In vitro growth, cytopathic effects and clearance of monolayers by clinical isolates of Balamuthia mandrillaris in human skin cell cultures.

Abstract:

:Balamuthia mandrillaris is a free-living ameba (FLA) that has been isolated or its DNA identified in soil, dust and water. It causes a fatal central nervous system infection in humans and animals. Although it is environmental as Acanthamoeba and Naegleria fowleri, the two other free-living amebae that also cause CNS infections in humans and other animals, Balamuthia does not feed on bacteria as the other FLA. In the laboratory, it can be grown on a variety of mammalian cell cultures. In this study we examined the ability of three different Balamuthia isolates to grow on several different human skin cell cultures including the WT/A keratinocyte cell cultures. A corneal isolate of Acanthamoeba castellanii was used for comparison.

journal_name

Exp Parasitol

authors

Yera H,Dupouy-Camet J,Jackson JW,Sriram R,Sweat S,Goldstein JM,Visvesvara GS

doi

10.1016/j.exppara.2015.05.004

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

61-7

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(15)00128-9

journal_volume

156

pub_type

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