
abstract::Genetic cross is a powerful tool for studying malaria genes contributing to drug resistance, parasite development, and pathogenesis. Cloning and identification of recombinant progeny (RP) is laborious and expensive, especially when a large proportion of progeny derived from self-fertilization are present in the unclon...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.10.011
更新日期:2013-01-01 00:00:00
abstract::Toxoplasma gondii is responsible for fetopathy in farm animals and humans and severe disease in immunocompromised individuals (i.e. AIDS patients). Effective vaccines, inducing protective and long-lasting immunity to this global parasite, are still desired. In the work, we evaluated the immunogenic and immunoprotectiv...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.07.003
更新日期:2008-02-01 00:00:00
abstract::An NADP-linked glutamate dehydrogenase (EC 1.4.1.4) was found in the soluble fraction of Trichomonas vaginalis. Its molecular weight was about 230,000 (gel filtration). The enzyme, partially purified by diafiltration and hydroxyapatite column chromatography, was heat stable (1 hr at 57 C). It catalyzed both the aminat...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(88)90007-0
更新日期:1988-10-01 00:00:00
abstract::The contribution of mononuclear phagocytes to host resistance against acute Chagas disease has been studied in vivo in a rat model inoculated with Trypanosoma cruzi, Y strain. Acute T. cruzi infection triggered a dramatic increase (937%) in peripheral blood monocyte number at day 12 of infection. At this point, histol...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2000.4576
更新日期:2001-01-01 00:00:00
abstract::This paper describes the in vitro multiplication process of Babesia bigemina sporokinetes in a cell line (IDE8) from Ixodes scapularis ticks. The inoculum was obtained from hemolymph of engorged females of Rhipicephalus (Boophilus) microplus ticks naturally infected with B. bigemina. These ticks had been fed on calves...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.03.011
更新日期:2009-07-01 00:00:00
abstract::The stability of the gene encoding TSA417, a 72-kDa variant-specific surface protein (VSP) produced by trophozoites of Giardia intestinalis isolate WB-C6, was investigated in isolates of similar (Assemblage A / Group I) or distinct (Assemblage A / Group II) genotype. Using primers specific for the WB-C6 tsa417 gene, D...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1998.4325
更新日期:1998-11-01 00:00:00
abstract::The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F. hepatica, rats were treated with a single 100mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.12.015
更新日期:2008-05-01 00:00:00
abstract:PURPOSE:Praziquantel (PZQ) is the conventional antibilharzial agent. Nevertheless, no antibilharzial prophylactic agents or 100% curable therapy approved and no reported data about use of human CD34+ Umbilical Cord Blood Stem Cells (CD34+UCBSCs) or Wharton Jelly Mesenchymal Stem Cells (WJMSCs) in prevention and/or comp...
journal_title:Experimental parasitology
pub_type: 杂志文章,评审
doi:10.1016/j.exppara.2020.107938
更新日期:2020-10-01 00:00:00
abstract::Coccidiosis is a major parasitic disease of poultry industry and an ideal vaccine should induce long-lasting cross-species protective immunity. Broiler chickens (Cobb 500) were inoculated with single, double or triple injections of a synthetic peptide (derived from sequences of Eimeria acervulina and Eimeria tenella a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.03.026
更新日期:2005-08-01 00:00:00
abstract::Rosetting is a parasite adhesion phenotype associated with severe malaria in African children. Why parasites form rosettes is unknown, although enhanced invasion or immune evasion have been suggested as possible functions. Previous work showed that rosetting does not enhance parasite invasion under standard in vitro c...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.11.007
更新日期:2006-04-01 00:00:00
abstract::Experimental infections of chimpanzees with Onchocerca volvulus and cattle with Onchocerca ochengi provide model systems for research in human onchocerciasis. These infections share many similarities from the standpoint of parasite biology, but little is known about the comparability of immune responses in the two sys...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.2000.4487
更新日期:2000-03-01 00:00:00
abstract::Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The anal...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2007.09.009
更新日期:2008-03-01 00:00:00
abstract::A new type of cell has been identified in cercariae of Schistosoma mansoni. The perikarya (cell bodies) of these cells were located in the body (midsegment), in an area oral to the acetabulum (ventral sucker). Cytoplasmic processes extending from the perikarya ramified throughout the parenchyma of the anterior organ (...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90132-3
更新日期:1986-02-01 00:00:00
abstract::Cutaneous lesions elicited in guinea pigs by primary and secondary feeding populations of the argasid tick, Ornithodorus tartakovskyi, were analyzed by light and electron microscopy. Small clusters of basophils appeared at primary bite sites within 24 hr of tick attachment, and by 72 hr constituted approximately 11% o...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(83)90058-9
更新日期:1983-10-01 00:00:00