Abstract：
:The existence of cholinergic receptors in the immune system cells is well documented. This study aimed to evaluate the acetylcholinesterase activity (AChE) in lymphocytes from rats infected with Trypanosoma evansi in acute and chronic phase disease. Twenty animals were infected with 10(6) trypomastigotes forms each and 10 were used as negative controls. The two groups of inoculated rats were formed according to the degree of parasitemia and the period post-infection (PI). Group A: rats with 4 days PI and between 24 and 45 parasites/field (1000×); group B: rats with 30 days PI and parasitemia with jagged peaks between 0 and 1 parasites/field; group C: not-infected animals. At 4 days PI (acute phase) and 30 days PI (chronic phase) the rats were anesthetized to collect blood for hemogram and separation of lymphocytes. After separation, the AChE activity was measured in lymphocytes. It was observed that the number of lymphocytes increased significantly in group A compared to group C. The activity of AChE in lymphocytes significantly increased in acute phase and decreased in chronic phase in the infected rats when compared to not-infected (P<0.05). Statistical analysis showed a positive correlation between the number of lymphocytes and AChE activity in lymphocytes in 4 days PI (r(2): 0.59). Therefore, the infection by T. evansi influences AChE activity in lymphocytes of rats indicating changes in the responses of cholinergic system in acute phase, possibly due to immune functions performed by these enzymes.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Da Silva AS,Monteiro SG,Gonçalves JF,Spanevello R,Schmatz R,Oliveira CB,Costa MM,França RT,Jaques JA,Schetinger MR,Mazzanti CM,Lopes STdoi
10.1016/j.exppara.2010.10.017subject
Has Abstractpub_date
2011-02-01 00:00:00pages
475-80issue
2eissn
0014-4894issn
1090-2449pii
S0014-4894(10)00336-Xjournal_volume
127pub_type
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