Identification of α-11 giardin as a flagellar and surface component of Giardia lamblia.

abstract：:Aedes albopictus mosquito is an opportunistic blood feeder and has a broad host range. The feeding behavior and habits of this mosquito are liable to increase the transmission potential of arboviruses. The survival and fecundity in A. albopictus fed on different hosts and post-blood meal provision of sugar were invest...

journal_title：Experimental parasitology

authors： Xue RD,Ali A,Barnard DR

更新日期：2008-06-01 00:00:00

abstract：:The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (Schistosoma mansoni, S. japonicum, and S. haematobium) with 3H- and 14C-labeled 2-deoxy-D-glucose. Subsequent column chromatographic separation of the neutral [3H]2DG and [14C]2DG from the 3H- and 14C-labeled 2-de...

journal_title：Experimental parasitology

authors： Cornford EM,Fitzpatrick AM

更新日期：1987-12-01 00:00:00

abstract：:A cell-free culture system for Cryptosporidium parvum was analysed using scanning electron microscopy (SEM) to characterise life cycle stages and compare gene expression in cell-free culture and cell culture using HCT-8 cells. Cryptosporidium parvum samples were harvested at 2 h, 8 h, 14 h, 26 h, 50 h, 74 h, 98 h, 122...

journal_title：Experimental parasitology

authors： Yang R,Elankumaran Y,Hijjawi N,Ryan U

更新日期：2015-06-01 00:00:00

abstract：:A Schistosoma mansoni G-protein coupled receptor (SmGPCR) was previously cloned and shown to be activated by the biogenic amine, histamine. Here we report a first investigation of the receptor's subunit organization, tissue distribution and expression levels in different stages of the parasite. A polyclonal antibody w...

journal_title：Experimental parasitology

authors： El-Shehabi F,Vermeire JJ,Yoshino TP,Ribeiro P

更新日期：2009-05-01 00:00:00

abstract：:Hemocytes derived from a strain (13-16-R1) of Biomphalaria glabrata resistant to Schistosoma mansoni were significantly more likely to bind untreated latex beads than hemocytes from the schistosome-susceptible M line strain. Beads preincubated in 13-16-R1 plasma were more readily bound by both 13-16-R1 and M line hemo...

journal_title：Experimental parasitology

authors： Uchikawa R,Loker ES

更新日期：1992-09-01 00:00:00

abstract：:Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...

journal_title：Experimental parasitology

authors： Buckwitz D,Jacobasch G,Kuckelkorn U,Plonka A,Gerth C

更新日期：1990-04-01 00:00:00

abstract：:Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the para...

journal_title：Experimental parasitology

authors： Faro MJ,Perazzini M,Corrêa Ldos R,Mello-Silva CC,Pinheiro J,Mota EM,de Souza S,de Andrade Z,Júnior AM

更新日期：2013-06-01 00:00:00

abstract：:The existence of cholinergic receptors in the immune system cells is well documented. This study aimed to evaluate the acetylcholinesterase activity (AChE) in lymphocytes from rats infected with Trypanosoma evansi in acute and chronic phase disease. Twenty animals were infected with 10(6) trypomastigotes forms each an...

journal_title：Experimental parasitology

authors： Da Silva AS,Monteiro SG,Gonçalves JF,Spanevello R,Schmatz R,Oliveira CB,Costa MM,França RT,Jaques JA,Schetinger MR,Mazzanti CM,Lopes ST

更新日期：2011-02-01 00:00:00

abstract：:Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...

journal_title：Experimental parasitology

authors： Scharton-Kersten T,Caspar P,Sher A,Denkers EY

更新日期：1996-11-01 00:00:00

abstract：:It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days...

journal_title：Experimental parasitology

authors： Wen-Yue X,Jian Z,Tao-Li Z,Fu-Sheng H,Jian-Hua D,Ying W,Zhong-Wen Q,Li-Sha X

更新日期：2007-08-01 00:00:00

abstract：:The asexual development cycle of Plasmodium falciparum, a malarial parasite of humans, has been synchronized in culture by treating ring-stage parasites with aphidicolin, an inhibitor of DNA synthesis. Optimization of both the concentration of drug added to ring stage containing red blood cells and the duration of exp...

journal_title：Experimental parasitology

authors： Inselburg J,Banyal HS

更新日期：1984-02-01 00:00:00

abstract：:Stable RNAs have regions of primary sequence that are nearly identical in every member of the Plasmodium genus and not found in the host or in other common pathogens. Several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal RNA of Plasmodium species. Pr...

journal_title：Experimental parasitology

authors： Li J,Wirtz RA,McConkey GA,Sattabongkot J,Waters AP,Rogers MJ,McCutchan TF

更新日期：1995-09-01 00:00:00

abstract：:This report describes a molecular method for determining in a first step the generic composition of a nematode community and in a second step, the resistance of each species to benzimidazole (BZ). We first established a polymerase chain reaction (PCR) linked to a restriction fragment length polymorphism strategy using...

journal_title：Experimental parasitology

authors： Silvestre A,Humbert JF

更新日期：2000-08-01 00:00:00

abstract：:The cDNA of a Schistosoma japonicum myophilin-like protein was cloned, sequenced, and expressed in Escherichia coli as a recombined protein (rSj myophilin-like protein), and the protein was purified by affinity chromatography. The deduced amino acid sequences of the Sj myophilin-like protein showed significant homolog...

journal_title：Experimental parasitology

authors： Peng H,Song K,Huang C,Ye S,Song H,Hu W,Han Z,McManus DP,Zhao G,Zhang Q

更新日期：2008-05-01 00:00:00

abstract：:A cDNA encoding elastase was isolated from Steinernema carpocapsae by suppression subtractive hybridization and rapid amplification of 5' cDNA ends. The predicted protein contained a 19-aa signal peptide, a 44-aa N-terminal propeptide, and a 264-aa mature protein with a predicted molecular mass of 28,949 Da and a theo...

journal_title：Experimental parasitology

authors： Hao YJ,Montiel R,Nascimento G,Toubarro D,Simoes N

更新日期：2009-05-01 00:00:00

abstract：:Cryptosporidiosis, caused by the protozoan parasite Cryptosporidium, represents the major public health concern of water utilities in developed nations due to its small size, resistance to disinfection and ability to be shed in large numbers in faeces. In Australia, recreational access is not allowed on direct supply ...

journal_title：Experimental parasitology

authors： Loganthan S,Yang R,Bath A,Gordon C,Ryan U

更新日期：2012-08-01 00:00:00

abstract：:Resistance of human pathogens like Leishmania to drugs is a growing concern where the multidrug-resistant phenotype renders chemotherapy ineffective. The acquired resistance of Leishmania to antimony has promoted intense research on the mechanisms involved but the question has not been resolved yet. In this study we h...

journal_title：Experimental parasitology

authors： Singh N,Sundar S

更新日期：2017-05-01 00:00:00

abstract：:The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic struc...

journal_title：Experimental parasitology

authors： González-Robles A,Castañón G,Hernández-Ramírez VI,Salazar-Villatoro L,González-Lázaro M,Omaña-Molina M,Talamás-Rohana P,Martínez-Palomo A

更新日期：2008-07-01 00:00:00

abstract：:Parasites of the Leishmania genus require for the growth and viability the de novo synthesis of specific sterols as such as episterol and 5-dehydroepisterol because cholesterol, which is abundant in their mammalian hosts, does not fulfill the parasite sterol requirements. Squalene synthase catalyzes the first committe...

journal_title：Experimental parasitology

authors： Rodrigues JC,Urbina JA,de Souza W

更新日期：2005-12-01 00:00:00

abstract：:Indirect immunocytochemistry, in conjunction with confocal scanning laser microscopy and electron-microscopic immunogold labeling, has been used to localize neuropeptide and 5-hydroxytryptamine (5-HT) immunoreactivities (IRs) in the plerocercoid (scolex and surrounding blastocyst) of the trypanorhynch tapeworm, Grillo...

journal_title：Experimental parasitology

authors： Halton DW,Maule AG,Brennan GP,Shaw C,Stoitsova SR,Johnston CF

更新日期：1994-11-01 00:00:00

abstract：:Reliable analytical techniques to test growth-promoting and antimalarial efficacy on plasmodia are very important. Flow cytometry (FCM) offers the possibility to study developmental stages of intraerythrocytic growth of malaria parasites using nucleic acid staining. To analyze the growth of Plasmodium falciparum SYBR ...

journal_title：Experimental parasitology

authors： Izumiyama S,Omura M,Takasaki T,Ohmae H,Asahi H

更新日期：2009-02-01 00:00:00

abstract：:The present work evaluates the anti-Giardia activity of Syzygium aromaticum and its major compound eugenol. The effects were evaluated on parasite growth, adherence, viability and ultrastructure. S. aromaticum essential oil (IC(50)=134 μg/ml) and eugenol (IC(50)=101 μg/ml) inhibited the growth of G. lamblia. The essen...

journal_title：Experimental parasitology

authors： Machado M,Dinis AM,Salgueiro L,Custódio JB,Cavaleiro C,Sousa MC

更新日期：2011-04-01 00:00:00

abstract：:A limiting dilution assay (LDA) was developed for the quantification of Trypanosoma cruzi in the heart and blood of infected mice. Three groups of swiss mice were injected ip with "CL", "Colombiana," and "Y" strains. At 1-day intervals after infection, blood and the heart were removed. Serial blood dilutions in LIT me...

journal_title：Experimental parasitology

authors： Nunes MP,Coutinho SG,Louis JA,Souza WJ

更新日期：1990-02-01 00:00:00

abstract：:The population structure of original Babesia bigemina isolates and reference strains with a defined phenotypic profile was assessed using 18S rRNA and rap-1c genes. Two reference strains, BbiS2P-c (virulent) and BbiS1A-c (low virulence), were biologically cloned in vitro. The virulence profile of the strains and clone...

journal_title：Experimental parasitology

authors： Thompson C,Baravalle ME,Valentini B,Mangold A,Torioni de Echaide S,Ruybal P,Farber M,Echaide I

更新日期：2014-06-01 00:00:00

abstract：:Two strains of mice which share identical H-2 genes but differ in their genetic backgrounds were compared for their ability to resist infection with Trichinella spiralis. The two strains of mice, C3HeB/FeJ and AKR/J, share the H-2k haplotype which is associated with susceptibility to primary infection with T. spiralis...

journal_title：Experimental parasitology

authors： Wassom DL,Brooks BO,Cypess RH,David CS

更新日期：1983-04-01 00:00:00

abstract：:Leishmaniasis is one of the most neglected tropical infectious diseases in the world. The emergence of drug resistance and toxicity and the high cost of the available drugs with a lack of new anti-leishmanial drugs highlight the need to search for newer therapies with anti-leishmanial activities. Due to the mesenchyma...

journal_title：Experimental parasitology

authors： Bahrami S,Safari M,Razi Jalali MH,Ghorbanpoor M,Tabandeh MR,Rezaie A

更新日期：2021-01-04 00:00:00

abstract：:Schistosoma mansoni muscle fibers contract in response to L-glutamate in a dose-dependent manner (10(-6)-10(-3) M). L-aspartate and D-aspartate are likewise effective in eliciting contraction of the fibers. Mammalian glutamate receptor agonists produce little or no contraction at concentrations as high as 1 mM. In add...

journal_title：Experimental parasitology

authors： Miller CL,Day TA,Bennett JL,Pax RA

更新日期：1996-12-01 00:00:00

abstract：:This is the first report establishing the existence of glycolipids synthesized by plasmodia, in particular Plasmodium falciparum. Trophozoites, schizonts, gametocytes, and gametes were metabolically labeled in vitro with [3H]glucosamine, [3H]galactose, [3H]glucose, [3H]mannose, [3H]fucose, [32P]inorganic phosphate, or...

journal_title：Experimental parasitology

authors： Sherwood JA,Spitalnik SL,Aley SB,Quakyi IA,Howard RJ

更新日期：1986-08-01 00:00:00

abstract：:To understand the genetic basis of virulence, gene expression profiles of a temperature-sensitive clone (NCts-8, relatively avirulent) and its wild type (NC-1) of Neospora caninum were characterized and compared using a high-density microarray with approximately 63,000 distinct oligonucleotides. This microarray consis...

journal_title：Experimental parasitology

authors： Li RW,Tuo W

更新日期：2011-12-01 00:00:00

abstract：:The oocysts of Eimeria tenella, one of the most pathogenic of several species causing chicken coccidiosis, are difficult to distinguish microscopically from several other infective Eimeria species. One copy of a gene coding for 5S ribosomal RNA has been cloned from E. tenella and sequenced. A coding region of 120 nucl...

journal_title：Experimental parasitology

authors： Stucki U,Braun R,Roditi I

更新日期：1993-02-01 00:00:00