Abstract:
:A murine model system was developed to study the induction and mechanism of protective immunity to L3 of Strongyloides stercoralis. L3 were implanted in BALB/cByJ mice in diffusion chambers constructed with 0.1- or 2.0-microns-pore-size membranes. Parasites survived equally well regardless or membrane type for 7 days, after which larval survival decreased in diffusion chambers constructed with 2.0-microns-pore-size membranes, which allowed host cells to enter. Survival of S. stercoralis L3 in diffusion chambers implanted in mice was assayed after immunization with live, heat-killed, and homogenized L3. Optimal immunization was achieved with 10,000 live L3, whereby immunized mice eliminated 97% of the larvae either contained within diffusion chambers or free within the tissues of the mouse by 24 hr postinfection. Sera from immunized mice had elevated levels of IgG1, IgM, and IgA parasitic-specific antibody; IgM was the only antibody isotype that recognized surface antigens of L3. Larvae were not killed in immunized mice if contact between host cells and the parasites was prevented. In the peripheral blood and diffusion chamber fluid of immunized mice, eosinophil levels were significantly higher when compared to the levels found in control mice. The rodent model developed in the present study has thus demonstrated that virtually complete immunity can be induced to the L3 of S. stercoralis and that larval killing was found to be associated with the presence of both specific antibody and eosinophils.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Abraham D,Rotman HL,Haberstroh HF,Yutanawiboonchai W,Brigandi RA,Leon O,Nolan TJ,Schad GAdoi
10.1006/expr.1995.1036subject
Has Abstractpub_date
1995-03-01 00:00:00pages
297-307issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(85)71036-3journal_volume
80pub_type
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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更新日期:1989-01-01 00:00:00
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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doi:10.1016/j.exppara.2005.02.014
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.03.025
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(92)90135-w
更新日期:1992-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exppara.2006.09.019
更新日期:2007-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2013.01.003
更新日期:2013-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0068
更新日期:1996-07-01 00:00:00
abstract::Toxoplasmosis is a widely distributed disease caused by the protozoan Toxoplasma gondii that is mainly transmitted orally. Once ingested, the parasite crosses the intestinal barrier to reach the blood and lymph systems to migrate to other regions of the host. The objective of this study was to evaluate the changes in ...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0096
更新日期:1996-11-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
abstract::Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (TcI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study ...
journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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journal_title:Experimental parasitology
pub_type: 杂志文章
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