Abstract:
:Amoebae from the genus Acanthamoeba are facultative pathogens of humans and other animals. In humans they most frequently infect the eye causing a sight threatening infection known as Acanthamoeba keratitis (AK), and also cause an often fatal encephalitis (GAE). A mannose-binding protein (MBP) has been identified as being important for Acanthamoeba infection especially in AK. This lectin has previously been characterized from Acanthamoeba castellanii as consisting of multiple 130 kDa subunits. MBP expression correlates with pathogenic potential and is expressed in a number of Acanthamoeba species. Here we report the purification of a similar lectin from Acanthamoeba culbertsoni and the production of a monoclonal antibody to it. The A. culbertsoni MBP was isolated by affinity chromatography using α-D-mannose agarose and has an apparent molecular weight of 83 kDa. The monoclonal antibody is an IgM that is useful in both western blots and immunofluorescence. We expect that this antibody will be useful in the study of the pathology of A. culbertsoni and in its identification in clinical samples.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Kang AY,Park AY,Shin HJ,Khan NA,Maciver SK,Jung SYdoi
10.1016/j.exppara.2018.07.009subject
Has Abstractpub_date
2018-09-01 00:00:00pages
19-24eissn
0014-4894issn
1090-2449pii
S0014-4894(18)30166-8journal_volume
192pub_type
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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