Abstract：
:Free Living Amoebae (FLA) are considered ubiquitous. FLAs may infect various biological organisms which act as reservoir hosts. Infected freshwater fishes can pose a public health concern due to possible human consumption. This study aims to identify possible pathogenic FLAs present in freshwater fishes. Seventy five (75) Oreochromis niloticus were studied for the presence of FLAs. Fish organs were suspended in physiologic saline pelleted and cultured in non-nutrient agar (NNA) lawned with Escherichia coli and were incubated in 33 °C for 14 days. Eighteen (18) fish gills and nineteen (19) fish intestine samples presented with positive growth. Trophozoites and cystic stages of FLAs were subcultured until homogenous growth was achieved. Cells were harvested from cultured plates and DNA was extracted using Chelex resin. DNA was subjected to polymerase chain reaction using universal forward primer EukA and reverse primer EukB targeting the 18s RNA. Of the 37 plates that presented with positive amoebic growth, 9 samples showed the presence of DNAs and were sent for further purification and sequencing. Basic Local Alignment Search Tool (BLAST) results showed that protists isolated from fish organs in Lake Taal include: Eocercomonas (HM536152), Colpoda steinii (KJ607915) and Vermamoeba vermiformis (KC161965). The results showed that fresh-water fishes can harbour FLAs in the gut. It is proposed that freshwater reservoirs utilized for aquaculture be monitored for the presence of FLAs and extensive study be conducted on the pathogenicity of bacterial endosymbionts and infecting viruses to its mammalian and non-mammalian host.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Milanez GD,Masangkay FR,Thomas RC,Ordona MOGO,Bernales GQ,Corpuz VCM,Fortes HSV,Garcia CMS,Nicolas LC,Nissapatorn Vdoi
10.1016/j.exppara.2017.09.009subject
Has Abstractpub_date
2017-12-01 00:00:00pages
201-206eissn
0014-4894issn
1090-2449pii
S0014-4894(17)30409-5journal_volume
183pub_type
杂志文章abstract：:Schistosoma mansoni cercariae are stimulated by 2-tetradecenoic acid (TDA) to penetrate agar substrates. TDA simultaneously causes tegumental transformation similar to that seen when cercariae transform to schistosomula, reduces the Cercarienhüllen reaction in immune human serum, and reduces larval tolerance to water....
journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1016/j.exppara.2016.03.026
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
doi：10.1006/expr.1995.1100
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
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journal_title：Experimental parasitology
pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
pub_type： 杂志文章
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journal_title：Experimental parasitology
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