Abstract:
:Phospholipid metabolism of the microsporidian Encephalitozoon cuniculi, an obligate intracellular parasite, has been investigated. Labeled precursor incorporation experiments have shown that phosphatidylserine decarboxylase and phosphatidylethanolamine N-methyltransferase are more active in cells infected by E. cuniculi than in uninfected cells. In contrast, no difference was observed in the activity of Kennedy pathway's enzymes, the mammalian pathway. This suggests the occurrence in microsporidia of a bacteria- and fungi-typical pathway for phospholipid synthesis, which is supported by the identification of two genes implicated in this pathway, the cds gene encoding the key enzyme CDP-diacylglycerol synthase (E.C. 2.7.7.41) and the pss gene for CDP-alcohol phosphatidyltransferase. The pss gene could encode phosphatidylserine synthase (E.C. 2.7.8.8.), which catalyses the de novo synthesis of phosphatidylserine in bacteria and fungi. The complete CDP-diacylglycerol synthase messenger has been isolated and shows very short 5' and 3' untranslated regions. This is strong evidence for the functionality of a metabolic pathway which could be a potential target against microsporidia which infect humans.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
El Alaoui H,Bata J,Peyret P,Vivarès CPdoi
10.1006/expr.2001.4635keywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
171-9issue
4eissn
0014-4894issn
1090-2449pii
S0014-4894(01)94635-1journal_volume
98pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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