Trypanosoma cruzi: multiple actin isovariants are observed along different developmental stages.

Abstract:

:The expression and biological role of actin during the Trypanosoma cruzi life cycle remains largely unknown. Polyclonal antibodies against a recombinant T. cruzi actin protein were used to confirm its expression in epimastigotes, trypomastigotes, and amastigotes. Although the overall levels of expression were similar, clear differences in the subcellular distribution of actin among the developmental stages were identified. The existence of five actin variants in each developmental stage with distinct patterns of expression were uncovered by immunoblotting of protein extracts separated 2D-SDS gels. The isoelectric points of the actin variants in epimastigotes ranged from 4.45 to 4.9, whereas they ranged from 4.9 to 5.24 in trypomastigotes and amastigotes. To determine if the actin variants found could represent previously unidentified actins, we performed a genomic survey of the T.cruzi GeneDB database and found 12 independent loci encoding for a diverse group of actins and actin-like proteins that are conserved among trypanosomatids.

journal_name

Exp Parasitol

authors

Cevallos AM,Segura-Kato YX,Merchant-Larios H,Manning-Cela R,Alberto Hernández-Osorio L,Márquez-Dueñas C,Ambrosio JR,Reynoso-Ducoing O,Hernández R

doi

10.1016/j.exppara.2010.08.003

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

249-59

issue

1

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(10)00267-5

journal_volume

127

pub_type

杂志文章
  • Pancreatic hepatocytes in hamsters (Mesocricetus auratus) infected with Trypanosoma cruzi.

    abstract::Hepatocytic metaplasia may be induced in hamsters by carcinogens, and associated with aging, diabetes or chronic pancreatitis. By means of histopathologic and immunohistochemic studies, we observed pancreatic hepatocytes in hamsters infected and reinfected with Trypanosoma cruzi. The change was seen in 18 (19%) out of...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/S0014-4894(02)00005-X

    authors: dos Santos VM,de Lima MA,Cabrine-Santos M,Marquez Dde S,Reis Md,Pereira Gde A,Lages-Silva E,Ramírez LE

    更新日期:2002-02-01 00:00:00

  • Plasmodium vivax: polymorphism in the merozoite surface protein 1 gene from wild Colombian isolates.

    abstract::The Plasmodium vivax merozoite surface protein-1 (PvMSP-1) has been considered a candidate for a malaria vaccine against erythrocytic stages. PvMSP-1 is immunogenic during natural infections and exhibits antigenic polymorphism. The extent of genetic polymorphism in a region between the so-called interspecies conserved...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.2000.4534

    authors: Gutierrez A,Vicini J,Patarroyo ME,Murillo LA,Patarroyo MA

    更新日期:2000-07-01 00:00:00

  • Fasciolosis in India: An overview.

    abstract::Fasciolosis in ruminants is a relentless constraint in the livestock industry across the world. Immuno-prophylactic vaccines against fasciolosis may not come up in near future, rendering the control of this scourge with chemotherapy and snail population control. With the alarming threats of anti-fasciolid drug resista...

    journal_title:Experimental parasitology

    pub_type: 杂志文章,评审

    doi:10.1016/j.exppara.2021.108066

    authors: Lalrinkima H,Lalchhandama C,Jacob SS,Raina OK,Lallianchhunga MC

    更新日期:2021-01-12 00:00:00

  • Brugia malayi: the diagnostic potential of recombinant excretory/secretory antigens.

    abstract::The diagnostic potential of recombinant E/S antigens of the lymphatic filaria Brugia malayi was investigated by Western blot. A cDNA expression library was constructed using B. malayi male adult worm mRNA, and E/S recombinants were identified with a rabbit antiserum raised against E/S products collected in vitro from ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1110

    authors: Kumari S,Lillibridge CD,Bakeer M,Lowrie RC Jr,Jayaraman K,Philipp MT

    更新日期:1994-12-01 00:00:00

  • Biochemical, immunological, and biological characterization of Trypanosoma cruzi populations of the Andean north of Chile.

    abstract::Twenty-one Trypanosoma cruzi stocks isolated from Triatoma infestans and humans of the Chilean Andean highlands were studied at the genotypic level by schizodeme and molecular karyotype analyses, which allowed a clear distinction of the parasites from those hosts. A phenotypical characterization was performed by prote...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1995.1100

    authors: Gonzalez J,Muñoz S,Ortiz S,Anacona D,Salgado S,Galleguillos M,Neira I,Sagua H,Solari A

    更新日期:1995-08-01 00:00:00

  • Haemonchus contortus: molecular cloning, sequencing, and expression analysis of the gene coding for the small subunit of ribonucleotide reductase.

    abstract::Gastro-intestinal (GI) parasites are of great agricultural importance, annually costing the livestock industry vast amounts in resources to control parasitism. One such GI parasite, Haemonchus contortus, is principally pathogenic to sheep; with the parasite's blood-feeding behaviour causing effects ranging from mild a...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.08.001

    authors: Chen Y,Donald D,Savin K,Presidente PJ,Hartman D

    更新日期:2005-12-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from Plasmodium berghei: kinetic and electrophoretic characterization.

    abstract::Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(90)90108-o

    authors: Buckwitz D,Jacobasch G,Kuckelkorn U,Plonka A,Gerth C

    更新日期:1990-04-01 00:00:00

  • Schistosoma mansoni: protein phosphorylation during transformation of cercariae to schistosomula.

    abstract::Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90024-q

    authors: Wiest PM,Olds GR,Bowen WD

    更新日期:1991-08-01 00:00:00

  • Complete mitochondrial genomes of Dermacentor silvarum and comparative analyses with another hard tick Dermacentor nitens.

    abstract::Ticks are obligate blood-sucking ectoparasites of a wide range of vertebrates. They can transmit a range of pathogens that cause economic losses to livestock production as well as human disease. In the present study, the complete mitochondrial (mt) genome of Dermacentor silvarum was determined. The mt genome is 14,945...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.07.004

    authors: Guo DH,Zhang Y,Fu X,Gao Y,Liu YT,Qiu JH,Chang QC,Wang CR

    更新日期:2016-10-01 00:00:00

  • Leishmania panamensis: comparative inhibition of nuclear DNA topoisomerase II enzymes from promastigotes and human macrophages reveals anti-parasite selectivity of fluoroquinolones, flavonoids and pentamidine.

    abstract::Certain model inhibitors exerted selective action against the catalytic activity of nuclear DNA topoisomerase II (TOPII) of Leishmania panamensis promastigotes. The second-generation fluoroquinolones enoxacin and ciprofloxacin exhibited extraordinarily high anti-parasite selectivity displaying 582- and 40-fold greater...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2007.02.018

    authors: Cortázar TM,Coombs GH,Walker J

    更新日期:2007-08-01 00:00:00

  • Trypanosoma brucei: two-dimensional gel analysis of the major glycosomal proteins during the life cycle.

    abstract::Kinetoplastid organisms possess a unique organelle, the glycosome, which compartmentalizes the Embden-Meyerhof segment of glycolysis and several other metabolic pathways. In Trypanosoma brucei many of the enzyme activities localized to the glycosome are stage regulated. Two-dimensional gel analysis was used to examine...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(90)90109-p

    authors: Parsons M,Nielsen B

    更新日期:1990-04-01 00:00:00

  • Peptidases of pinworms Syphacia muris and Passalurus ambiguus.

    abstract::In this first report about pinworms peptidases we primarily characterize peptidases released during in vitro maintenance of two common pinworms of laboratory animals -Syphacia muris and Passalurus ambiguus. The peptidase activity obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showe...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2010.04.018

    authors: Vadlejch J,Lytvynets A,Jankovská I,Langrová I

    更新日期:2010-10-01 00:00:00

  • Schistosoma mansoni: possible involvement of protein kinase C in linoleic acid-induced proteolytic enzyme release from cercariae.

    abstract::The possible involvement of protein kinase C and Ca2+ metabolism in the proteolytic enzyme release from schistosome cercariae was studied. Cercariae were placed in dechlorinated tap water containing 0.37 mM calcium in the small glass petri dish and exposed to the stimuli (linoleic acid, phorbol esters, and Ca2+ ionoph...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90151-l

    authors: Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Y

    更新日期:1991-04-01 00:00:00

  • Plasmodium falciparum: development and validation of a measure of intraerythrocytic growth using SYBR Green I in a flow cytometer.

    abstract::Reliable analytical techniques to test growth-promoting and antimalarial efficacy on plasmodia are very important. Flow cytometry (FCM) offers the possibility to study developmental stages of intraerythrocytic growth of malaria parasites using nucleic acid staining. To analyze the growth of Plasmodium falciparum SYBR ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.10.008

    authors: Izumiyama S,Omura M,Takasaki T,Ohmae H,Asahi H

    更新日期:2009-02-01 00:00:00

  • Schistosoma mansoni: functional proteasomes are required for development in the vertebrate host.

    abstract::Proteasomes are multi-subunit proteases involved in several mechanisms and thought to contribute to the regulation of cellular homeostasis. Here, we report for the first time biochemical evidence for the existence of a ubiquitin-proteasome proteolytic pathway in this parasite. Proteasomes from both cercariae and adult...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.01.002

    authors: Guerra-Sá R,Castro-Borges W,Evangelista EA,Kettelhut IC,Rodrigues V

    更新日期:2005-04-01 00:00:00

  • Synthesis, antimalarial activity and molecular docking of hybrid 4-aminoquinoline-1,3,5-triazine derivatives.

    abstract::A series of novel hybrid 4-aminoquinoline 1,3,5-triazine derivatives was synthesized in a five-steps reaction and evaluated for their in vitro antimalarial activity against chloroquine-sensitive (3D7) and chloroquine-resistant (RKL-2) strains of Plasmodium falciparum. Entire synthetic derivatives showed higher antimal...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2015.06.016

    authors: Bhat HR,Singh UP,Thakur A,Kumar Ghosh S,Gogoi K,Prakash A,Singh RK

    更新日期:2015-10-01 00:00:00

  • Effects of IFN-γ, TNF-α, IL-10 and TGF-β on Neospora caninum infection in rat glial cells.

    abstract::Neospora caninum causes abortion in cattle and neurological disorders in dogs. The immunological response to this parasite has been described as predominantly of the Th1 type. However, infected primary glial cell cultures release IL-10 and IL-6 but not IFN-γ. This suggests a rather protective response of the glia to a...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.11.016

    authors: Jesus EE,Pinheiro AM,Santos AB,Freire SM,Tardy MB,El-Bachá RS,Costa SL,Costa MF

    更新日期:2013-03-01 00:00:00

  • Plasmodium falciparum: the Pf332 antigen is secreted from the parasite by a brefeldin A-dependent pathway and is translocated to the erythrocyte membrane via the Maurer's clefts.

    abstract::The transport of the megadalton protein Pf332 was studied during the asexual bloodstage development of Plasmodium falciparum. Four mouse monoclonal antibodies, produced against a recombinant polypeptide derived from the Pf332 protein, were used to analyze the kinetics of synthesis, the subcellular location, and transp...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1091

    authors: Hinterberg K,Scherf A,Gysin J,Toyoshima T,Aikawa M,Mazie JC,da Silva LP,Mattei D

    更新日期:1994-11-01 00:00:00

  • Plasmodium gallinaceum: sporozoite invasion of Aedes aegypti salivary glands is inhibited by anti-gland antibodies and by lectins.

    abstract::There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1995.1124

    authors: Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KD

    更新日期:1995-11-01 00:00:00

  • The therapeutic potential of phytol towards Trypanosoma congolense infection and the inhibitory effects against trypanosomal sialidase.

    abstract::The search for novel therapeutic candidates against animal trypanosomiasis is an ongoing scientific endevour because of the negative impacts of the disease to the African livestock industry. In this study, the in vivo therapeutic potentials of phytol toward Trypanosoma congolense infection and the inhibitory effects o...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107943

    authors: Saad SB,Ibrahim MA,Jatau ID,Shuaibu MN

    更新日期:2020-09-01 00:00:00

  • A defined medium for Leishmania culture allows definition of essential amino acids.

    abstract::Axenic culture of Leishmania is generally performed in rich, serum-supplemented media which sustain robust growth over multiple passages. The use of such undefined media, however, obscures proteomic analyses and confounds the study of metabolism. We have established a simple, defined culture medium that supports the s...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2018.01.009

    authors: Nayak A,Akpunarlieva S,Barrett M,Burchmore R

    更新日期:2018-02-01 00:00:00

  • Flow cytometric screening for anti-leishmanials in a human macrophage cell line.

    abstract::High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2010.06.007

    authors: Mehta SR,Zhang XQ,Badaro R,Spina C,Day J,Chang KP,Schooley RT

    更新日期:2010-12-01 00:00:00

  • Sensitivity testing of trypanosome detection by PCR from whole blood samples using manual and automated DNA extraction methods.

    abstract::Automated extraction of DNA for testing of laboratory samples is an attractive alternative to labour-intensive manual methods when higher throughput is required. However, it is important to maintain the maximum detection sensitivity possible to reduce the occurrence of type II errors (false negatives; failure to detec...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2014.08.006

    authors: Dunlop J,Thompson CK,Godfrey SS,Thompson RC

    更新日期:2014-11-01 00:00:00

  • Angiostrongylus cantonensis: identification and characterization of microRNAs in male and female adults.

    abstract::Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and patho...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.02.019

    authors: Chen MX,Ai L,Xu MJ,Zhang RL,Chen SH,Zhang YN,Guo J,Cai YC,Tian LG,Zhang LL,Zhu XQ,Chen JX

    更新日期:2011-06-01 00:00:00

  • Trypanosoma brucei: a survey of pyrimidine transport activities.

    abstract::Purine uptake has been studied in many protozoan parasites in the last few years, and several of the purine transporters have been cloned. In contrast, very little is known about the salvage of preformed pyrimidines by protozoa, and no pyrimidine transporters have been cloned, yet chemotherapy based on pyrimidine nucl...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2006.02.018

    authors: Gudin S,Quashie NB,Candlish D,Al-Salabi MI,Jarvis SM,Ranford-Cartwright LC,de Koning HP

    更新日期:2006-10-01 00:00:00

  • Hymenolepis diminuta: lack of sulphate activation and sulphotransferase activity.

    abstract::There is no evidence that Hymenolepis diminuta can carry out sulphoconjugation reactions. Neither whole worms nor worm extracts were able to sulphate 4-methylumbelliferone. No sulphotransferase activity could be demonstrated in H. diminuta using a variety of substrates, nor was H. diminuta capable of synthesising the ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(88)90124-5

    authors: Raines PS,Barrett J

    更新日期:1988-04-01 00:00:00

  • Plasmodium chabaudi chabaudi: B-1 cell expansion correlates with semiresistance in BALB/cJ mice.

    abstract::The largest obstacle impeding the development of an effective malaria vaccine is the incomplete understanding of how the immune response is regulated during infection. B-1a cells, a poorly understood subcategory of B lymphocytes, produce nonpathologic autoantibodies of low affinity which have been shown to have distin...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.2001.4622

    authors: Yoder BJ,Goodrum KJ

    更新日期:2001-06-01 00:00:00

  • A family of serine protease inhibitors (serpins) in the cattle tick Rhipicephalus (Boophilus) microplus.

    abstract::Proteins belonging to the serine protease inhibitor (serpin) superfamily play essential roles in many organisms. In arthropods these proteins are involved in innate immune system, morphogenesis and development. In mammals serpins regulate pathways that are essential to life such as blood coagulation, fibrinolysis, inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2013.12.001

    authors: Tirloni L,Seixas A,Mulenga A,Vaz Ida S Jr,Termignoni C

    更新日期:2014-02-01 00:00:00

  • Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene.

    abstract::The thymidine kinase gene of Herpes simplex type-1 virus was transfected into several Leishmania species to create drug-sensitive mutants. Expression of the thymidine kinase gene is not by itself harmful to Leishmania cells but it is capable of phosphorylating ganciclovir, a nucleoside analog, into a highly toxic prod...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.4115

    authors: Muyombwe A,Olivier M,Ouellette M,Papadopoulou B

    更新日期:1997-01-01 00:00:00

  • Proteolytic and nematicidal potential of the compost colonized by Hypsizygus marmoreus.

    abstract::Spent mushroom compost (SMC) is a residue generated in edible mushrooms production, such as Hypsizygus marmoreus. Its genome was recently sequenced, demonstrating cuticle-degrading protease genes. The present work aims to investigate the proteases from H. marmoreus spent mushroom compost (SMC) by verifying its action ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2018.12.006

    authors: Soares FEF,Nakajima VM,Sufiate BL,Satiro LAS,Gomes EH,Fróes FV,Sena FP,Braga FR,de Queiroz JH

    更新日期:2019-02-01 00:00:00