Abstract：
:Partial nucleotide sequences of large subunit ribosomal RNA (LSU rRNA) coding genes of Theileria parva and T. taurotragi were determined. Alignment of the T. parva and T. taurotragi sequences revealed species-specific regions within the LSU rRNA genes. A major species-variable region, located between the first and second conserved secondary structure domains of LSU rRNA, was identified. The sequence of the major species-variable region was determined for T. annulata, T. buffeli, and an unclassified Theileria species isolated from buffalo. Species-specific oligonucleotides complementary to LSU rRNA sequences were designed for five species of Theileria. The oligonucleotide probes discriminated between Theileria species when hybridized to amplified parasite LSU rRNA genes or directly to parasite rRNA. Three species-specific oligonucleotides derived from nonoverlapping sequences were developed for each of T. parva and T. taurotragi. A simplified procedure was developed in which T. parva- and T. taurotragi-specific oligonucleotides were used to discriminate the two species under nonstringent hybridization and washing conditions. Use of the nonstringent conditions also increased the sensitivity of target detection. Comparison of Theileria species-specific oligonucleotides derived from large subunit and small subunit rRNA genes demonstrated that, for closely related species, probes derived from LSU rRNA sequences had improved specificity.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Bishop R,Allsopp B,Spooner P,Sohanpal B,Morzaria S,Gobright Edoi
10.1006/expr.1995.1012subject
Has Abstractpub_date
1995-02-01 00:00:00pages
107-15issue
1eissn
0014-4894issn
1090-2449pii
S0014-4894(85)71012-0journal_volume
80pub_type
杂志文章abstract：:The present study incorporates the findings on in vitro and in vivo antifilarial activity in the marine sponge, Haliclona oculata using an experimental rodent infection of human lymphatic filarial parasite, Brugia malayi. The in vitro antifilarial action was determined on both adult female worms as well as microfilari...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.01.009
更新日期：2012-04-01 00:00:00
abstract：:The present study aimed to evaluate the acaricidal efficacy of fluazuron (2.5 mg/kg), administered as a pour-on, in comparison to an injectable formulation containing fluazuron (1.6 mg/kg) + ivermectin (0.63 mg/kg), against Rhipicephalus (Boophilus) microplus in naturally and experimentally infested cattle. Two studie...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2015.02.004
更新日期：2015-06-01 00:00:00
abstract：:Acanthamoeba is a free-living amoeba genus that causes several diseases namely, amoebic keratitis which is a painful sight threatening eyes disease. Its treatment is difficult and the exploration for new drugs is very important. The main objective of the present study was to evaluate the chemical composition of the Es...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2017.10.011
更新日期：2017-12-01 00:00:00
abstract：:Studies have shown that symptomatic infection by Giardia lamblia causes acute or chronic diarrhea, dehydration, abdominal pain and malabsorption, leading to undernutrition and weight loss. The aim of the present study was to evaluate the effects of giardiasis and its combination with a low-protein diet on the intestin...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.04.016
更新日期：2012-08-01 00:00:00
abstract：:The effect of experimental exposure of Bradybaena similaris by Eurytrema coelomaticum on the cholesterol and triglycerides total levels circulating in the hemolymph and the neutral lipids in the digestive gland-gonad (DGG) complex of the host snail were studied. In this study, snails were dissected after one, two, and...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2014.06.010
更新日期：2014-09-01 00:00:00
abstract：:Rhoptries are involved in host cell invasion and rhoptry polypeptides, including the Babesia bigemina rhoptry-associated protein-1 (RAP-1), are targets for protective immune responses. Polyclonal antisera produced against isolated rhoptries is directed predominantly against RAP-1 and reacts with both the merozoite and...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1142
更新日期：1995-12-01 00:00:00
abstract：:There are alterations in the proteins synthesized during different stages of development of Schistosoma mansoni. The protein profiles at different stages were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When stained by Coomassie blue, no significant differences were seen in protein profiles ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(87)90177-9
更新日期：1987-06-01 00:00:00
abstract：:With the aim of determining selectivity and the possible target(s) of nitroheteroaryl-1,3,4-thiadiazoles considered as possible leads for the development of anti-leishmanial agents, we studied 5-nitroimidazole, 5-nitrofuran and 5-nitrothiophene analogs of N-substituted-piperazinyl-1,3,4-thiadiazoles. We investigated 2...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.12.004
更新日期：2009-04-01 00:00:00
abstract：:Vero cells have been used successfully in Toxoplasma gondii maintenance. Medium supplementation for culture cells with fetal bovine serum is necessary for cellular growth. However, serum in these cultures presents disadvantages, such as the potential to induce hypersensitivity, variability of serum batches, possible p...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2012.01.005
更新日期：2012-04-01 00:00:00
abstract：:Proteasomes are multi-subunit proteases involved in several mechanisms and thought to contribute to the regulation of cellular homeostasis. Here, we report for the first time biochemical evidence for the existence of a ubiquitin-proteasome proteolytic pathway in this parasite. Proteasomes from both cercariae and adult...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.01.002
更新日期：2005-04-01 00:00:00
abstract：:Many methods have been proposed for removing contaminating host cells from mouse peritoneal exudates infected with Toxoplasma gondii tachyzoites. Of these, eight established methods were compared. They were density gradients, sonication and trypsin digestion, differential centrifugation, haemolysin digestion, filtrati...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(84)90080-8
更新日期：1984-04-01 00:00:00
abstract：:Philasterides dicentrarchi is a histophagous scuticociliate infecting Korean olive flounder farms in Jeju Island, South Korea, where it causes significant economic losses. However, the route of entry of these parasites in olive flounder is currently unknown. In the present study, we attempted experimental infection wi...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.12.007
更新日期：2012-03-01 00:00:00
abstract：:One of the most fascinating aspects of the Entamoeba histolytica trophozoite ultrastructure is the lack of a typical secretory pathway, particularly of rough endoplasmic reticulum and Golgi system, in a cell with such a high secretory activity. Here, we describe the isolation of amoeba cell structures containing ER-ty...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.04.002
更新日期：2005-08-01 00:00:00
abstract：:Visceral Leishmaniasis is an endemic disease in Brazil caused by Leishmania infantum chagasi and its main vector species is the sand fly Lutzomyia longipalpis. Epidemiological studies have used conventional PCR techniques to measure the rate of infection of sand flies collected in the field. However, real-time PCR can...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.08.010
更新日期：2011-11-01 00:00:00
abstract：:Three clones of Entamoeba histolytica (L-6, C93, C919) were isolated by mutagenesis with ethyl methanesulfonate from the axenic strain HM1:IMSS and were studied for adherence, cytolytic, and soluble galactose inhibitable lectin activity. Avirulent clones adhered to and killed fewer Chinese hamster ovary cells than HM1...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(87)90157-3
更新日期：1987-04-01 00:00:00
abstract：:Toxoplasma gondii invades any nucleated cell, but different replication speed and effects on survival/apoptosis processes have been found depending on cell type. There are scarce and controversial results regarding the effect of this parasite on host cell apoptosis within the brain. The invasion and replication of T. ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2013.03.010
更新日期：2013-06-01 00:00:00
abstract：:Aedes aegypti mosquitoes salivate during intradermal probing of vertebrate prey before ingesting blood (Griffiths and Gordon 1952). Nonsalivating mosquitoes locate blood more slowly; this difference was ascribed to an anti-platelet activity found in the mosquito's saliva (Ribeiro et al. 1984). Mosquitoes infected with...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/s0014-4894(85)80029-1
更新日期：1985-08-01 00:00:00
abstract：:A comparative study of cell surface characteristics of pathogenic and nonpathogenic promastigotes of Leishmania braziliensis, NR and LBY strains, respectively, was carried out by means of concanavalin A agglutination and labeling with concanavalin A-fluorescein isothiocyanate, concanavalin A-ferritin, and cationized f...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(85)90071-2
更新日期：1985-04-01 00:00:00
abstract：:Larvae of Rhinoestrus spp. (Diptera, Oestridae) infect nasal and sinus cavities of horses, causing a nasal myiasis characterized by severe respiratory distress. Presently, the diagnosis of horse nasal botfly relies on the observation of clinical signs, on the post mortem retrieval of larvae or on molecular assays perf...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.11.014
更新日期：2010-04-01 00:00:00
abstract：:Infection with the protozoan Leishmania donovani in humans is usually subclinical. Parasites probably persist for the life of the host and the low-level infection is controlled by the cellular immune response. To better understand the mechanisms related to the control of infection, we studied the evolution and archite...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.2001.4640
更新日期：2001-09-01 00:00:00
abstract：:Mature males and females of Schistosoma mansoni were incubated for 24 hr in medium containing [3H]cholesterol. Worms thus labeled were paired for 24 hr with unlabeled partners, in vitro or in vivo by surgical implantation into hamsters. Controls consisted of additional unlabeled worms that did not pair. Scintillation ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(86)90189-x
更新日期：1986-06-01 00:00:00
abstract：:Acanthamoeba are free-living amoebae found in most environments that can cause brain and corneal infections. To infect humans, these pathogens must interact with host cells and the extracellular matrix (ECM). In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyze...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.08.004
更新日期：2010-09-01 00:00:00
abstract：:Exponential weight gain equations and growth rates were determined during larval development of Brachymeria lasus reared aseptically on chemically defined media with varying amino acid and glucose levels. The rate of weight gain increased from 0 to 2% dietary glucose and mean growth rate increased from 160 to 260 micr...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/0014-4894(83)90028-0
更新日期：1983-06-01 00:00:00
abstract：:The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic struc...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.04.004
更新日期：2008-07-01 00:00:00
abstract：:Okuda, K., Esteva, M., Segura, E. L., and Bijovsky, A. T. 1999. The cytostome of Trypanosoma cruzi epimastigotes is associated with the flagellar complex. Experimental Parasitology 92, 223-231. Proliferative forms of Trypanosoma cruzi, amastigotes and epimastigotes, have a cytostome, a specialized structure formed by ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1999.4419
更新日期：1999-08-01 00:00:00
abstract：:Members of the zinc-regulated transporter/iron-regulated transporter-like protein (ZIP) family of proteins transport metal ions across cell membranes. Genes encoding ZIPs are present in the genomes of schistosomes. Here, we describe molecular characterisation of six ZIPs (SjZIPA-F) from Schistosoma japonicum. Quantita...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2011.01.002
更新日期：2011-04-01 00:00:00
abstract：:Plasma butyrylcholinesterase (BChE) hydrolyzes ester-containing compounds such as succinylcholine, as well as acting as a scavenger against neurotoxic organophosphates (OPs). We previously found that Nippostrongylus brasiliensis infection makes rats more susceptible to OP toxicity by decreasing serum paraoxonase-1 (PO...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2009.02.009
更新日期：2009-06-01 00:00:00
abstract：:Excystation of Giardia lamblia entails differentiation of dormant cysts into parasitic trophozoites. Despite its importance for infection, this transformation is not understood at the cellular or molecular levels. In these studies, we report that excystation entails detection of environmental stimuli across the tough ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1998.4246
更新日期：1998-03-01 00:00:00
abstract：:In this work we demonstrated that promastigotes of Leishmania amazonensis exhibit an Mg-dependent ecto-ATPase activity, which is stimulated by heat shock. The Mg-dependent ATPase activity of cells grown at 22 and 28 degrees C was 41.0+/-5.2 nmol Pi/h x 10(7)cells and 184.2+/-21.0 nmol Pi/h x 10(7)cells, respectively. ...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2008.01.003
更新日期：2008-05-01 00:00:00
abstract：:It is generally accepted that a majority of individuals infected by Entamoeba histolytica do not develop symptomatic disease. However, the parasite and the host factors contributing to the development of the disease, remain undetermined. It is also unclear why certain individuals develop extra-intestinal amebiasis wit...
journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2005.02.015
更新日期：2005-07-01 00:00:00
